Established IL‐2‐dependent double‐negative (CD4‐ CD8‐) TCRαβ/CD3+ ATL cells: induction of CD4 expression

Summary. We established IL‐2‐dependent T cells from an adult T‐cell leukaemia (ATL) patient whose leukaemic cells changed from CD4 single‐p‐positive in the initial phase to double‐negative (CD4‐ CD8‐) at the time of exacerbation. The cells termed SO‐4 were of ATL cell origin and showed the double‐negative TCRαβ/CD3+ T‐cell phenotype. SO‐4 cells acquired CD4 antigen expression following stimulation with concanavlin A (ConA) or immobilized anti‐CD3 antibody. The induction was inhibited by herbimycin A, an inhibitor of protein tyrosine kinase (PTK) activity. No CD4 mRNA was detectable in unstimulated SO‐4 cells but a 3.0 kb signal specific for CD4 mRNA was detected after stimulation. These findings indicate that SO‐4 cells return to their original phenotype (CD4 single‐positive) by stimulation involving PTK. The results indicate that there is a pathway of phenotypic cycling between CD4 single‐positive and double‐negative T cells.

[1]  A. Vicari,et al.  A novel CD45RA+CD4+ transient thymic subpopulation in MRL-lpr/lpr mice: its relation to non-proliferating CD4-CD8-CD45RA+ tumor cells. , 1993, International immunology.

[2]  M. Tomonaga,et al.  DISAPPEARANCE OF CD4 ANTIGEN IN A CASE OF ADULT T CELL LEUKAEMIA , 1992, British journal of haematology.

[3]  P. Casali,et al.  Model for studying virus attachment. II. Binding of biotinylated human T cell leukemia virus type I to human blood mononuclear cells potential targets for human T cell leukemia virus type I infection. , 1991, Journal of immunology.

[4]  F. Lori,et al.  Induction of CD4 and susceptibility to HIV-1 infection in human CD8+ T lymphocytes by human herpesvirus 6 , 1991, Nature.

[5]  M. Tomonaga,et al.  IL-2-dependent ATL cell lines with phenotypes differing from the original leukemia cells. , 1991, Leukemia research.

[6]  G. Schieven,et al.  Inhibition of tyrosine phosphorylation prevents T-cell receptor-mediated signal transduction. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[7]  T. Abo,et al.  Liver is a possible site for the proliferation of abnormal CD3+4-8- double-negative lymphocytes in autoimmune MRL-lpr/lpr mice , 1990, The Journal of experimental medicine.

[8]  T. Mustelin,et al.  T cell antigen receptor-mediated activation of phospholipase C requires tyrosine phosphorylation. , 1990, Science.

[9]  C. June,et al.  Increases in tyrosine phosphorylation are detectable before phospholipase C activation after T cell receptor stimulation. , 1990, Journal of immunology.

[10]  M. Feldmann,et al.  Definition of a population of CD4-8- T cells that express the alpha beta T-cell receptor and respond to interleukins 2, 3, and 4. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[11]  J. Strominger,et al.  Double-negative (CD4-CD8-) lymphocytes bearing T-cell receptor alpha and beta chains in normal human skin. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[12]  G. Tsokos,et al.  T cell receptor alpha/beta expressing double-negative (CD4-/CD8-) and CD4+ T helper cells in humans augment the production of pathogenic anti-DNA autoantibodies associated with lupus nephritis. , 1989, Journal of immunology.

[13]  Yasuaki Yamada,et al.  Adult T cell leukaemia cells are of CD4+ CDw29+ T cell origin and secrete a B cell differentiation factor , 1989, British journal of haematology.

[14]  P. Marrack,et al.  Demethylated CD8 gene in CD4+ T cells suggests that CD4+ cells develop from CD8+ precursors. , 1988, Science.

[15]  H. Spits,et al.  Interleukin-4 mediates CDS induction on human CD4+ T-cell clones , 1988, Nature.

[16]  J. Hoxie,et al.  T4 endocytosis and phosphorylation induced by phorbol esters but not by mitogen or HIV infection. , 1988, Journal of immunology.

[17]  R. Amson,et al.  Internalization of human T lymphocyte receptors , 1987, European journal of immunology.

[18]  B. Gallis,et al.  Rapid phosphorylation and modulation of the T4 antigen on cloned helper T cells induced by phorbol myristate acetate or antigen. , 1986, The Journal of biological chemistry.

[19]  P. M. Chisholm,et al.  Co-expression of CD4 and CD8 molecules and de novo expression of MHC class II antigens on activated rat T cells. , 1986, Immunology.

[20]  J. Hudson,et al.  Effect of an inhibitor of DNA methylation on T cells. I. 5-Azacytidine induces T4 expression on T8+ T cells. , 1986, Journal of immunology.

[21]  S. Kamihira,et al.  Adult T cell leukemia with atypical surface phenotypes: clinical correlation. , 1985, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[22]  J. Daley,et al.  Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry. , 1985, Journal of immunology.

[23]  F. Reyes,et al.  A subset of OKT4+ peripheral T cells can generate colonies containing mixed progeny with OKT4+ helper and OKT8+ suppressor cells , 1985, European journal of immunology.

[24]  S. O’Brien,et al.  Isolation of HTLV-transformed B-lymphocyte clone from a patient with HTLV-associated adult T-cell leukaemia , 1984, Nature.

[25]  K. Yamaguchi,et al.  Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[26]  S. Kamihira,et al.  Changes of adult T cell leukemia cell surface antigens at relapse or at exacerbation phase after chemotherapy defined by use of monoclonal antibodies. , 1984, Blood.

[27]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[28]  Y. Yamada.  Phenotypic and functional analysis of leukemic cells from 16 patients with adult T-cell leukemia/lymphoma. , 1983, Blood.

[29]  C. B. Henning,et al.  Differentiation of human T-lymphoid leukemia cells into cells that have a suppressor phenotype is induced by phorbol 12-myristate 13-acetate. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[30]  W. Solbach.  Brief Definitive Report TUMOR-PROMOTING PHORBOL ESTERS SELECTIVELY ABROGATE THE EXPRESSION OF THE T4 DIFFERENTIATION ANTIGEN EXPRESSED ON NORMAL AND MALIGNANT (SI),ZARY) T HELPER LYMPHOCYTES* , 2003 .

[31]  K. Nagata,et al.  Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[32]  E. Reinherz,et al.  A rapid method for separating functionally intact human T lymphocytes with monoclonal antibodies. , 1981, Clinical immunology and immunopathology.

[33]  John D. Minna,et al.  Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma , 1980, Proceedings of the National Academy of Sciences.

[34]  J. Yodoi,et al.  Adult T-cell leukemia: clinical and hematologic features of 16 cases. , 1977, Blood.

[35]  H. Towler,et al.  Adult T-cell leukemia : antigen in an ATL cell line and detection of antibodies to the antigen in human sera , 2022 .