Potential of newly isolated wild Streptomyces strains as agents for the biodegradation of a recalcitrant pharmaceutical, carbamazepine

Carbamazepine (CBZ) is a recalcitrant xenobiotic pharmaceutical pollutant highly stable in soil and wastewater during treatment. The biodegradation of CBZ using streptomycetes has been few studied up to now. Sixteen newly filamentous bacteria belong to genus Streptomyces spp. isolated from different Romanian soil samples and three strains from a collection of microorganisms (MIUG) were morphologically characterized, tested based on their resistance against CBZ toxicity and then selected as agents for bioremediation. Five Streptomyces spp. strains coded MIUG 4.88, MIUG 4.89, SNA, LP1 and LP2 showed CBZ tolerance at all of the tested concentrations, i.e. 0.05, 0.2, 1, 5 and 8 mg L−1. Two of these (MIUG 4.89 and SNA strains) were selected based on their resistance to target compound and were then assessed for CBZ biodegradation. The strain Streptomyces MIUG 4.89 showed an interesting efficiency for CBZ removal, with a yield of 35% when it was cultivated in submerged conditions on a minimal medium supplemented with 5 g L−1 glucose. This ability was linked to extracellular laccase production. These results are promising for the use of these filamentous bacteria as bioremediation agents.

[1]  N Kreuzinger,et al.  Carbamazepine as a possible anthropogenic marker in the aquatic environment: investigations on the behaviour of Carbamazepine in wastewater treatment and during groundwater infiltration. , 2004, Water research.

[2]  D. Dowling,et al.  Plant-Associated Bacterial Degradation of Toxic Organic Compounds in Soil , 2009, International journal of environmental research and public health.

[3]  Richard M. Dinsdale,et al.  The removal of pharmaceuticals, personal care products, endocrine disruptors and illicit drugs during wastewater treatment and its impact on the quality of receiving waters. , 2009, Water research.

[4]  Viviane Yargeau,et al.  Biodegradation of pharmaceuticals by Rhodococcus rhodochrous and Aspergillus niger by co-metabolism. , 2010, The Science of the total environment.

[5]  T. Nishida,et al.  Chemosphere Removal of estrogenic activities of bisphenol A and nonylphenol by oxidative enzymes from lignin-degrading basidiomycetes , 2009 .

[6]  A. Pollio,et al.  Ecotoxicological impact of pharmaceuticals found in treated wastewaters: study of carbamazepine, clofibric acid, and diclofenac. , 2003, Ecotoxicology and environmental safety.

[7]  A. Matuszewska,et al.  Fungal laccase: properties and activity on lignin , 2001, Journal of basic microbiology.

[8]  J. Bayona,et al.  Behavior of selected pharmaceuticals in subsurface flow constructed wetlands: a pilot-scale study. , 2005, Environmental science & technology.

[9]  G. Garrity.  Bergey’s Manual® of Systematic Bacteriology , 2012, Springer New York.

[10]  S. Lele,et al.  Enhanced production of laccase using a new isolate of white rot fungus WR-1 , 2006 .

[11]  P. Blánquez,et al.  Continuous biodegradation of 17beta-estradiol and 17alpha-ethynylestradiol by Trametes versicolor. , 2008, Journal of hazardous materials.

[12]  H. Okamura,et al.  Elimination of carbamazepine by repeated treatment with laccase in the presence of 1-hydroxybenzotriazole. , 2010, Journal of hazardous materials.

[13]  T. Vicent,et al.  Ability of white-rot fungi to remove selected pharmaceuticals and identification of degradation products of ibuprofen by Trametes versicolor. , 2009, Chemosphere.

[14]  T. Heberer.  Occurrence, fate, and removal of pharmaceutical residues in the aquatic environment: a review of recent research data. , 2002, Toxicology letters.

[15]  D. Schlosser,et al.  Degradation of the xenoestrogen nonylphenol by aquatic fungi and their laccases. , 2005, Microbiology.

[16]  K. Dhevendaran,et al.  Antagonistic Potential of Streptomyces Associated with the Gut of Marine Ornamental Fishes , 2011 .

[17]  A. Sartoratto,et al.  Degradation of the Recalcitrant Pharmaceuticals Carbamazepine and 17a-ethinylestradiol by Ligninolytic Fungi , 2012 .

[18]  Ji-ti Zhou,et al.  Isolation and characterization of Streptomyces spp. strains F-6 and F-7 capable of decomposing alkali lignin , 2012, Environmental technology.

