Degradation of poly aromatic fractions of crude oil and detection of catabolic genes in hydrocarbon-degrading bacteria isolated from Agbabu bitumen sediments in Ondo State

Pollution due to release of Poly aromatic hydrocarbons (PAHs) are a major environmental issue especially in oil producing communities. This study investigates the polyaromatic hydrocarbon degradation potentials of some bacteria: Campylobacter hominis, Bacillus cereus, Dyadobacter koreensis, Pseudomonas aeruginosa and Micrococcus luteus isolated from Agbabu bitumen sediments in Ondo State. The isolates were used singly and in consortium for the degradation of Bonny light crude oil. Concentrations of residual aromatic hydrocarbons in crude oil degraded by these isolates were determined by Gas chromatography/Mass Spectroscopy with flame ionization detector (FID). Detection of catabolic genes (nahH, CatA and AlkB) in the isolates was determined by PCR amplification of their specific primers. The GC-MS analyses showed degradation of poly aromatic hydrocarbons (PAHs) by these isolates. The consortium exhibited the highest PAH reduction (73%) while C. hominis had the least PAH reduction (56%). Dyadobacter koreensis, P. aeruginosa, Micrococcus luteus and B. cereus, displayed 66%, 60%, 59% and 58% PAH reduction respectively. The catabolic gene nahH gene was present in B. cereus, D. koreensis, P. aeruginosa and M. luteus, alkB gene was present in B. cereus, C. hominis, and D. koreensis while CatA was not detected in any of the isolates. The findings of this study affirmed the hydrocarbon-degrading abilities and presence of catabolic genes in these bacteria, these make them potential tools in oil prospecting and cleaning up of hydrocarbon contaminated sites.

[1]  R. Naidu,et al.  Toxicity assessment of fresh and weathered petroleum hydrocarbons in contaminated soil- a review. , 2018, Chemosphere.

[2]  Y. Gong,et al.  Degradation of petroleum hydrocarbons in seawater by simulated surface-level atmospheric ozone: Reaction kinetics and effect of oil dispersant. , 2018, Marine pollution bulletin.

[3]  J. Oluyege,et al.  Catechol-2,3-dioxygenase and Lipase Activities during Degradation of Crude Oil by Hydrocarbon-degrading Bacteria Isolated from Bitumen-polluted Surface Water in Agbabu, Ondo State , 2018 .

[4]  R. Taheri,et al.  Enhanced biodegradation of phenol by magnetically immobilized Trichosporon cutaneum , 2018, Annals of Microbiology.

[5]  T. Chitov,et al.  Assessment of bacterial communities and activities of thermotolerant enzymes produced by bacteria indigenous to oil-bearing sandstone cores for potential application in Enhanced Oil Recovery , 2018 .

[6]  S. Kumari,et al.  Improved polycyclic aromatic hydrocarbon degradation in a crude oil by individual and a consortium of bacteria. , 2018, Bioresource technology.

[7]  E. Muge,et al.  Screening and Characterization of Hydrocarbonoclastic Bacteria Isolated from Oil-contaminated Soils from Auto Garages , 2018 .

[8]  A. Gideon,et al.  Laboratory-Scale Bioremediation of Crude Oil Polluted Soil Using a Consortia of Rhizobacteria Obtained from Plants in Gokana-Ogoni, Rivers State , 2018 .

[9]  Kengo Inoue,et al.  Synergistic degradation of pyrene by five culturable bacteria in a mangrove sediment-derived bacterial consortium. , 2018, Journal of hazardous materials.

[10]  T. Olowomofe,et al.  Isolation, Screening and Characterization of Hydrocarbon-Utilizing Bacteria Isolated from Bitumen-Contaminated Surface Water in Agbabu, Ondo State , 2017 .

[11]  A. Odeyemi,et al.  Growth Patterns and Degradative Potentials of Pseudomonas sp. Isolated from Waste Dumpsite Soil in Crude Oil Supplemented Soil Extract and Mineral Salts Media , 2017 .

[12]  Vipin Kumar,et al.  Biodegradation of Polycyclic Aromatic Hydrocarbons by Microbial Consortium: A Distinctive Approach for Decontamination of Soil , 2016 .

[13]  Sanket Joshi,et al.  Microbial enhanced heavy crude oil recovery through biodegradation using bacterial isolates from an Omani oil field , 2015, Microbial Cell Factories.

[14]  G. Zafra,et al.  Morphological changes and growth of filamentous fungi in the presence of high concentrations of PAHs , 2015, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[15]  R. Aguilar-López,et al.  Evaluation of hydrocarbons and organochlorine pesticides and their tolerant microorganisms from an agricultural soil to define its bioremediation feasibility , 2015, Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes.

[16]  F. A. Adekola,et al.  Biodegradation of hydrocarbon compounds in Agbabu natural bitumen , 2014 .

[17]  Man-li Wu,et al.  Degradation of polycyclic aromatic hydrocarbons by microbial consortia enriched from three soils using two different culture media. , 2013, Environmental pollution.

