Modular Synthase-Encoding Gene Involved in α-Olefin Biosynthesis in Synechococcus sp. Strain PCC 7002

ABSTRACT A gene involved in the production of medium-chain α-olefins was identified in the cyanobacterium Synechococcus sp. strain PCC 7002. The gene encodes a large multidomain protein with homology to type I polyketide synthases, suggesting a route for hydrocarbon biosynthesis from fatty acids via an elongation decarboxylation mechanism.

[1]  J. Keasling,et al.  Genes Involved in Long-Chain Alkene Biosynthesis in Micrococcus luteus , 2009, Applied and Environmental Microbiology.

[2]  P. L. Parker,et al.  Fatty Acids in Eleven Species of Blue-Green Algae: Geochemical Significance , 1967, Science.

[3]  Bo Wang,et al.  Polyketide decarboxylative chain termination preceded by o-sulfonation in curacin a biosynthesis. , 2009, Journal of the American Chemical Society.

[4]  Martin Fulda,et al.  Fatty Acid Activation in Cyanobacteria Mediated by Acyl-Acyl Carrier Protein Synthetase Enables Fatty Acid Recycling1[W] , 2010, Plant Physiology.

[5]  C. Largeau,et al.  Biosynthesis of non-isoprenoid hydrocarbons by the microalga Botryococcus braunii. Evidences for elongation-decarboxylation mechanism. Activation of decarboxylation , 1986 .

[6]  P. L. Parker,et al.  Hydrocarbons of Blue-Green Algae: Geochemical Signfficance , 1969, Science.

[7]  S. Donadio,et al.  Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. , 1992, Gene.

[8]  Brian F Pfleger,et al.  A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes , 2010, Biotechnology and bioengineering.

[9]  P. Leadlay,et al.  Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl‐CoA:acyl carrier protein transacylase domains in modular polyketide synthases , 1995, FEBS letters.

[10]  P. Black,et al.  Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase. , 1992, The Journal of biological chemistry.

[11]  A. Schirmer,et al.  Microbial Biosynthesis of Alkanes , 2010, Science.

[12]  D. Bryant,et al.  Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation. , 2004, Methods in molecular biology.

[13]  Nigel S. Scrutton,et al.  Redesign of the coenzyme specificity of a dehydrogenase by protein engineering , 1990, Nature.

[14]  P. Lindblad,et al.  Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria , 2009, BMC Molecular Biology.

[15]  J. Myers,et al.  THE PRODUCTION OF HYDROGEN PEROXIDE BY BLUE‐GREEN ALGAE: A SURVEY 1 , 1973 .

[16]  R. Light,et al.  Structure and composition of hydrocarbons and fatty acids from a marine blue-green alga, Synechococcus sp. , 1982 .

[17]  J. Elhai.  Strong and regulated promoters in the cyanobacterium Anabaena PCC 7120. , 1993, FEMS microbiology letters.

[18]  P. Leadlay,et al.  Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase. , 1996, Gene.

[19]  Andreas Schirmer,et al.  Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species , 2011, Applied and Environmental Microbiology.

[20]  David H Sherman,et al.  Biosynthetic pathway and gene cluster analysis of curacin A, an antitubulin natural product from the tropical marine cyanobacterium Lyngbya majuscula. , 2004, Journal of natural products.

[21]  G. Cassani,et al.  Determination of double-bond position in diunsaturated compounds by mass spectrometry of dimethyl disulfide derivatives , 1987 .