Phenotypic landscape of granulocytes and monocytes by multiparametric flow cytometry: A prospective study of a 1‐tube panel strategy for diagnosis and prognosis of patients with MDS

Multiparametric flow cytometry (MFC) was recently reported to be a helpful additional tool in the diagnosis of myelodysplastic syndromes (MDS). However, numerous aberrancies have been reported that makes their evaluation difficult as part of a routine diagnosis.

[1]  A. A. van de Loosdrecht,et al.  Clinical Implication of Multi-Parameter Flow Cytometry in Myelodysplastic Syndromes , 2018, Pathobiology.

[2]  B. Ebert,et al.  Proposed minimal diagnostic criteria for myelodysplastic syndromes (MDS) and potential pre-MDS conditions , 2017, Oncotarget.

[3]  A. Órfão,et al.  Immunophenotypic analysis of erythroid dysplasia in myelodysplastic syndromes. A report from the IMDSFlow working group , 2017, Haematologica.

[4]  G. Ossenkoppele,et al.  The myelodysplastic syndromes flow cytometric score: a three-parameter prognostic flow cytometric scoring system , 2016, Leukemia.

[5]  F Lacombe,et al.  Harmonemia: a universal strategy for flow cytometry immunophenotyping—A European LeukemiaNet WP10 study , 2016, Leukemia.

[6]  P. M. van de Ven,et al.  Regulatory T cells and progenitor B cells are independent prognostic predictors in lower risk myelodysplastic syndromes , 2015, Haematologica.

[7]  T. Haferlach,et al.  Multiparameter flow cytometry provides independent prognostic information in patients with suspected myelodysplastic syndromes: A study on 804 patients , 2015, Cytometry. Part B, Clinical cytometry.

[8]  M. Béné,et al.  Multicentric study underlining the interest of adding CD5, CD7 and CD56 expression assessment to the flow cytometric Ogata score in myelodysplastic syndromes and myelodysplastic/myeloproliferative neoplasms , 2015, Haematologica.

[9]  M. Loken,et al.  Assessment of erythroid dysplasia by “Difference from normal” in routine clinical flow cytometry workup , 2014, Cytometry. Part B, Clinical cytometry.

[10]  G. Ossenkoppele,et al.  High flow cytometric scores identify adverse prognostic subgroups within the revised international prognostic scoring system for myelodysplastic syndromes , 2014, British journal of haematology.

[11]  A Orfao,et al.  Revisiting guidelines for integration of flow cytometry results in the WHO classification of myelodysplastic syndromes—proposal from the International/European LeukemiaNet Working Group for Flow Cytometry in MDS , 2014, Leukemia.

[12]  F. Lacombe,et al.  Comparable flow cytometry data can be obtained with two types of instruments, Canto II, and Navios. A GEIL study , 2013, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[13]  M. Cazzola,et al.  Diagnosis and treatment of primary myelodysplastic syndromes in adults: recommendations from the European LeukemiaNet. , 2013, Blood.

[14]  M. Béné,et al.  Flow cytometric detection of dyserythropoiesis: a sensitive and powerful diagnostic tool for myelodysplastic syndromes , 2013, Leukemia.

[15]  Luca Malcovati,et al.  Revised international prognostic scoring system for myelodysplastic syndromes. , 2012, Blood.

[16]  C. Pascutto,et al.  Multicenter validation of a reproducible flow cytometric score for the diagnosis of low-grade myelodysplastic syndromes: results of a European LeukemiaNET study , 2012, Haematologica.

[17]  A. Órfão,et al.  Standardization of flow cytometry in myelodysplastic syndromes: a report from an international consortium and the European LeukemiaNet Working Group , 2012, Leukemia.

[18]  M. Loken,et al.  Flow cytometric scoring system as a diagnostic and prognostic tool in myelodysplastic syndromes. , 2011, Leukemia research.

[19]  J. Hernández-Rivas,et al.  Bone marrow cells from myelodysplastic syndromes show altered immunophenotypic profiles that may contribute to the diagnosis and prognostic stratification of the disease: A pilot study on a series of 56 patients , 2010, Cytometry. Part B, Clinical cytometry.

[20]  L. Malcovati,et al.  Diagnostic utility of flow cytometry in low-grade myelodysplastic syndromes: a prospective validation study , 2009, Haematologica.

[21]  A. Órfão,et al.  Flow cytometry in myelodysplastic syndromes: report from a working conference. , 2008, Leukemia research.

[22]  H. Deeg,et al.  Myeloid and monocytic dyspoiesis as determined by flow cytometric scoring in myelodysplastic syndrome correlates with the IPSS and with outcome after hematopoietic stem cell transplantation. , 2003, Blood.

[23]  M. Mori,et al.  Clinical significance of phenotypic features of blasts in patients with myelodysplastic syndrome. , 2002, Blood.

[24]  C. Haanen,et al.  A method for quantification of peripheral blood admixture in bone marrow aspirates. , 1980, Experimental hematology.