Pulling on the nascent RNA during transcription does not alter kinetics of elongation or ubiquitous pausing.

[1]  Steven M. Block,et al.  Sequence-Resolved Detection of Pausing by Single RNA Polymerase Molecules , 2006, Cell.

[2]  Tao Pan,et al.  RNA folding during transcription. , 2006, Annual review of biophysics and biomolecular structure.

[3]  D. Herschlag,et al.  Nanomechanical measurements of the sequence-dependent folding landscapes of single nucleic acid hairpins. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Jeffrey W. Roberts,et al.  Role of DNA bubble rewinding in enzymatic transcription termination. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[5]  H. Gaub,et al.  Dynamic force spectroscopy of the digoxigenin–antibody complex , 2006, FEBS letters.

[6]  W. Greenleaf,et al.  Direct observation of base-pair stepping by RNA polymerase , 2005, Nature.

[7]  Satoru Miyano,et al.  Prediction of Transcriptional Terminators in Bacillus subtilis and Related Species , 2005, PLoS Comput. Biol..

[8]  K. Neuman,et al.  Measurement of the effective focal shift in an optical trap. , 2005, Optics letters.

[9]  Arkady Mustaev,et al.  A Ratchet Mechanism of Transcription Elongation and Its Control , 2005, Cell.

[10]  Polly M Fordyce,et al.  Simultaneous, coincident optical trapping and single-molecule fluorescence , 2004, Nature Methods.

[11]  Yeonee Seol,et al.  Elastic properties of a single-stranded charged homopolymeric ribonucleotide. , 2004, Physical review letters.

[12]  Michelle D. Wang,et al.  Molecular mechanism of transcription inhibition by peptide antibiotic Microcin J25. , 2004, Molecular cell.

[13]  Jeffrey W. Roberts,et al.  Forward translocation is the natural pathway of RNA release at an intrinsic terminator. , 2004, Molecular cell.

[14]  R. Landick Active-Site Dynamics in RNA Polymerases , 2004, Cell.

[15]  Robert Landick,et al.  Diversity in the Rates of Transcript Elongation by Single RNA Polymerase Molecules* , 2004, Journal of Biological Chemistry.

[16]  Joshua W. Shaevitz,et al.  Backtracking by single RNA polymerase molecules observed at near-base-pair resolution , 2003, Nature.

[17]  Elio A. Abbondanzieri,et al.  Ubiquitous Transcriptional Pausing Is Independent of RNA Polymerase Backtracking , 2003, Cell.

[18]  Robert Landick,et al.  The flap domain is required for pause RNA hairpin inhibition of catalysis by RNA polymerase and can modulate intrinsic termination. , 2003, Molecular cell.

[19]  Jean-François Léger,et al.  Probing complex RNA structures by mechanical force , 2003, The European physical journal. E, Soft matter.

[20]  Michael Zuker,et al.  Mfold web server for nucleic acid folding and hybridization prediction , 2003, Nucleic Acids Res..

[21]  Ignacio Tinoco,et al.  Identifying Kinetic Barriers to Mechanical Unfolding of the T. thermophila Ribozyme , 2003, Science.

[22]  M. Peliti,et al.  Stretching single stranded DNA, a model polyelectrolyte. , 2002, Physical review letters.

[23]  Michelle D. Wang,et al.  Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[24]  J. Richardson Rho-dependent termination and ATPases in transcript termination. , 2002, Biochimica et biophysica acta.

[25]  D. Erie The many conformational states of RNA polymerase elongation complexes and their roles in the regulation of transcription. , 2002, Biochimica et biophysica acta.

[26]  Nancy R. Forde,et al.  Using mechanical force to probe the mechanism of pausing and arrest during continuous elongation by Escherichia coli RNA polymerase , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R. Landick,et al.  The Transcriptional Regulator RfaH Stimulates RNA Chain Synthesis after Recruitment to Elongation Complexes by the Exposed Nontemplate DNA Strand , 2002, Cell.

[28]  R. Landick,et al.  Allosteric Control of RNA Polymerase by a Site That Contacts Nascent RNA Hairpins , 2001, Science.

[29]  J. Liphardt,et al.  Reversible Unfolding of Single RNA Molecules by Mechanical Force , 2001, Science.

[30]  P. Cramer,et al.  Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution , 2001, Science.

[31]  J. Roberts,et al.  Function of transcription cleavage factors GreA and GreB at a regulatory pause site. , 2000, Molecular cell.

[32]  S. Darst,et al.  A Structural Model of Transcription Elongation , 2000 .

[33]  Elena Rivas,et al.  Secondary structure alone is generally not statistically significant for the detection of noncoding RNAs , 2000, Bioinform..

[34]  R. Landick,et al.  Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[35]  M. Kashlev,et al.  The 8-Nucleotide-long RNA:DNA Hybrid Is a Primary Stability Determinant of the RNA Polymerase II Elongation Complex* , 2000, The Journal of Biological Chemistry.

[36]  R. Landick,et al.  Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[37]  K Bergman,et al.  Characterization of photodamage to Escherichia coli in optical traps. , 1999, Biophysical journal.

[38]  H. Güntherodt,et al.  Dynamic force spectroscopy of single DNA molecules. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Jeffrey W. Roberts,et al.  Mechanism of intrinsic transcription termination and antitermination. , 1999, Science.

[40]  Michelle D. Wang,et al.  Force and velocity measured for single molecules of RNA polymerase. , 1998, Science.

[41]  P. V. Hippel,et al.  An Integrated Model of the Transcription Complex in Elongation, Termination, and Editing , 1998 .

[42]  M. J. Bailey,et al.  RfaH and the ops element, components of a novel system controlling bacterial transcription elongation , 1997, Molecular microbiology.

[43]  M. Kashlev,et al.  Transcriptional arrest: Escherichia coli RNA polymerase translocates backward, leaving the 3' end of the RNA intact and extruded. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[44]  Michelle D. Wang,et al.  Stretching DNA with optical tweezers. , 1997, Biophysical journal.

[45]  D. K. Hawley,et al.  Promoter Proximal Sequences Modulate RNA Polymerase II Elongation by a Novel Mechanism , 1996, Cell.

[46]  H. Gaub,et al.  Adhesion forces between individual ligand-receptor pairs. , 1994, Science.

[47]  C. Chan,et al.  Dissection of the his leader pause site by base substitution reveals a multipartite signal that includes a pause RNA hairpin. , 1993, Journal of molecular biology.

[48]  F. A. Seiler,et al.  Numerical Recipes in C: The Art of Scientific Computing , 1989 .

[49]  C. Yanofsky,et al.  Translation activates the paused transcription complex and restores transcription of the trp operon leader region. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[50]  I. Tinoco,et al.  DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription. , 1980, Nucleic acids research.

[51]  C. Yanofsky,et al.  Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[52]  E. Nudler,et al.  Mechanistic model of the elongation complex of Escherichia coli RNA polymerase. , 1998, Cold Spring Harbor symposia on quantitative biology.

[53]  Joab R Winkler,et al.  Numerical recipes in C: The art of scientific computing, second edition , 1993 .

[54]  T Platt,et al.  Transcription termination and the regulation of gene expression. , 1986, Annual review of biochemistry.