Effect of vincristine on sister chromatid exchanges of normal human lymphocytes.

In vitro analysis of the mitosis from 3-day lymphocyte cultures from two normal males and two normal females treated with bromodeoxyuridine and stained with acridine orange after addition of vincristine during different times demonstrated that vincristine inhibits the progression of the second division. When the second division progressed, the number of sister chromatid exchanges was significantly decreased. Vincristine could act by inhibiting the copying of DNA. A dosage effect is demonstrated when vincristine is added to the cultures during the first 24 hr.

[1]  H. Kato.  Possible role of DNA synthesis in formation of sister chromatid exchanges , 1974, Nature.

[2]  H. Kato.  Spontaneous sister chromatid exchanges detected by a BUdR-labelling method , 1974, Nature.

[3]  S. Wolff,et al.  Sister chromatid exchanges induced by light flashes to 5-bromodeoxyuridine- and 5-iododeoxyuridine substituted Chinese hamster chromosomes. , 1974, Experimental cell research.

[4]  B. Dutrillaux,et al.  Analyse des changes de chromatides dans les cellules somatiques humaines: Traitement au BUDR (5 bromodoxyuridine) et fluorescence bicolore par l'acridine orange , 1974 .

[5]  H. Kato.  Induction of sister chromatid exchanges by UV light and its inhibition by caffeine. , 1973, Experimental cell research.

[6]  S. Latt.  Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. Cardinali,et al.  The Catharanthus roseus (Vinca rosea) alkaloids: a new class of stathmokinetic agents. , 1968, Acta geneticae medicae et gemellologiae.

[8]  P. Dustin.  Some observations on the cytological effects of antimitotic poisons. , 1968, Acta geneticae medicae et gemellologiae.

[9]  P. George,et al.  Effect of vincristine on the fine structure of HeLa cells during mitosis. , 1965, Journal of the National Cancer Institute.

[10]  D. Rall,et al.  The Stathmokinetic Effect of Vincristine , 1964 .

[11]  W. Creasey,et al.  BIOCHEMICAL EFFECTS OF THE VINCA ALKALOIDS. II. A COMPARISON OF THE EFFECTS OF COLCHICINE, VINBLASTINE AND VINCRISTINE ON THE SYNTHESIS OF RIBONUCLEIC ACIDS IN EHRLICH ASCITES CARCINOMA CELLS. , 1964, Biochimica et biophysica acta.

[12]  G. Cardinali,et al.  Studies on the Antimitotic Activity of Leurocristine (Vincristine) , 1963 .