The cell‐free fetal DNA fraction in maternal blood decreases after physical activity

If noninvasive prenatal testing using next generation sequencing is to be effective for pregnant women, a cell‐free fetal DNA (cffDNA) fraction above 4% is essential unless the depth of sequencing is increased. This study's objective is to determine whether physical activity has an effect on the proportion of cell‐free DNA (cfDNA) arising from the fetus (fetal fraction).

[1]  P. Jiang,et al.  High-resolution profiling of fetal DNA clearance from maternal plasma by massively parallel sequencing. , 2013, Clinical chemistry.

[2]  A. Paulussen,et al.  Noninvasive detection of fetal trisomy 21: systematic review and report of quality and outcomes of diagnostic accuracy studies performed between 1997 and 2012. , 2013, Human reproduction update.

[3]  A. Oliphant,et al.  Gestational age and maternal weight effects on fetal cell‐free DNA in maternal plasma , 2013, Prenatal diagnosis.

[4]  J. Canick,et al.  The impact of maternal plasma DNA fetal fraction on next generation sequencing tests for common fetal aneuploidies , 2013, Prenatal diagnosis.

[5]  K. Nicolaides,et al.  Fetal fraction in maternal plasma cell‐free DNA at 11–13 weeks' gestation: relation to maternal and fetal characteristics , 2013, Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology.

[6]  C. Lundby,et al.  Skeletal muscle vasodilatation during maximal exercise in health and disease , 2012, The Journal of physiology.

[7]  Jian Wang,et al.  Clinical application of massively parallel sequencing‐based prenatal noninvasive fetal trisomy test for trisomies 21 and 18 in 11 105 pregnancies with mixed risk factors , 2012, Prenatal diagnosis.

[8]  K. Nicolaides,et al.  Fetal Fraction in Maternal Plasma Cell-Free DNA at 11–13 Weeks’ Gestation: Effect of Maternal and Fetal Factors , 2012, Fetal Diagnosis and Therapy.

[9]  Arnold Oliphant,et al.  Noninvasive prenatal detection and selective analysis of cell-free DNA obtained from maternal blood: evaluation for trisomy 21 and trisomy 18. , 2012, American journal of obstetrics and gynecology.

[10]  S. Nelson,et al.  DNA sequencing of maternal plasma to detect Down syndrome: An international clinical validation study , 2011, Genetics in Medicine.

[11]  E. Dupont-Versteegden,et al.  Skeletal muscle apoptotic response to physical activity: potential mechanisms for protection. , 2011, Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme.

[12]  C. Cantor,et al.  Noninvasive detection of fetal trisomy 21 by sequencing of DNA in maternal blood: a study in a clinical setting. , 2011, American journal of obstetrics and gynecology.

[13]  H. C. Fan,et al.  Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood , 2008, Proceedings of the National Academy of Sciences.

[14]  W. Holzgreve,et al.  Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma. , 2005, Annals of the New York Academy of Sciences.

[15]  W. Holzgreve,et al.  Fluctuation of Maternal and Fetal Free Extracellular Circulatory DNA in Maternal Plasma , 2000, Obstetrics and gynecology.

[16]  T K Lau,et al.  Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. , 1998, American journal of human genetics.

[17]  Y. Lo,et al.  Rapid clearance of fetal DNA from maternal plasma. , 1999, American journal of human genetics.