The phenomenon of fish farmers in reducing the high growth of bacteria in fish ponds of freshwater by using salt (krosok), but not all the bacteria killed (especially Pseudomonas aeruginosa). This objective research is proving to increase some knob counts (small ball) as ultrasonic transducer, there is a linier increase in the forward intensity of ultrasonic and an exponensial increase the death of Pseudomonas aeruginosa colony. Pseudomonas aeruginosa (ATCC 27853) can be grown in a nutrient agar, a nutrient broth, a human blood agar plate, a koi blood plate. In this research especially diluted media in water of physiology as compared and control for viability of Pseudomonas aeruginosa. In the media, these bacteria exposed ultrasonic for 30 minutes. The exposure system of ultrasonic uses some knob (ie: 3, 6, 9, 12 knops) and the media would have own optimum frequency in the increased intensity (energy) and the increased death of Pseudomonas aeruginosa, which depends on the homogeneity when it created the media of Pseudomonas aeruginosa. This study shows each medium has own optimum frequency, each some knob of ultrasonic transmitter, has the intensity transmitte increase of ultrasonic and in the death of Pseudomonas aeruginosa colony. Application of ultrasonic exposure is new knowledge related to energy ultrasonic and the ability to pass on skills lethal of Pseudomonas aeruginosa colony related differensis knops counts are derived from the same source intensity value.