Enhanced expression of fibronectin by cmd/cmd chondrocytes and its modulation by exogenously added proteoglycan.

Cartilage matrix deficiency (cmd/cmd) in mice is a genetic disorder associated with the failure of chondrocytes to synthesize the core protein of cartilage proteoglycan monomer (cartilage-PG). Immunohistochemical and biochemical analyses revealed enhanced accumulation of fibronectin in culture of cmd/cmd chondrocytes as well as in mutant cartilage tissue in vivo. Purified cartilage-PG, when added exogenously to a culture of cmd/cmd chondrocytes, caused a reduction in abnormal accumulation of fibronectin over several subsequent days of culturing. Using a fibronectin cDNA probe, we showed that, on the basis of total RNA, the fibronectin mRNA level was four or eight times higher in cmd/cmd chondrocytes than in the normal. The level of fibronectin mRNA in cmd/cmd cells was lowered by culturing the cells in the presence of added cartilage-PG. These findings suggest that the abnormal accumulation of fibronectin in the mutant cell culture is primarily due to elevation of fibronectin mRNA level, and that cartilage-PG in the extracellular matrix may affect the regulation of fibronectin biosynthesis at the steady-state level of mRNA.

[1]  V. Bennett,et al.  The splicing pattern of fibronectin mRNA changes during chondrogenesis resulting in an unusual form of the mRNA in cartilage. , 1991, The Journal of biological chemistry.

[2]  D. Boettiger,et al.  Modulation of fibronectin gene expression in chondrocytes by viral transformation and substrate attachment , 1987, The Journal of cell biology.

[3]  S. Suzuki,et al.  Correction of abnormal matrix formed by cmd/cmd chondrocytes in culture by exogenously added cartilage proteoglycan , 1986, The Journal of cell biology.

[4]  S. Sakiyama,et al.  Nucleotide sequence of a full-length cDNA for mouse cytoskeletal beta-actin mRNA. , 1986, Nucleic acids research.

[5]  C. West,et al.  A specificity for cellular fibronectin in its effect on cultured chondroblasts. , 1984, Differentiation; research in biological diversity.

[6]  G. Lust,et al.  Synthesis of fibronectin in normal and osteoarthritic articular cartilage. , 1984, Biochimica et biophysica acta.

[7]  H. Barrach,et al.  Presence of link protein in cartilage from cmd/cmd (cartilage matrix deficiency) mice. , 1983, Archives of biochemistry and biophysics.

[8]  E. Ruoslahti,et al.  Immunological evidence for two distinct chondroitin sulfate proteoglycan core proteins: differential expression in cartilage matrix deficient mice. , 1983, Developmental biology.

[9]  A. Kornblihtt,et al.  Isolation and characterization of cDNA clones for human and bovine fibronectins. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[10]  K M Yamada,et al.  Cell surface interactions with extracellular materials. , 1983, Annual review of biochemistry.

[11]  G. Lust,et al.  Fibronectin in osteoarthritic canine articular cartilage. , 1982, Biochemical and biophysical research communications.

[12]  R. Cancedda,et al.  The culture of chick embryo chondrocytes and the control of their differentiated functions in vitro. I. Characterization of the chondrocyte-specific phenotypes. , 1982, Experimental cell research.

[13]  J. Foidart,et al.  Immunofluorescence localization of fibronectin in chondrosarcoma cartilage matrix. , 1982, Cancer research.

[14]  H. Barrach,et al.  Absence of proteoglycan core protein in cartilage from the cmd/cmd (cartilage matrix deficiency) mouse. , 1981, The Journal of biological chemistry.

[15]  K. von der Mark,et al.  Extracellular matrix formation by chondrocytes in monolayer culture , 1981, The Journal of cell biology.

[16]  J. Foidart,et al.  Distribution of fibronectin and collagen during mouse limb and palate development. , 1981, Differentiation; research in biological diversity.

[17]  K. von der Mark,et al.  Changes in the patterns of collagens and fibronectin during limb-bud chondrogenesis. , 1980, Journal of embryology and experimental morphology.

[18]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[19]  Kenneth M. Yamada,et al.  The influence of an adhesive cell surface protein on chondrogenic expression in vitro. , 1979, Experimental cell research.

[20]  J. Rosenbloom,et al.  Fibronectin alters the phenotypic properties of cultured chick embryo chondroblasts , 1979, Cell.

[21]  K. Ito,et al.  The occurrence of low buoyant density proteoglycans in embryonic chick cartilage. , 1978, Biochemical and biophysical research communications.

[22]  J. Foidart,et al.  Antibodies to type II collagen in relapsing polychondritis. , 1978, The New England journal of medicine.

[23]  R. Timpl,et al.  Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. Response to the removal of extracellular cartilage matrix , 1978, The Journal of cell biology.

[24]  J. Hassell,et al.  Inhibition of limb chondrogenesis in vitro by vitamin A: alterations in cell surface characteristics. , 1978, Developmental biology.

[25]  L. C. Dunn,et al.  Cartilage matrix deficiency (cmd): a new autosomal recessive lethal mutation in the mouse. , 1978, Journal of embryology and experimental morphology.

[26]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .

[27]  E. Robins,et al.  The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. , 1958, The Journal of biological chemistry.