Increasing Thermal Stability of Gelatin by UV-Induced Cross-Linking with Glucose

The effects of ultraviolet (254 nm) radiation on a hydrated gelatin-glucose matrix were investigated for the development of a physiologically thermostable substrate for potential use in cell scaffold production. Experiments conducted with a differential scanning calorimeter indicate that ultraviolet irradiation of gelatin-glucose hydrogels dramatically increases thermal stability such that no melting is observed at temperatures of at least 90°C. The addition of glucose significantly increases the yield of cross-linked product, suggesting that glucose has a role in cross-link formation. Comparisons of lyophilized samples using scanning electron microscopy show that irradiated materials have visibly different densities.

[1]  S. W. Lin,et al.  Protein damage and degradation by oxygen radicals. II. Modification of amino acids. , 1987, The Journal of biological chemistry.

[2]  F. Barry,et al.  Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. , 2000, Journal of biomedical materials research.

[3]  D. Prockop,et al.  The collagen fibril: the almost crystalline structure. , 1998, Journal of structural biology.

[4]  J. A. Chapman,et al.  Collagen fibril formation. , 1996, The Biochemical journal.

[5]  Mina J Bissell,et al.  Modeling tissue-specific signaling and organ function in three dimensions , 2003, Journal of Cell Science.

[6]  S. Damrongsakkul,et al.  Comparison of gelatin and collagen scaffolds for fibroblast cell culture , 2006 .

[7]  J. Eastoe,et al.  The amino acid composition of mammalian collagen and gelatin. , 1955, The Biochemical journal.

[8]  G. Ya. Wiederschain,et al.  Handbook of Biochemistry and Molecular Biology , 2010, Biochemistry (Moscow).

[9]  S. Hsu,et al.  Viscoelastic studies of extracellular matrix interactions in a model native collagen gel system. , 1994, Biorheology.

[10]  M. Raghunath,et al.  Electro-spinning of pure collagen nano-fibres - just an expensive way to make gelatin? , 2008, Biomaterials.

[11]  S. Van Vlierberghe,et al.  Use of a gelatin cryogel as biomaterial scaffold in the differentiation process of human bone marrow stromal cells , 2010, 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology.

[12]  I. Yannas,et al.  Antigenicity and immunogenicity of collagen. , 2004, Journal of biomedical materials research. Part B, Applied biomaterials.

[13]  T. Yamaoka,et al.  Beating behavior of primary neonatal cardiomyocytes and cardiac-differentiated P19.CL6 cells on different extracellular matrix components , 2009, Journal of Artificial Organs.

[14]  L. Mosca,et al.  The oxidation of oxytocin and vasopressin by peroxidase/H2O2 system , 1995, Amino Acids.

[15]  T. Oppenländer.  Photochemical Processes of Water Treatment , 2007 .

[16]  D. Cooper,et al.  The effect of ultraviolet irradiation on soluble collagen. , 1965, The Biochemical journal.

[17]  H. L. Mason,et al.  The antigenicity of collagen. , 1949, Journal of immunology.

[18]  Koji Takahashi,et al.  Soft Textural and Emulsifiable Gelatin Formed by Conjugating with Fatty-Acylated Saccharide , 2008, Bioscience, biotechnology, and biochemistry.

[19]  A. Sionkowska,et al.  Spectroscopic studies into the influence of UV radiation on elastin hydrolysates in water solution. , 2006, Journal of photochemistry and photobiology. B, Biology.

[20]  Yan Huang,et al.  In vitro characterization of chitosan-gelatin scaffolds for tissue engineering. , 2005, Biomaterials.

[21]  J T Czernuszka,et al.  Novel 3D collagen scaffolds fabricated by indirect printing technique for tissue engineering. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[22]  D. Ross,et al.  A Potential Role for Cyclized Quinones Derived from Dopamine, DOPA, and 3,4-Dihydroxyphenylacetic Acid in Proteasomal Inhibition , 2006, Molecular Pharmacology.

[23]  M. Dunn,et al.  Synergistic effects of glucose and ultraviolet irradiation on the physical properties of collagen. , 2002, Journal of biomedical materials research.

