Structure elucidation and biological property of minor antibiotic in Streptomyces hygroscopicus NND-52

PurposeThe aim is to elucidate the structure of minor antibiotic in Streptomyces hygroscopicus NND 52 and to test its in vitro biological property. MethodsStructure elucidation was based on the analysis of UV, EI MS, IR, NMR. Checkerboard Microdilution and dilution were used to determine MIC and PAE. ResultsM2 was identical with Nigericin. This antibiotic was effective against Gram positive bacteria,including resistant clinical bacteria. It could kill almost all bacteria in 24 h and PAE was rather satisfying.ConclusionM2 can be a new strategy in the therapy to resistant clinical bacteria.