Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. II. Enhancement of HPLF differentiation and aggregation on PEC by L-ascorbic acid and dexamethasone.

In addition to many types of extra cellular matrix (ECM) in vivo, cells are stimulated by many types of vitamins, hormones, growth factors, etc. In this paper the effects of L-ascorbic acid 2-phosphate (Asc-2P) and dexamethasone (Dex) on proliferation and differentiation of human periodontal ligament fibroblast (HPLF) using polyelectrolyte complex (PEC) as a matrix in vitro will be discussed. The PEC was composed of chitosan as a polycation, with carboxymethyl (CPEC) or sulfated chitin (SPEC). Asc-2P (0.2 mM) inhibited the growth of HPLF on CPEC, but promoted the growth on SPEC. Moreover, the aggregation of HPLF on CPEC was inhibited by Asc-2P, but that on SPEC was induced in the presence of Asc-2P and Dex. Although Asc-2P reduced an increase in alkaline phosphatase (ALPase) activity of HPLF on CPEC as well, it induced a twofold increase in ALPase activities on SPEC and TCD. Furthermore, in the medium containing Asc-2P and 100 mM of Dex, cell growth was inhibited, but ALPase activity was promoted on both SPEC and TCD to form many aggregates on SPEC. ALPase activity increased by twofold over that of HPLF cultured in the medium containing only Asc-2P. Therefore, it is suggested that the cell functions of HPLF are controlled by the combination of PEC and additives.

[1]  T Hamano,et al.  Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. I. Three-dimensional structure of HPLF cultured on PEC. , 1998, Journal of biomedical materials research.

[2]  B. Frenkel,et al.  Species-specific glucocorticoid and 1,25-dihydroxyvitamin D responsiveness in mouse MC3T3-E1 osteoblasts: dexamethasone inhibits osteoblast differentiation and vitamin D down-regulates osteocalcin gene expression. , 1997, Endocrinology.

[3]  G. Edelman,et al.  Glucocorticoid receptor pathways are involved in the inhibition of astrocyte proliferation. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[4]  D E Ingber,et al.  Electrically conducting polymers can noninvasively control the shape and growth of mammalian cells. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Wozney,et al.  Expression of bone morphogenetic protein messenger RNA in prolonged cultures of fetal rat calvarial cells , 1994, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[6]  J. Anderson,et al.  Protein adsorption of biomedical polymers influences activated monocytes to produce fibroblast stimulating factors. , 1992, Journal of biomedical materials research.

[7]  S. Kasugai,et al.  Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro: Inductive effects of dexamethasone on the osteoblastic phenotype , 1991, Journal of cellular physiology.

[8]  J. Aubin,et al.  Determination of the capacity for proliferation and differentiation of osteoprogenitor cells in the presence and absence of dexamethasone. , 1990, Developmental biology.

[9]  H. Senoo,et al.  L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts , 1989, Journal of cellular physiology.

[10]  M. Ohshima,et al.  Alkaline phosphatase activities of cultured human periodontal ligament cells. , 1988, The Journal of Nihon University School of Dentistry.

[11]  F. M. McLean,et al.  Roles of alkaline phosphatase and labile internal mineral in matrix vesicle-mediated calcification. Effect of selective release of membrane-bound alkaline phosphatase and treatment with isosmotic pH 6 buffer. , 1986, The Journal of biological chemistry.

[12]  B. Ratner,et al.  Hydrophilic-hydrophobic copolymers as cell substrates: Effect on 3T3 cell growth rates , 1985 .

[13]  J. Forrester,et al.  Comparison of Systolic Blood Pressure Measurements by Auscultation and Visual Manometer Needle Jump , 2019, International journal of exercise science.

[14]  K. Abe,et al.  Interactions Between Macromolecules in Solution and Intermacromolecular Complexes , 1982 .

[15]  S. Pinnell,et al.  Regulation of collagen synthesis by ascorbic acid. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[16]  E. Stanbridge,et al.  Anchorage-independent growth of normal human fibroblasts. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[17]  R. Schweiger.  Polysaccharide sulfates. I. cellulose sulfate with a high degree of substitution , 1972 .

[18]  J. Heersche,et al.  Progesterone stimulates proliferation and differentiation of osteoprogenitor cells in bone cell populations derived from adult female but not from adult male rats. , 1997, Bone.

[19]  M. Montano,et al.  Glucocorticoid effects on the skeletal muscle differentiation program: analysis of clonal proliferation, morphological differentiation and the expression of muscle-specific and regulatory genes. , 1997, Endocrine research.

[20]  Su‐Li Cheng,et al.  Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. , 1994, Endocrinology.

[21]  C. Finlay,et al.  8 – Glucocorticoid Enhancement of Cellular Proliferation in Vitro , 1987 .