[24]  M. Shoichet,et al.  Design of three-dimensional biomimetic scaffolds. , 2010, Journal of biomedical materials research. Part A.

[25]  E. Fujimori,et al.  Ultraviolet light‐induced change in collagen macromolecules , 1965, Biopolymers.

[26]  Christian Schuetz,et al.  Regeneration and orthotopic transplantation of a bioartificial lung , 2010, Nature Medicine.

[27]  G. Phillips,et al.  Photodegradation of carbohydrates. Part IV. Direct photolysis of D-glucose in aqueous solution , 1969 .

[28]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[29]  Jiaoyan Ren,et al.  Effect of xylose on the molecular and particle size distribution of peanut hydrolysate in Maillard reaction system. , 2011, Journal of the science of food and agriculture.

[30]  M. Kameyama,et al.  Age-related differences in human skin collagen: solubility in solvent, susceptibility to pepsin digestion, and the spectrum of the solubilized polymeric collagen molecules. , 1982, Journal of gerontology.

[31]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[32]  H. Kubota,et al.  Photo-Induced Formation of Peroxide in Saccharides and Related Compounds , 1976 .

[33]  Sanskrita Das,et al.  Enhanced redifferentiation of chondrocytes on microperiodic silk/gelatin scaffolds: toward tailor-made tissue engineering. , 2013, Biomacromolecules.

[34]  E. J. Miller,et al.  Physical crosslinking of collagen fibers: comparison of ultraviolet irradiation and dehydrothermal treatment. , 1995, Journal of biomedical materials research.

[35]  C. Sonntag,et al.  The photolysis (λ = 254 nm) of tyrosine in aqueous solutions in the absence and presence of oxygen. The reaction of tyrosine with singlet oxygen☆ , 1995 .

[36]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .

[37]  Brenda J. Schuler.  Evaluation of novel cross -linking agents for gelatin/collagen matrices , 2004 .

[38]  P. V. von Hippel,et al.  Calculation of protein extinction coefficients from amino acid sequence data. , 1989, Analytical biochemistry.

[39]  Julie Glowacki,et al.  Collagen scaffolds for tissue engineering. , 2008, Biopolymers.

[40]  S. Hill,et al.  Maillard induced complexes of bovine serum albumin--a dilute solution study. , 1996, International journal of biological macromolecules.

[41]  Kenneth M. Yamada,et al.  Taking Cell-Matrix Adhesions to the Third Dimension , 2001, Science.

[42]  Sangeeta N Bhatia,et al.  Fabrication of three-dimensional tissues. , 2007, Advances in biochemical engineering/biotechnology.

[43]  D. Hulmes,et al.  Quasi-hexagonal molecular packing in collagen fibrils , 1979, Nature.

[44]  P. Bechtel,et al.  Ultraviolet-B radiation induced cross-linking improves physical properties of cold- and warm-water fish gelatin gels and films. , 2012, Journal of food science.

[45]  Y. Ikada,et al.  Cross-Linking and Biodegradation of Native and Denatured Collagen , 1993 .

[46]  D A Parry,et al.  Analysis of the primary structure of collagen for the origins of molecular packing. , 1973, Journal of molecular biology.

[47]  S. Davis,et al.  Sugar cross-linked gelatin for controlled release: microspheres and disks. , 1998, Biomaterials.

[48]  A. Veis,et al.  The long range reorganization of gelatin to the collagen structure. , 1961, Archives of biochemistry and biophysics.

[49]  Ann Marie Schmidt,et al.  Advanced glycation end products: sparking the development of diabetic vascular injury. , 2006, Circulation.

[50]  Paulo Jorge Da Silva bartolo,et al.  Biofabrication Strategies for Tissue Engineering , 2011 .

[51]  M. Lederer,et al.  Cross-linking of proteins by Maillard processes--model reactions of D-glucose or methylglyoxal with butylamine and guanidine derivatives. , 1998, Bioorganic & medicinal chemistry.

[52]  U. Wegst,et al.  Structure-property-processing correlations in freeze-cast composite scaffolds. , 2013, Acta biomaterialia.

[53]  A. Ohara,et al.  Tyrosine formation from phenylalanine by ultraviolet irradiation. , 1990, Chemical & pharmaceutical bulletin.