Laser-induced priming of human blood leukocytes

We investigated the influence of He-Ne ((lambda) equals 632.8 nm) laser irradiation (LI) on a functional activity of human blood leucocytes. The method of luminol-dependent chemiluminescence with the zymosan-activated phagocytes was used. The leucocytes were irradiated without and in the presence of autologic human blood plasma, containing of the endogenous (porphyrins) and/or exogenous (phthalocyanine) photosensitizers. The LI initiated a priming of the leucocytes. Priming revealed itself after the activation of the phagocytes by zymosan. The changes of the calcium concentration in leucocytes cytoplasm were studied too. Fluorimetric method with Fura-2AM was used for this. The laser irradiation initiated the changes of the calcium concentration in the leucocytes cytoplasm. All the investigating parameters depended on the irradiation dose and on the concentration of photosensitizers. The results of this work allowed to formulate the main theses of the free radical mechanism of the low intensive laser irradiation action on human blood leucocytes.

[1]  G. I. Klebanov,et al.  Effect of oxidized phospholipids on the chemiluminescence of zymosan-activated leukocytes. , 1990, General physiology and biophysics.

[2]  J. Poderoso,et al.  Nitric oxide synthase inhibitors decrease human polymorphonuclear leukocyte luminol-dependent chemiluminescence. , 1995, Free radical biology & medicine.

[3]  T. Karu,et al.  Photobiology of low-power laser effects. , 1989, Health physics.

[4]  R Y Tsien,et al.  Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator , 1982, The Journal of cell biology.

[5]  W. Phillips,et al.  Priming of neutrophils and macrophages for enhanced release of superoxide anion by the calcium ionophore ionomycin. Implications for regulation of the respiratory burst. , 1987, The Journal of biological chemistry.

[6]  Zvi Malik,et al.  A POSSIBLE MECHANISM OF LOW LEVEL LASER-LIVING CELL INTERACTION , 1990 .

[7]  S Passarella,et al.  Increase in <--H+/e- ratio of the cytochrome c oxidase reaction in mitochondria irradiated with helium-neon laser. , 1994, Biochemistry and molecular biology international.

[8]  I Rosenthal,et al.  PHTHALOCYANINES AS PHOTODYNAMIC SENSITIZERS * , 1991, Photochemistry and photobiology.

[9]  L. Mcphail,et al.  Exposure of human neutrophils to chemotactic factors potentiates activation of the respiratory burst enzyme. , 1983, Journal of Immunology.

[10]  S. Snyder,et al.  Nitric oxide, a novel biologic messenger , 1992, Cell.