Field observations on the variation of Streptococcus uberis populations in a pasture-based dairy farm.

Microbiological and molecular tools were used to monitor Streptococcus uberis populations on farm tracks and paddocks on a dairy farm during different seasons of a year to identify and profile potential environmental niches of Strep. uberis in a pasture-based dairying system. Farm tracks of high or low cow traffic were sampled every 2 wk for an entire year and Strep. uberis numbers were enumerated from a selective medium. During each season of the year, paddocks were sampled for the presence of Strep. uberis before and after grazing by dairy cows. Farm tracks of high cow traffic generally had greater concentrations of Strep. uberis isolated compared with tracks with less cow traffic, but there was also significant variation in the concentrations of Strep. uberis contamination among seasons, being highest in winter and lowest in summer. The bacterium was detected in paddocks only after cow grazing had occurred, but the bacteria could still be detected in soil for up to 2 wk following grazing in winter. Multilocus sequence typing showed great heterogeneity, with some commonality between farm track and milk isolates, which may help explain cow-to-environment or environment-to-cow transmission of the bacterium in the dairy setting.

[1]  M. Maiden,et al.  Multilocus-sequence typing analysis reveals similar populations of Streptococcus uberis are responsible for bovine intramammary infections of short and long duration. , 2007, Veterinary microbiology.

[2]  R. Almeida,et al.  Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM). , 2006, Veterinary microbiology.

[3]  M. Maiden,et al.  Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom , 2006, Applied and Environmental Microbiology.

[4]  Keith A. Jolley,et al.  First Insights into the Evolution of Streptococcus uberis: a Multilocus Sequence Typing Scheme That Enables Investigation of Its Population Biology , 2006, Applied and Environmental Microbiology.

[5]  R. Zadoks,et al.  Ribotyping of Streptococcus uberis from a dairy's environment, bovine feces and milk. , 2005, Veterinary microbiology.

[6]  M. Hutchison,et al.  Fate of Pathogens Present in Livestock Wastes Spread onto Fescue Plots , 2005, Applied and Environmental Microbiology.

[7]  S. Saggar,et al.  A review of emissions of methane, ammonia, and nitrous oxide from animal excreta deposition and farm effluent application in grazed pastures , 2004 .

[8]  S. McDougall.  Intramammary treatment of clinical mastitis of dairy cows with a combination of lincomycin and neomycin, or penicillin and dihydrostreptomycin , 2003, New Zealand veterinary journal.

[9]  R. Zadoks,et al.  Clinical, epidemiological and molecular characteristics of Streptococcus uberis infections in dairy herds , 2003, Epidemiology and Infection.

[10]  G. Verkerk.  Pasture-based dairying: challenges and rewards for New Zealand producers. , 2003, Theriogenology.

[11]  J. Leigh,et al.  The Hyaluronic Acid Capsule of Streptococcus uberis Is Not Required for the Development of Infection and Clinical Mastitis , 2003, Infection and Immunity.

[12]  S. McDougall.  Bovine mastitis: epidemiology, treatment and control , 2002, New Zealand veterinary journal.

[13]  H. Allore,et al.  Cow- and quarter-level risk factors for Streptococcus uberis and Staphylococcus aureus mastitis. , 2001, Journal of dairy science.

[14]  S. Waage,et al.  Case-control study of risk factors for clinical mastitis in postpartum dairy heifers. , 2001, Journal of dairy science.

[15]  A. Hope.  Laboratory Handbook on Bovine Mastitis. , 2000 .

[16]  S. McDougall.  Efficacy of two antibiotic treatments in curing clinical and subclinical mastitis in lactating dairy cows. , 1998, New Zealand veterinary journal.

[17]  R. Almeida,et al.  Virulence factors of Streptococcus uberis isolated from cows with mastitis. , 1998, Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B.

[18]  Woolford Mw,et al.  The prevalence of mastitis in primiparous heifers in eleven Waikato dairy herds. , 1996 .

[19]  J. Hogan,et al.  Environmental streptococcal intramammary infections of the bovine mammary gland. , 1995, Journal of dairy science.

[20]  J. Williamson,et al.  The prophylactic effect of a dry-cow antibiotic against Streptococcus uberis. , 1995, New Zealand veterinary journal.

[21]  W. Powers,et al.  Components of dairy manure management systems. , 1994, Journal of dairy science.

[22]  R. Harmon,et al.  Mastitis in heifers. , 1993, The Veterinary clinics of North America. Food animal practice.

[23]  R. Miller,et al.  Increased susceptibility to intramammary infection following removal of teat canal keratin. , 1992, Journal of dairy science.

[24]  J. Seinhorst,et al.  Effect of damage to the teat end on the experimental induction of mastitis in dry cows with Corynebacterium pyogenes , 1991, Veterinary Record.

[25]  M. A. Campbell,et al.  Incidence and types of clinical mastitis in dairy herds with high and low somatic cell counts. , 1988, Journal of the American Veterinary Medical Association.

[26]  K. Smith.  Mastitis control: a discussion. , 1983, Journal of Dairy Science.

[27]  R. J. Grindal,et al.  Evidence of penetration of the bovine teat duct by Escherichia coli in the interval between milkings , 1981, Journal of Dairy Research.

[28]  H. D. Patterson,et al.  Recovery of inter-block information when block sizes are unequal , 1971 .

[29]  J. Williamson,et al.  Teat spraying prior to calving may reduce the risk of heifer mastitis caused by Streptococcus uberis , 2006 .

[30]  Henk Hogeveen,et al.  Mastitis in dairy production. Current knowledge and future solutions , 2005 .

[31]  J. Williamson,et al.  Associations between Streptococcus uberis populations on farm races and climatic changes during a twelve-month period , 2005 .

[32]  J. Williamson,et al.  Isolation of Streptococcus uberis from different sites of the dairy cow. , 2005 .

[33]  D. Döpfer,et al.  Optimised sample sizes for analysing the genetic heterogeneity of mammary pathogen isolates from environmental samples. , 2005 .

[34]  MacDonald Ka,et al.  Management decision rules to optimise milksolids production on dairy farms , 1998 .

[35]  J. Williamson,et al.  The prevalence of mastitis in primiparous heifers in eleven Waikato dairy herds. , 1996, New Zealand veterinary journal.