ISOLATION AND MOLECULAR CHARACTERIZATION OF PHYTASE PRODUCING BACTERIA

Potential phytase producing bacterial were isolated from which were Labis (Johor), Dusun Tua (Selangor), Ulu All bacterial samples were grown in Phytase Screening Media 37 °C, 45 ̊C, 50 ̊C and 55 C̊ for four days. Out of all samples tested, five isolates have showed clearing zone surrounding the colony wh were further analyzed in broth culture using sodium phytate as substrate. Among them, strain L3 was selected as the best producer (0.16 U/ml after 72 h of culture). This phytase showed optimal activity at 37 °C and pH5.5, demonstrating appropriate character for application in feed of several industrial processes. Blast homology of 16S rRNA gene sequence of isolate L3 identified 99% similarity to Acinetobacter baumannii.

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