论文信息 - Rapid determination of microorganisms using a flow-injection system

Rapid determination of microorganisms using a flow-injection system

An automated method for the rapid determination of microorganisms using a flow-injection system is presented. Electrochemical measurement of a mediator reduced by microbial metabolism allowed the determination of fungi and bacteria in a few minutes. The lowest detection limit was 5 × 106 colony-forming units (cfu) ml−1 for Escherichia coli. Correlation between the flow-injection method and standard microbiological methods was excellent (r = 0.997, n = 4 for Beauveria bassiana; r = 0.997, n = 7 for E. coli). The flow-injection system was applied to the on-line control of an E. coli cultivation.

Rolf D. Schmid | R. Schmid | Thomas Ding | T. Ding

[1] S. Gibson,et al. Automated Microbiological Detection/Identification System , 1977, Journal of clinical microbiology.

[2] B Jarvis,et al. Observations on the enumeration of moulds in food and feedingstuffs. , 1983, The Journal of applied bacteriology.

[3] I. Karube,et al. Dye-coupled electrode system for the rapid determination of cell populations in polluted water , 1982, Applied and Environmental Microbiology.

[4] Y. Zhang,et al. Electrode system for determination of microbial cell populations in polluted water , 1989, Applied and environmental microbiology.

[5] D B Kell,et al. The estimation of microbial biomass. , 1985, Biosensors.

[6] G. Pettipher,et al. Rapid Membrane Filtration-Epifluorescent Microscopy Technique for Direct Enumeration of Bacteria in Raw Milk , 1980, Applied and environmental microbiology.

[7] J. Ruzicka,et al. Flow injection analyses , 1975 .

[8] H. Hill,et al. Bioelectrochemical fuel cell and sensor based on a quinoprotein, alcohol dehydrogenase , 1983 .