Graphene-incorporated chitosan substrata for adhesion and differentiation of human mesenchymal stem cells.

A simple method that uses graphene to fabricate nanotopographic substrata was reported for stem cell engineering. Graphene-incorporated chitosan substrata promoted adhesion and differentiation of human mesenchymal stem cells (hMSCs). In addition, we proposed that nanotopographic cues of the substrata could enhance cell-cell and cell-material interactions for promoting functions of hMSCs.

[1]  Jiali Zhang,et al.  Biocompatibility of Graphene Oxide , 2010, Nanoscale research letters.

[2]  C. Wilkinson,et al.  The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. , 2007, Nature materials.

[3]  Qing Luo,et al.  Mesenchymal stem cells require integrin β1 for directed migration induced by osteopontin in vitro , 2011, In Vitro Cellular & Developmental Biology - Animal.

[4]  Milan Mrksich,et al.  Geometric cues for directing the differentiation of mesenchymal stem cells , 2010, Proceedings of the National Academy of Sciences.

[5]  Jian Tang,et al.  The regulation of stem cell differentiation by cell-cell contact on micropatterned material surfaces. , 2010, Biomaterials.

[6]  Aaron Tan,et al.  Nanomaterial scaffolds for stem cell proliferation and differentiation in tissue engineering. , 2013, Biotechnology advances.

[7]  R. Mahajan,et al.  Electrochemical detection of dopamine in the presence of ascorbic acid using graphene modified electrodes. , 2010, Biosensors & bioelectronics.

[8]  F. Guilak,et al.  Control of stem cell fate by physical interactions with the extracellular matrix. , 2009, Cell stem cell.

[9]  L. Matrisian,et al.  Osteopontin, a Novel Substrate for Matrix Metalloproteinase-3 (Stromelysin-1) and Matrix Metalloproteinase-7 (Matrilysin)* , 2001, The Journal of Biological Chemistry.

[10]  Christy L Haynes,et al.  Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. , 2011, ACS applied materials & interfaces.

[11]  N. Gadegaard,et al.  Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. , 2011, Nature materials.

[12]  Jianguo Tang,et al.  Strategies for chemical modification of graphene and applications of chemically modified graphene , 2012 .

[13]  Chwee Teck Lim,et al.  Origin of enhanced stem cell growth and differentiation on graphene and graphene oxide. , 2011, ACS nano.

[14]  Christopher S. Chen,et al.  Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. , 2004, Developmental cell.

[15]  Deok‐Ho Kim,et al.  Charged nanomatrices as efficient platforms for modulating cell adhesion and shape. , 2012, Tissue engineering. Part C, Methods.

[16]  Takuya Gotou,et al.  Single graphene sheet detected in a carbon nanofilm , 2004 .

[17]  Wan-Ju Li,et al.  Combinatorial screening of chemically defined human mesenchymal stem cell culture substrates. , 2012, Journal of materials chemistry.

[18]  Thomas J. Webster,et al.  Carbon nanotubes for stem cell control , 2012 .

[19]  Seeram Ramakrishna,et al.  Biomimetic composites and stem cells interaction for bone and cartilage tissue regeneration , 2012 .

[20]  Matthias P Lutolf,et al.  Artificial Stem Cell Niches , 2009, Advanced materials.

[21]  G. Pastorin,et al.  Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. , 2011, ACS nano.