A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging

[1]  Takeharu Nagai,et al.  Five colour variants of bright luminescent protein for real-time multicolour bioimaging , 2016, Nature Communications.

[2]  M. Merkx,et al.  Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc , 2016, ACS chemical biology.

[3]  Roger Y Tsien,et al.  A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein , 2016, Nature Methods.

[4]  V. Ollendorff,et al.  Fast and high resolution single-cell BRET imaging , 2016, Scientific Reports.

[5]  K. Wood,et al.  Target engagement and drug residence time can be observed in living cells with BRET , 2015, Nature Communications.

[6]  J. Cleveland,et al.  Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis. , 2015, Cancer research.

[7]  Shinya Kuroda,et al.  An optogenetic system for interrogating the temporal dynamics of Akt , 2015, Scientific Reports.

[8]  K. Aoki,et al.  Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer , 2015, Scientific Reports.

[9]  R. Campbell,et al.  Emerging fluorescent protein technologies. , 2015, Current opinion in chemical biology.

[10]  Atsushi Miyawaki,et al.  Molecular spies for bioimaging--fluorescent protein-based probes. , 2015, Molecular cell.

[11]  Leigh A. Stoddart,et al.  Application of BRET to monitor ligand binding to GPCRs , 2015, Nature Methods.

[12]  Takeharu Nagai,et al.  Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging , 2015, Proceedings of the National Academy of Sciences.

[13]  K. Aoki,et al.  mTORC1 upregulation via ERK-dependent gene expression change confers intrinsic resistance to MEK inhibitors in oncogenic KRas-mutant cancer cells , 2015, Oncogene.

[14]  Matthew Weitzman,et al.  Optogenetic approaches to cell migration and beyond. , 2014, Current opinion in cell biology.

[15]  M. Häusser.  Optogenetics: the age of light , 2014, Nature Methods.

[16]  K. Aoki,et al.  Fluorescence resonance energy transfer based quantitative analysis of feedforward and feedback loops in epidermal growth factor receptor signaling and the sensitivity to molecular targeting drugs , 2014, The FEBS journal.

[17]  B. Cui,et al.  Light-Mediated Kinetic Control Reveals the Temporal Effect of the Raf/MEK/ERK Pathway in PC12 Cell Neurite Outgrowth , 2014, PloS one.

[18]  Jin Zhang,et al.  Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation. , 2014, Chemistry & biology.

[19]  Kazuhiro Aoki,et al.  Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation. , 2013, Molecular cell.

[20]  Zhi-jie Liu,et al.  Oxygen Activation of Apo‐obelin–Coelenterazine Complex , 2013, Chembiochem : a European journal of chemical biology.

[21]  Michael Z. Lin,et al.  Optical Control of Protein Activity by Fluorescent Protein Domains , 2012, Science.

[22]  B. Déprez,et al.  Setting Up a Bioluminescence Resonance Energy Transfer High throughput Screening Assay to Search for Protein/Protein Interaction Inhibitors in Mammalian Cells , 2012, Front. Endocrin..

[23]  Michael Z. Lin,et al.  Improving FRET dynamic range with bright green and red fluorescent proteins , 2012, Nature Methods.

[24]  Brock F. Binkowski,et al.  Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate , 2012, ACS chemical biology.

[25]  K. Aoki,et al.  Stable expression of FRET biosensors: A new light in cancer research , 2012, Cancer science.

[26]  L. Looger,et al.  Genetically encoded neural activity indicators , 2012, Current Opinion in Neurobiology.

[27]  Joachim Goedhart,et al.  Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93% , 2012, Nature Communications.

[28]  Takeharu Nagai,et al.  Luminescent proteins for high-speed single-cell and whole-body imaging , 2012, Nature Communications.

[29]  Kazuhiro Aoki,et al.  Development of an optimized backbone of FRET biosensors for kinases and GTPases , 2011, Molecular biology of the cell.

