Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation.

The Xa1 gene in rice confers resistance to Japanese race 1 of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial blight (BB). We isolated the Xa1 gene by a map-based cloning strategy. The deduced amino acid sequence of the Xa1 gene product contains nucleotide binding sites (NBS) and a new type of leucine-rich repeats (LRR); thus, Xa1 is a member of the NBS-LRR class of plant disease-resistance genes, but quite different from Xa21, another BB-resistance gene isolated from rice. Interestingly, Xa1 gene expression was induced on inoculation with a bacterial pathogen and wound, unlike other isolated resistance genes in plants, which show constitutive expression. The induced expression may be involved in enhancement of resistance against the pathogen.

[1]  J. Luck,et al.  Advances in the molecular genetic analysis of the flax-flax rust interaction. , 1997, Annual review of phytopathology.

[2]  E. A. van der Biezen,et al.  The Arabidopsis downy mildew resistance gene RPP5 shares similarity to the toll and interleukin-1 receptors with N and L6. , 1997, The Plant cell.

[3]  F. Ausubel,et al.  The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats , 1994, Cell.

[4]  W. F. Thompson,et al.  Rapid isolation of high molecular weight plant DNA. , 1980, Nucleic acids research.

[5]  Li-li Chen,et al.  A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21 , 1995, Science.

[6]  G. Martin,et al.  Map-based cloning of a protein kinase gene conferring disease resistance in tomato. , 1993, Science.

[7]  P. Quail,et al.  Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants. , 1992, Plant physiology.

[8]  W. Stiekema,et al.  Positional Cloning of a Gene for Nematode Resistance in Sugar Beet , 1997, Science.

[9]  E. Finnegan,et al.  The L6 gene for flax rust resistance is related to the Arabidopsis bacterial resistance gene RPS2 and the tobacco viral resistance gene N. , 1995, The Plant cell.

[10]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[11]  Jonathan D. G. Jones,et al.  The Tomato Cf-2 Disease Resistance Locus Comprises Two Functional Genes Encoding Leucine-Rich Repeat Proteins , 1996, Cell.

[12]  S. Dinesh-Kumar,et al.  Signaling in plant-microbe interactions. , 1997, Science.

[13]  S. Tanksley,et al.  The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes. , 1997, The Plant cell.

[14]  J. Dangl,et al.  Plant pathology: Many roads lead to resistance , 1996, Current Biology.

[15]  J. Dangl,et al.  Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance , 1995, Science.

[16]  J. D. Jones,et al.  Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. , 1994, Science.

[17]  T. Mew.  CURRENT STATUS AND FUTURE PROSPECTS OF RESEARCH ON BACTERIAL BLIGHT OF RICE , 1987 .

[18]  G. Churchill,et al.  Pooled-sampling makes high-resolution mapping practical with DNA markers. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[19]  E. Finnegan,et al.  Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region. , 1997, The Plant cell.

[20]  Jonathan D. G. Jones.  A kinase with keen eyes , 1997, Nature.

[21]  J. Giraudat,et al.  RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. , 1994, Science.

[22]  S. Dinesh-Kumar,et al.  The product of the tobacco mosaic virus resistance gene N: Similarity to toll and the interleukin-1 receptor , 1994, Cell.