[19]  A. Amrane,et al.  Bioaugmentation: Possible solution in the treatment of Bio-Refractory Organic Compounds (Bio-ROCs) , 2012 .

[20]  Y. Hadar,et al.  Transformation of the recalcitrant pharmaceutical compound carbamazepine by Pleurotus ostreatus: role of cytochrome P450 monooxygenase and manganese peroxidase. , 2011, Environmental science & technology.

[21]  Mira Petrovic,et al.  Analysis of pharmaceuticals in wastewater and removal using a membrane bioreactor , 2006, Analytical and bioanalytical chemistry.

[22]  H. Singer,et al.  Simultaneous quantification of neutral and acidic pharmaceuticals and pesticides at the low-ng/l level in surface and waste water. , 2001, Journal of chromatography. A.

[23]  Hahn,et al.  Analysis of bacterial communities in heavy metal-contaminated soils at different levels of resolution. , 1999, FEMS microbiology ecology.

[24]  C. S. Benimeli,et al.  Lindane uptake and degradation by aquatic Streptomyces sp. strain M7 , 2007 .

[25]  M. Suzuki,et al.  Polymerization of bisphenol A by purified laccase from Trametes villosa. , 2001, Biochemical and biophysical research communications.

[26]  J. Bollag,et al.  Microbial transformation of the herbicide metolachlor by a soil actinomycete , 1985 .

[27]  G. Feijoo,et al.  Oxidation of pharmaceutically active compounds by a ligninolytic fungal peroxidase , 2011, Biodegradation.

[28]  Y. Takashima,et al.  Degradation of bisphenol A by purified laccase from Trametes villosa. , 2001, Biochemical and biophysical research communications.

[29]  D. Barceló,et al.  Occurrence, partition and removal of pharmaceuticals in sewage water and sludge during wastewater treatment. , 2011, Water research.

[30]  Carla C. C. R. de Carvalho,et al.  Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes , 2011, Biodegradation.

[31]  Ronald M. Atlas,et al.  Handbook of microbiological media , 1993 .

[32]  D. Barceló,et al.  Removal of pharmaceuticals during wastewater treatment and environmental risk assessment using hazard indexes. , 2010, Environment international.

[33]  D. Hopwood.  Genetic analysis and genome structure in Streptomyces coelicolor , 1967, Bacteriological reviews.

[34]  Sven-Uwe Geissen,et al.  In vitro degradation of carbamazepine and diclofenac by crude lignin peroxidase. , 2010, Journal of hazardous materials.

[35]  N. Durán,et al.  Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review , 2000 .

[36]  C. Rensing,et al.  New Approaches for Bioaugmentation as a Remediation Technology , 2004 .

[37]  F. Kondo,et al.  Streptomyces sp. strain isolated from river water has high bisphenol A degradability , 2004, Letters in applied microbiology.

[38]  J. Santos,et al.  Occurrence, temporal evolution and risk assessment of pharmaceutically active compounds in Doñana Park (Spain). , 2010, Journal of hazardous materials.

[39]  H. Lechevalier.  A practical guide to generic identification of actinomycetes , 1989 .

[40]  I. Reid,et al.  Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization , 1995, Applied and environmental microbiology.

[41]  K. Kümmerer.  Pharmaceuticals in the Environment , 2001 .

[42]  G. Manfio,et al.  Biodegradation of the herbicide Diuron in soil by indigenous actinomycetes. , 1998, Chemosphere.

[43]  C. S. Benimeli,et al.  Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides. , 2003, Bioresource technology.

[44]  R. Tyagi,et al.  Laccases for removal of recalcitrant and emerging pollutants. , 2010, Bioresource technology.

[45]  J. Sohng,et al.  Cytochrome P450 (CYP105F2) from Streptomyces peucetius and its activity with oleandomycin , 2008, Applied Microbiology and Biotechnology.

[46]  K. Schleifer,et al.  The family Streptomycetaceae , 1981 .

[47]  J N Lester,et al.  Human pharmaceuticals in the aquatic environment a review. , 2001, Environmental technology.

[48]  Frank Sacher,et al.  Removal of pharmaceuticals during drinking water treatment. , 2002, Environmental science & technology.

[49]  V. Yargeau,et al.  Removal of Aqueous 17α-Ethinylestradiol by Rhodococcus Species , 2009 .