JBrowse: a dynamic web platform for genome visualization and analysis

BackgroundJBrowse is a fast and full-featured genome browser built with JavaScript and HTML5. It is easily embedded into websites or apps but can also be served as a standalone web page.ResultsOverall improvements to speed and scalability are accompanied by specific enhancements that support complex interactive queries on large track sets. Analysis functions can readily be added using the plugin framework; most visual aspects of tracks can also be customized, along with clicks, mouseovers, menus, and popup boxes. JBrowse can also be used to browse local annotation files offline and to generate high-resolution figures for publication.ConclusionsJBrowse is a mature web application suitable for genome visualization and analysis.

[1]  Matthew R. Hanlon,et al.  Araport: the Arabidopsis Information Portal , 2014, Nucleic Acids Res..

[2]  Raymond K. Auerbach,et al.  A User's Guide to the Encyclopedia of DNA Elements (ENCODE) , 2011, PLoS biology.

[3]  Chris Mungall,et al.  A Chado case study: an ontology-based modular schema for representing genome-associated biological information , 2007, ISMB/ECCB.

[4]  Christian J. Stoeckert,et al.  NIA GENETICS OF ALZHEIMER'S DISEASE DATA STORAGE SITE (NIAGADS): 2014 UPDATE , 2014, Alzheimer's & Dementia.

[5]  B. S. Manjunath,et al.  The iPlant Collaborative: Cyberinfrastructure for Plant Biology , 2011, Front. Plant Sci..

[6]  Monica C Munoz-Torres,et al.  Web Apollo: a web-based genomic annotation editing platform , 2013, Genome Biology.

[7]  Ann E. Loraine,et al.  The Integrated Genome Browser: free software for distribution and exploration of genome-scale datasets , 2009, Bioinform..

[8]  Gary Moore,et al.  The i5k Workspace@NAL—enabling genomic data access, visualization and curation of arthropod genomes , 2014, Nucleic Acids Res..

[9]  L. Stein,et al.  JBrowse: a next-generation genome browser. , 2009, Genome research.

[10]  Helga Thorvaldsdóttir,et al.  Integrative Genomics Viewer , 2011, Nature Biotechnology.

[11]  Mark Yandell,et al.  MAKER2: an annotation pipeline and genome-database management tool for second-generation genome projects , 2011, BMC Bioinformatics.

[12]  Kelvin H. Lee,et al.  Chinese hamster genome database: an online resource for the CHO community at www.CHOgenome.org. , 2012, Biotechnology and bioengineering.

[13]  David M. Goodstein,et al.  Phytozome: a comparative platform for green plant genomics , 2011, Nucleic Acids Res..

[14]  S. Lewis,et al.  The generic genome browser: a building block for a model organism system database. , 2002, Genome research.

[15]  Gonçalo R. Abecasis,et al.  The Sequence Alignment/Map format and SAMtools , 2009, Bioinform..

[16]  Lukas A. Mueller,et al.  The Sol Genomics Network (SGN)—from genotype to phenotype to breeding , 2014, Nucleic Acids Res..

[17]  Ping Zheng,et al.  The Genome Database for Rosaceae (GDR): year 10 update , 2013, Nucleic Acids Res..

[18]  Weisong Liu,et al.  The Rat Genome Database 2015: genomic, phenotypic and environmental variations and disease , 2014, Nucleic Acids Res..

[19]  Jason Williams,et al.  DNA Subway: Making Genome Analysis Egalitarian , 2014, XSEDE '14.

[20]  Antony V. Cox,et al.  The Ensembl Web site: mechanics of a genome browser. , 2004, Genome research.

[21]  Gavin Williams Learn HTML5 and JavaScript for Android , 2012, Apress.

[22]  Yan Zhang,et al.  PATRIC, the bacterial bioinformatics database and analysis resource , 2013, Nucleic Acids Res..

[23]  Anton Nekrutenko,et al.  Web-based visual analysis for high-throughput genomics , 2013, BMC Genomics.

[24]  Mingming Jia,et al.  COSMIC: exploring the world's knowledge of somatic mutations in human cancer , 2014, Nucleic Acids Res..

[25]  Gavin Williams JavaScript: Models, Views, and Controllers , 2012 .

[26]  Wensheng Wang,et al.  SNP-Seek database of SNPs derived from 3000 rice genomes , 2014, Nucleic Acids Res..

[27]  Ian H Holmes,et al.  Setting Up the JBrowse Genome Browser , 2010, Current protocols in bioinformatics.

[28]  B. Korber,et al.  HIV sequence compendium 2002 , 2002 .

[29]  M. Yandell,et al.  Genome Annotation and Curation Using MAKER and MAKER‐P , 2014, Current protocols in bioinformatics.

[30]  Brian D. O'Connor,et al.  SeqWare Query Engine: storing and searching sequence data in the cloud , 2010, BMC Bioinformatics.

[31]  Tom H. Pringle,et al.  The human genome browser at UCSC. , 2002, Genome research.

[32]  Andrew Rambaut,et al.  HIV Sequence Compendium 2015 , 2015 .

[33]  Tim J. P. Hubbard,et al.  Dalliance: interactive genome viewing on the web , 2011, Bioinform..

[34]  Oscar Westesson,et al.  Visualizing next-generation sequencing data with JBrowse , 2013, Briefings Bioinform..

[35]  Gos Micklem,et al.  Supporting Online Material Materials and Methods Figs. S1 to S50 Tables S1 to S18 References Identification of Functional Elements and Regulatory Circuits by Drosophila Modencode , 2022 .

[36]  Raymond K. Auerbach,et al.  Integrative Analysis of the Caenorhabditis elegans Genome by the modENCODE Project , 2010, Science.

[37]  Kimberly Van Auken,et al.  WormBase 2014: new views of curated biology , 2013, Nucleic Acids Res..