[30]  Amy E Palmer,et al.  Design and application of genetically encoded biosensors. , 2011, Trends in biotechnology.

[31]  M. Ehlers,et al.  Rapid blue light induction of protein interactions in living cells , 2010, Nature Methods.

[32]  Takahiro Kimura,et al.  Optimization of enzyme–substrate pairing for bioluminescence imaging of gene transfer using Renilla and Gaussia luciferases , 2010, The journal of gene medicine.

[33]  Patrick Bouthemy,et al.  Patch-Based Nonlocal Functional for Denoising Fluorescence Microscopy Image Sequences , 2010, IEEE Transactions on Medical Imaging.

[34]  J. Goedhart,et al.  Bright cyan fluorescent protein variants identified by fluorescence lifetime screening , 2010, Nature Methods.

[35]  Takeharu Nagai,et al.  Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators. , 2010, ACS chemical biology.

[36]  M. C. Mancini,et al.  Bioimaging: second window for in vivo imaging. , 2009, Nature nanotechnology.

[37]  Christopher A. Voigt,et al.  Spatiotemporal Control of Cell Signalling Using A Light-Switchable Protein Interaction , 2009, Nature.

[38]  B. Kuhlman,et al.  A genetically-encoded photoactivatable Rac controls the motility of living cells , 2009, Nature.

[39]  A. Bradley,et al.  Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon , 2009, Nature Methods.

[40]  Yoshihiro Nakajima,et al.  Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging , 2007, Nature Methods.

[41]  Chitra Subramanian,et al.  Imaging protein interactions with bioluminescence resonance energy transfer (BRET) in plant and mammalian cells and tissues , 2007, Proceedings of the National Academy of Sciences.

[42]  M. Fukuoka,et al.  Differential constitutive activation of the epidermal growth factor receptor in non-small cell lung cancer cells bearing EGFR gene mutation and amplification. , 2007, Cancer research.

[43]  W. Thomas,et al.  Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein-protein interactions in live cells. , 2006, Cellular signalling.

[44]  Akihiro Urasaki,et al.  Functional Dissection of the Tol2 Transposable Element Identified the Minimal cis-Sequence and a Highly Repetitive Sequence in the Subterminal Region Essential for Transposition , 2006, Genetics.

[45]  Sanjiv Sam Gambhir,et al.  Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output. , 2006, Protein engineering, design & selection : PEDS.

[46]  V. Khankaldyyan,et al.  In Vivo Testing of Renilla Luciferase Substrate Analogs in an Orthotopic Murine Model of Human Glioblastoma , 2006, Molecular imaging.

[47]  K. Eidne,et al.  Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET) , 2006, Nature Methods.

[48]  C. Contag,et al.  Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo. , 2005, Journal of biomedical optics.

[49]  Patrick S Daugherty,et al.  Evolutionary optimization of fluorescent proteins for intracellular FRET , 2005, Nature Biotechnology.

[50]  A. Miyawaki,et al.  A Pair of Fluorescent Resonance Energy Transfer-based Probes for Tyrosine Phosphorylation of the CrkII Adaptor Protein in Vivo * 210 , 2001, The Journal of Biological Chemistry.

[51]  A. Miyawaki,et al.  Spatio-temporal images of growth-factor-induced activation of Ras and Rap1 , 2001, Nature.

[52]  Yamamura Ken-ichi,et al.  Efficient selection for high-expression transfectants with a novel eukaryotic vector , 1991 .

[53]  H. Niwa,et al.  Efficient selection for high-expression transfectants with a novel eukaryotic vector. , 1991, Gene.

[54]  Whitney Scharer,et al.  The Age of Light , 2019 .

[55]  K. Sumiyama,et al.  Live imaging of protein kinase activities in transgenic mice expressing FRET biosensors. , 2012, Cell structure and function.

[56]  Kazuhiro Aoki,et al.  Visualization of small GTPase activity with fluorescence resonance energy transfer-based biosensors , 2009, Nature Protocols.