Brillouin micro-spectroscopy through aberrations via sensorless adaptive optics

Brillouin spectroscopy is a powerful optical technique for non-contact viscoelastic characterizations which has recently found applications in three-dimensional mapping of biological samples. Brillouin spectroscopy performances are rapidly degraded by optical aberrations and have therefore been limited to homogenous transparent samples. In this work, we developed an adaptive optics (AO) configuration designed for Brillouin scattering spectroscopy to engineer the incident wavefront and correct for aberrations. Our configuration does not require direct wavefront sensing and the injection of a “guide-star”; hence, it can be implemented without the need for sample pre-treatment. We used our AO-Brillouin spectrometer in aberrated phantoms and biological samples and obtained improved precision and resolution of Brillouin spectral analysis; we demonstrated 2.5-fold enhancement in Brillouin signal strength and 1.4-fold improvement in axial resolution because of the correction of optical aberrations.

[1]  Carl Paterson,et al.  Elastic suppression in Brillouin imaging by destructive interference , 2015 .

[2]  T. Wilson,et al.  Adaptive aberration correction in a confocal microscope , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[3]  D. Gavel,et al.  Adaptive optics wide-field microscopy using direct wavefront sensing. , 2011, Optics letters.

[4]  Martin J. Booth,et al.  Adaptive optical microscopy: the ongoing quest for a perfect image , 2014, Light: Science & Applications.

[5]  Vladislav V. Yakovlev,et al.  Impulsive Brillouin microscopy , 2017 .

[6]  G. Benedek,et al.  Brillouin Scattering in Cubic Crystals , 1966 .

[7]  Giuliano Scarcelli,et al.  High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media , 2016, Applied physics letters.

[8]  Itay Remer,et al.  Background-free Brillouin spectroscopy in scattering media at 780  nm via stimulated Brillouin scattering. , 2016, Optics letters.

[9]  Takashi R Sato,et al.  Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex , 2011, Proceedings of the National Academy of Sciences.

[10]  P. Artal,et al.  Adaptive-optics ultrahigh-resolution optical coherence tomography. , 2004, Optics letters.

[11]  S. Yun,et al.  In vivo biomechanical mapping of normal and keratoconus corneas. , 2015, JAMA ophthalmology.

[12]  Rob Krams,et al.  Quantification of plaque stiffness by Brillouin microscopy in experimental thin cap fibroatheroma , 2015, Journal of The Royal Society Interface.

[13]  Giuliano Scarcelli,et al.  Etalon filters for Brillouin microscopy of highly scattering tissues. , 2016, Optics express.

[14]  William J. Polacheck,et al.  Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy , 2015, Nature Methods.

[15]  Brandon K. Harvey,et al.  Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue , 2015, Nature Communications.

[16]  Kristie J. Koski,et al.  Non-invasive determination of the complete elastic moduli of spider silks. , 2013, Nature materials.

[17]  S. Yun,et al.  Multistage VIPA etalons for high-extinction parallel Brillouin spectroscopy , 2011, Optics express.

[18]  Giuliano Scarcelli,et al.  Brillouin flow cytometry for label-free mechanical phenotyping of the nucleus. , 2017, Lab on a chip.

[19]  Giuliano Scarcelli,et al.  In Vivo Brillouin Analysis of the Aging Crystalline Lens , 2016, Investigative ophthalmology & visual science.

[20]  G. Scarcelli,et al.  Improving localization precision of Brillouin measurements using spectral autocorrelation analysis , 2017 .

[21]  C. Soles,et al.  Acoustic modes and elastic properties of polymeric nanostructures , 2005 .

[22]  David Williams,et al.  The arrangement of the three cone classes in the living human eye , 1999, Nature.

[23]  Vladislav V. Yakovlev,et al.  Background clean-up in Brillouin microspectroscopy of scattering medium. , 2014, Optics express.

[24]  D. Milkie,et al.  Rapid Adaptive Optical Recovery of Optimal Resolution over LargeVolumes , 2014, Nature Methods.

