Agar diffusion method for toxicity screening of plastics on cultured cell monolayers.

In a previous paper, a tissue culture method (fluid-medium) was used to screen the toxicity of plastic materials to be used in medical practice. Even though this method was found to be very acceptable for most plastic materials, some difficulties were encountered when plastics having low densities or plastics having odd shapes or forms were tested. For this reason an agar-diffusion method was adapted which could be used for the testing of any type of plastic as well as for the testing of solid and liquid extracts.