Blue light diminishes interaction of PAS/LOV proteins, putative blue light receptors in Arabidopsis thaliana, with their interacting partners

The light, oxygen, or voltage (LOV) domain that belongs to the Per-ARNT-Sim (PAS) domain superfamily is a blue light sensory module. The Arabidopsis thalianaPAS/LOV PROTEIN (PLP) gene encodes three putative blue light receptor proteins, PLPA, PLPB, and PLPC, because of its mRNA splicing variation. PLPA and PLPB each contain one PAS domain at the N-terminal region and one LOV domain at the C-terminal region, while the LOV domain is truncated in PLPC. RNA gel blot analysis showed that PLP mRNA was markedly expressed after exposure to salt or dehydration stress. Yeast two-hybrid screening led to the isolation of VITAMIN C DEFECTIVE 2 (VTC2), VTC2-LIKE (VTC2L), and BEL1-LIKE HOMEODOMAIN 10 proteins (BLH10A and BLH10B) as PLP-interacting proteins. The molecular interaction of PLPA with VTC2L, BLH10A or BLH10B, and that of PLPB with VTC2L were diminished when yeasts were grown under blue light illumination. Furthermore, the possible binding of flavin chromophore to PLPA and PLPB was demonstrated. These results imply that the LOV domain of PLPA and PLPB functions as a blue light sensor, and suggest the applicability of these interactions to blue light-dependent switching in transcriptional regulation in yeast or other organisms.

[1]  A. Claiborne.  Studies on the structure and mechanism of Streptococcus faecium L-alpha-glycerophosphate oxidase. , 1986, The Journal of biological chemistry.

[2]  T. Wada,et al.  RPT2: A Signal Transducer of the Phototropic Response in Arabidopsis , 2000, Plant Cell.

[3]  J. Christie,et al.  LOV (light, oxygen, or voltage) domains of the blue-light photoreceptor phototropin (nph1): binding sites for the chromophore flavin mononucleotide. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[4]  K. Yamaguchi-Shinozaki,et al.  A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis. , 1996, The Plant cell.

[5]  K. Shinozaki,et al.  Identification of ASK and clock-associated proteins as molecular partners of LKP2 (LOV kelch protein 2) in Arabidopsis. , 2004, Journal of experimental botany.

[6]  B. Bartel,et al.  FKF1, a Clock-Controlled Gene that Regulates the Transition to Flowering in Arabidopsis , 2000, Cell.

[7]  T. Todo,et al.  Photoreaction cycle of the light, oxygen, and voltage domain in FKF1 determined by low-temperature absorption spectroscopy. , 2006, Biochemistry.

[8]  Karine David,et al.  ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light. , 2007, Nature.

[9]  S. Kay,et al.  FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis , 2003, Nature.

[10]  Masahiro Kasahara,et al.  Arabidopsis nph1 and npl1: Blue light receptors that mediate both phototropism and chloroplast relocation , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[11]  D. Matsuoka,et al.  Blue light-regulated molecular switch of Ser/Thr kinase in phototropin. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[12]  D. E. Somers,et al.  ZEITLUPE Encodes a Novel Clock-Associated PAS Protein from Arabidopsis , 2000, Cell.

[13]  S. Kay,et al.  A Role for LKP2 in the Circadian Clock of Arabidopsis Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010332. , 2001, The Plant Cell Online.

[14]  P Reymond,et al.  Arabidopsis NPH1: a flavoprotein with the properties of a photoreceptor for phototropism. , 1998, Science.

[15]  Ken-ichiro Shimazaki,et al.  phot1 and phot2 mediate blue light regulation of stomatal opening , 2001, Nature.

[16]  R. Last,et al.  Identification of ascorbic acid-deficient Arabidopsis thaliana mutants. , 2000, Genetics.

[17]  J. Ecker,et al.  Phototropin-related NPL1 controls chloroplast relocation induced by blue light , 2001, Nature.

[18]  E. Schäfer,et al.  Light perception and signalling in higher plants. , 2003, Current opinion in plant biology.

[19]  J. Christie,et al.  Photochemical and mutational analysis of the FMN-binding domains of the plant blue light receptor, phototropin. , 2000, Biochemistry.

[20]  E. Liscum,et al.  Arabidopsis NPH3: A NPH1 photoreceptor-interacting protein essential for phototropism. , 1999, Science.

[21]  W. Eisenreich,et al.  An optomechanical transducer in the blue light receptor phototropin from Avena sativa , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Ishiguro,et al.  Arabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response. , 2001, Science.

[23]  W. Werr,et al.  Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana , 2006, Nucleic acids research.

[24]  R. Last,et al.  Arabidopsis Map-Based Cloning in the Post-Genome Era , 2002, Plant Physiology.

[25]  T. Kiyosue,et al.  LKP1 (LOV kelch protein 1): a factor involved in the regulation of flowering time in arabidopsis. , 2000, The Plant journal : for cell and molecular biology.

[26]  D. E. Somers,et al.  Targeted degradation of TOC1 by ZTL modulates circadian function in Arabidopsis thaliana , 2003, Nature.

[27]  K. Shinozaki,et al.  A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. , 1994, The Plant cell.

[28]  Klaus Richter,et al.  A central role of Arabidopsis thaliana ovate family proteins in networking and subcellular localization of 3-aa loop extension homeodomain proteins. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[29]  M. Van Montagu,et al.  Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[30]  P. Oeller,et al.  Arabidopsis NPH1: a protein kinase with a putative redox-sensing domain. , 1997, Science.

[31]  J. Cooney,et al.  The missing step of the l-galactose pathway of ascorbate biosynthesis in plants, an l-galactose guanyltransferase, increases leaf ascorbate content , 2007, Proceedings of the National Academy of Sciences.

[32]  C. Brenner,et al.  Arabidopsis VTC2 Encodes a GDP-l-Galactose Phosphorylase, the Last Unknown Enzyme in the Smirnoff-Wheeler Pathway to Ascorbic Acid in Plants*♦ , 2007, Journal of Biological Chemistry.

[33]  Joseph R. Ecker,et al.  CTR1, a negative regulator of the ethylene response pathway in arabidopsis, encodes a member of the Raf family of protein kinases , 1993, Cell.

[34]  Keith Moffat,et al.  The LOV domain family: photoresponsive signaling modules coupled to diverse output domains. , 2003, Biochemistry.

[35]  K. Okada,et al.  RPT2 Is a Signal Transducer Involved in Phototropic Response and Stomatal Opening by Association with Phototropin 1 in Arabidopsis thaliana , 2004, The Plant Cell Online.