Variations in the abundance of 24 protein biomarkers of beef tenderness according to muscle and animal type.

Some proteins have been revealed as biomarkers for beef tenderness by previous studies. These markers could be used in immunological tests to predict beef tenderness, in living animals as well as in carcasses. It is well known that rearing practices modify the amounts of mRNA and proteins. Therefore, the reliability of protein tests could be affected by livestock and biological effects such as production systems, breed, muscle and animal type. This study analysed the effects of animal and muscle type on 24 proteins. The animals studied were 67 young bulls and 44 steers of the Charolais breed, and muscles were Longissimus thoracis and Semitendinosus. Protein amounts were determined by Dot blot, an immunological technique. Results showed that expressions of 20 proteins were influenced by animal and/or muscle type. These results could lead to modifications and adaptations of prediction tests according to rearing practice, bovine breed and beef cut.

[1]  B. Picard,et al.  Different phenotypic and proteomic markers explain variability of beef tenderness across muscles , 2012 .

[2]  B. Picard,et al.  Expression of DNAJA1 in bovine muscles according to developmental age and management factors. , 2011, Animal : an international journal of animal bioscience.

[3]  N. Guillemin.  Marqueurs protéiques de la tendreté de la viande bovine: étude prédictive et fonctionnelle , 2010 .

[4]  C. Berri,et al.  Skeletal muscle proteomics in livestock production. , 2010, Briefings in functional genomics.

[5]  L. Lefaucheur.  A second look into fibre typing--relation to meat quality. , 2010, Meat science.

[6]  Yukie Tanaka,et al.  Protein expression profiling of lens epithelial cells from Prdx6-depleted mice and their vulnerability to UV radiation exposure. , 2010, American journal of physiology. Cell physiology.

[7]  J. Hocquette,et al.  Pasture-feeding of Charolais steers influences skeletal muscle metabolism and gene expression. , 2009, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[8]  B. Picard,et al.  Validation of a Dot-Blot quantitative technique for large scale analysis of beef tenderness biomarkers. , 2009, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[9]  K. Hollung,et al.  Peroxiredoxin-6--a potential protein marker for meat tenderness in bovine longissimus thoracis muscle. , 2009, Journal of animal science.

[10]  N. Guillemin,et al.  La maîtrise de la tendreté de la viande bovine: identification de marqueurs biologiques , 2009 .

[11]  Byoung-Chul Kim,et al.  Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality , 2009 .

[12]  B. Picard,et al.  Meta-analysis of the effect of animal maturity on muscle characteristics in different muscles, breeds, and sexes of cattle. , 2008, Journal of animal science.

[13]  B. Picard,et al.  Application of gene expression studies in livestock production systems: a European perspective , 2008 .

[14]  E. Dransfield,et al.  Muscle and meat quality characteristics of Holstein and Salers cull cows. , 2007, Meat science.

[15]  R. Griffiths,et al.  The role of hormones, cytokines and heat shock proteins during age-related muscle loss. , 2007, Clinical nutrition.

[16]  Ueli Aebi,et al.  Intermediate filaments: from cell architecture to nanomechanics , 2007, Nature Reviews Molecular Cell Biology.

[17]  M. Le Cunff,et al.  New indicators of beef sensory quality revealed by expression of specific genes. , 2007, Journal of agricultural and food chemistry.

[18]  E. Dransfield,et al.  Muscle and meat characteristics from the main beef breeds of the Massif Central , 2007 .

[19]  S. Kaul,et al.  Three faces of mortalin: A housekeeper, guardian and killer , 2007, Experimental Gerontology.

[20]  B. Picard,et al.  Recent advances in cattle functional genomics and their application to beef quality. , 2007, Animal : an international journal of animal bioscience.

[21]  C. Morris,et al.  Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey x Limousin, Angus and Hereford-cross cattle. , 2006, Animal genetics.

[22]  K. Hollung,et al.  Proteome analysis of early post‐mortem changes in two bovine muscle types: M. longissimus dorsi and M. semitendinosis , 2006, Proteomics.