[18]  J. Gong,et al.  A novel benzoate-degrading Rhodococcus strain contains three catA genes with one being transcriptionally active during the growth on benzoate. , 2013, Journal of environmental biology.

[19]  Jian Mao,et al.  Bioremediation of polycyclic aromatic hydrocarbon-contaminated soil by a bacterial consortium and associated microbial community changes , 2012 .

[20]  Howard A. Chase,et al.  Production of hydrogen and light hydrocarbons as a potential gaseous fuel from microwave-heated pyrolysis of waste automotive engine oil , 2012 .

[21]  A. Ayuba,et al.  Environmental Impacts of Oil Exploration and Exploitation in the Niger Delta of Nigeria , 2012 .

[22]  A. H. Zulkifly,et al.  Molecular identification and characterization of a bacterium that has potential to degrade low concentration of haloalkanoic acid , 2011 .

[23]  F. A. Adekola,et al.  Impacts of Simulated Agbabu Bitumen Leachate on Heamatological and Biochemical Parameters of Wistar Albino Rat , 2011 .

[24]  N. Vasudevan,et al.  Ortho and meta cleavage dioxygenases detected during the degradation of phenolic compounds by amoderately halophilic bacterial consortium , 2011 .

[25]  T. Narancic,et al.  Four Bacillus sp. soil isolates capable of degrading phenol, toluene, biphenyl, naphthalene and other aromatic compounds exhibit different aromatic catabolic potentials , 2011 .

[26]  K. Márialigeti,et al.  Investigation of catechol 2,3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater. , 2010, Systematic and applied microbiology.

[27]  P. R. M. Lopes,et al.  Microbial biodegradation potential of hydrocarbons evaluated by colorimetric technique: a case study , 2010 .

[28]  S. Burchiel,et al.  Some non-heterocyclic polycyclic aromatic hydrocarbons and some related exposures. , 2010, IARC monographs on the evaluation of carcinogenic risks to humans.

[29]  V. Prigione,et al.  Pyrene degradation and detoxification in soil by a consortium of basidiomycetes isolated from compost: role of laccases and peroxidases. , 2009, Journal of hazardous materials.

[30]  Alicja Szulc,et al.  Biodegradation and surfactant-mediated biodegradation of diesel fuel by 218 microbial consortia are not correlated to cell surface hydrophobicity , 2009, Applied Microbiology and Biotechnology.

[31]  M. Ilori,et al.  Degradation of hydrocarbons and biosurfactant production by Pseudomonas sp. strain LP1 , 2009 .

[32]  A. Etoumi,et al.  The reduction of wax precipitation in waxy crude oils by Pseudomonas species , 2008, Journal of Industrial Microbiology & Biotechnology.

[33]  A. R. Binupriya,et al.  Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas , 2008 .

[34]  D. Minai-Tehrani,et al.  Biodegradation of Aliphatic and Aromatic Fractions of Heavy Crude Oil–Contaminated Soil: A Pilot Study , 2007 .

[35]  A. Mukherjee,et al.  Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. , 2007, Bioresource technology.

[36]  H. Oh,et al.  Biodegradation of Aliphatic and Aromatic Hydrocarbons by Nocardia sp. H17-1 , 2006 .

[37]  Z. Shao,et al.  Pseudomonas, the dominant polycyclic aromatic hydrocarbon-degrading bacteria isolated from Antarctic soils and the role of large plasmids in horizontal gene transfer. , 2006, Environmental microbiology.

[38]  M. Ilori,et al.  Hydrocarbon Degrading Potentials of Bacteria Isolated from a Nigerian Bitumen (Tarsand) Deposit , 2006 .

[39]  Kirk T. Semple,et al.  Bioavailability of hydrophobic organic contaminants in soils: fundamental concepts and techniques for analysis , 2003 .

[40]  S. Sudhakar,et al.  Biodegradation of petroleum and crude oil by Pseudomonas putida and Bacillus cereus , 2002 .

[41]  F. Schinner,et al.  Bioremediation (Natural Attenuation and Biostimulation) of Diesel-Oil-Contaminated Soil in an Alpine Glacier Skiing Area , 2001, Applied and Environmental Microbiology.

[42]  R Amann,et al.  The identification of microorganisms by fluorescence in situ hybridisation. , 2001, Current opinion in biotechnology.

[43]  Margaret L. Britz,et al.  Degradation and Mineralization of High-Molecular-Weight Polycyclic Aromatic Hydrocarbons by Defined Fungal-Bacterial Cocultures , 2000, Applied and Environmental Microbiology.

[44]  Z. Zhou,et al.  Systematic Extensive Laboratory Studies of Microbial EOR Mechanisms and Microbial EOR Application Results in Changqing Oilfield , 1999 .

[45]  E. W. Jwanny,et al.  UTILIZATION OF HYDROCARBONS BY MICROORGANISMS , 1972 .