[25]  Martin J. Booth,et al.  Adaptive optics in microscopy , 2003, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[26]  X. Xie,et al.  Adaptive optics for enhanced signal in CARS microscopy. , 2007, Optics express.

[27]  Tony Wilson,et al.  Image-based adaptive optics for two-photon microscopy. , 2009, Optics letters.

[28]  Eric Betzig,et al.  Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues , 2010, Nature Methods.

[29]  Na Ji,et al.  Multiplexed aberration measurement for deep tissue imaging in vivo , 2014, Nature Methods.

[30]  Raphaël Turcotte,et al.  Adaptive optical versus spherical aberration corrections for in vivo brain imaging. , 2017, Biomedical optics express.

[31]  Kareem Elsayad,et al.  Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging , 2016, Science Signaling.

[32]  Giuliano Scarcelli,et al.  Integration of spectral coronagraphy within VIPA-based spectrometers for high extinction Brillouin imaging. , 2017, Optics express.

[33]  Giuseppe Antonacci,et al.  Biomechanics of subcellular structures by non-invasive Brillouin microscopy , 2016, Scientific Reports.

[34]  Giuliano Scarcelli,et al.  Line-scanning Brillouin microscopy for rapid non-invasive mechanical imaging , 2016, Scientific Reports.

[35]  Silvia Caponi,et al.  Biomechanics of fibrous proteins of the extracellular matrix studied by Brillouin scattering , 2014, Journal of The Royal Society Interface.

[36]  M. Booth.  Adaptive optics in microscopy. , 2003, Philosophical transactions. Series A, Mathematical, physical, and engineering sciences.

[37]  R. Boyd.  The Nonlinear Optical Susceptibility , 2020, Nonlinear Optics.

[38]  J. Dil.  Brillouin scattering in condensed matter , 1982 .

[39]  Thomas G Bifano,et al.  Adaptive optics in multiphoton microscopy: comparison of two, three and four photon fluorescence. , 2015, Optics express.

[40]  S. Yun,et al.  Confocal Brillouin microscopy for three-dimensional mechanical imaging. , 2007, Nature photonics.

[41]  Gregory W. Faris,et al.  High-resolution stimulated Brillouin gain spectroscopy in glasses and crystals , 1993 .

[42]  Steven M. Jones,et al.  Adaptive-optics optical coherence tomography for high-resolution and high-speed 3 D retinal in vivo imaging , 2005 .

[43]  T. Hebert,et al.  Adaptive optics scanning laser ophthalmoscopy. , 2002, Optics express.

[44]  Jordi Andilla,et al.  Deep and Clear Optical Imaging of Thick Inhomogeneous Samples , 2012, PloS one.

[45]  D. Fioretto,et al.  High-contrast Brillouin and Raman micro-spectroscopy for simultaneous mechanical and chemical investigation of microbial biofilms. , 2017, Biophysical chemistry.

[46]  Jerome Mertz,et al.  Conjugate adaptive optics in widefield microscopy with an extended-source wavefront sensor , 2015, 1506.03463.

[47]  J. Boon,et al.  Brillouin Scattering in Simple Liquids: Argon and Neon , 1969 .

[48]  Jerome Mertz,et al.  Field of view advantage of conjugate adaptive optics in microscopy applications. , 2015, Applied optics.

[49]  Xiaodong Tao,et al.  Adaptive optics confocal microscopy using direct wavefront sensing. , 2011, Optics letters.

[50]  T. Wilson,et al.  Aberration correction for confocal imaging in refractive‐index‐mismatched media , 1998 .

[51]  Thomas D. Wang,et al.  Miniature near-infrared dual-axes confocal microscope utilizing a two-dimensional microelectromechanical systems scanner. , 2007, Optics letters.

[52]  Vladislav V. Yakovlev,et al.  Seeing cells in a new light: a renaissance of Brillouin spectroscopy , 2016 .

[53]  Na Ji.  Adaptive optical fluorescence microscopy , 2017, Nature Methods.