[23]  Y. Sun,et al.  Small heat shock proteins: molecular structure and chaperone function , 2005, Cellular and Molecular Life Sciences CMLS.

[24]  Xin Zhao,et al.  Interaction of DJ-1 with Daxx inhibits apoptosis signal-regulating kinase 1 activity and cell death. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[25]  Patrick Veysset,et al.  Adaptation to Agenda 2000 (CAP reform) and optimisation of the farming system of French suckler cattle farms in the Charolais area: a model-based study , 2005 .

[26]  D. Paulin,et al.  Desmin: a major intermediate filament protein essential for the structural integrity and function of muscle. , 2004, Experimental cell research.

[27]  B. Picard,et al.  Mapping of bovine skeletal muscle proteins using two‐dimensional gel electrophoresis and mass spectrometry , 2004, Proteomics.

[28]  H. J. Andersen,et al.  Postmortem proteome changes of porcine muscle related to tenderness. , 2003, Journal of agricultural and food chemistry.

[29]  J. N. Stallone,et al.  Gender differences in the expression of heat shock proteins: the effect of estrogen. , 2003, American journal of physiology. Heart and circulatory physiology.

[30]  K. Forsman-Semb,et al.  Mice with Targeted Mutation of Peroxiredoxin 6 Develop Normally but Are Susceptible to Oxidative Stress* , 2003, Journal of Biological Chemistry.

[31]  E. Dransfield,et al.  Meat quality and composition of three muscles from French cull cows and young bulls , 2003 .

[32]  J. Hocquette,et al.  Effects of muscle type, castration, age and growth rate on H-FABP expression in bovine skeletal muscle , 2002 .

[33]  M. Perng,et al.  Comparison of the small heat shock proteins αB-crystallin, MKBP, HSP25, HSP20, and cvHSP in heart and skeletal muscle , 2000, Histochemistry and Cell Biology.

[34]  B. Picard,et al.  Electrophoretic separation of bovine muscle myosin heavy chain isoforms. , 1999, Meat science.

[35]  J. Reinstein,et al.  Mechanism of regulation of hsp70 chaperones by DnaJ cochaperones. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[36]  A. Fink.  Chaperone-mediated protein folding. , 1999, Physiological reviews.

[37]  J. Kiang,et al.  Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. , 1998, Pharmacology & therapeutics.

[38]  M. Prevost,et al.  A missense mutation in the αB-crystallin chaperone gene causes a desmin-related myopathy , 1998, Nature Genetics.

[39]  H. Steinhart,et al.  Differences in natural steroid hormone patterns of beef from bulls and steers. , 1998, Journal of animal science.

[40]  P. Piper,et al.  The molecular defences against reactive oxygen species in yeast , 1996, Molecular microbiology.

[41]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[42]  B. Picard,et al.  Control of beef tenderness: identification of biological markers. , 2009 .

[43]  A. Ouali,et al.  Caracteristiques des muscles et de la viande des principales races bovines allaitantes du massif central , 2006 .

[44]  H. Kryvi,et al.  Distribution patterns of muscle fibre types in major muscles of the bull (Bos taurus) , 2005, Anatomy and Embryology.

[45]  J. Bouley.  Analyse protéomique du muscle squelettique bovin , 2004 .

[46]  M. Beckerle,et al.  Striated muscle cytoarchitecture: an intricate web of form and function. , 2002, Annual review of cell and developmental biology.

[47]  J. Hocquette,et al.  Effect of nutritional factors on biochemical, structural and metabolic characteristics of muscles in ruminants, consequences on dietetic value and sensorial qualities of meat. , 2001, Reproduction, nutrition, development.

[48]  J. Cohen,et al.  Identification of the A-band localization domain of myosin binding proteins C and H (MyBP-C, MyBP-H) in skeletal muscle. , 1999, Journal of cell science.

[49]  B. Picard,et al.  Muscle fibre characteristics in four muscles of growing male cattle: II. Effect of castration and feeding level , 1998 .

[50]  B. Picard,et al.  Inter-animal variation in the biological characteristics of muscle tissue in male limousin cattle. , 1995, Meat science.