The tissue culture derived sugarcane var. CoJ 64 plants attained better height, millable cane height and a greater number of live buds to conventionally raised plants. Further, the plants raised in vitro showed 13.2% increase in cane yield and 11.03% sugar recovery as compared to conventionally propagated sugarcane under parallel agronomic practices in the field, advocating suitability of seed cane programmed through tissue culture. Key words: Comparative studies, Field performance, Sugarcane, Micropropagation DOI = 10.3329/ptcb.v16i1.1102 Plant Tissue Cult. & Biotech. 16(1): 25-29, 2006 (June)