论文信息 - Monitoring cellular metabolism with fluorescence lifetime of reduced nicotinamide adenine dinucleotide

Monitoring cellular metabolism with fluorescence lifetime of reduced nicotinamide adenine dinucleotide

Fluorescence lifetime imaging has gained popularity as a sensitive technique to monitor the functional/conformational states of NADH - one of the main compounds of the oxidative phosphorylation. We hereby review the development and current application of cellular metabolism observation via NADH FLIM, illustrating it with examples of physiological and pathological states.

Fu-Jen Kao | V. Ghukasyan | F. Kao | Vladimir V. Ghukasyan

[1] Fu-Jen Kao,et al. Differentiation of apoptosis from necrosis by dynamic changes of reduced nicotinamide adenine dinucleotide fluorescence lifetime in live cells. , 2008, Journal of biomedical optics.

[2] Fu-Jen Kao,et al. Application of fluorescence resonance energy transfer resolved by fluorescence lifetime imaging microscopy for the detection of enterovirus 71 infection in cells. , 2007, Journal of biomedical optics.

[3] N. Ramanujam,et al. Metabolic mapping of MCF10A human breast cells via multiphoton fluorescence lifetime imaging of the coenzyme NADH. , 2005, Cancer research.

[4] W. Becker. Advanced Time-Correlated Single Photon Counting Techniques , 2005 .

[5] Heidrun Wabnitz,et al. High-count-rate multichannel TCSPC for optical tomography , 2001, European Conference on Biomedical Optics.

[6] D. O'connor,et al. Time-Correlated Single Photon Counting , 1984 .

[7] B. Chance,et al. Brain metabolic responses to ischemia in the mongolian gerbil: in vivo and freeze trapped redox scanning , 1983, Brain Research.

[8] H. Krebs,et al. The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. , 1967, The Biochemical journal.

[9] Otto Warburn,et al. THE METABOLISM OF TUMORS , 1931 .

[10] W. Webb,et al. Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy , 2002 .

[11] J. Lakowicz. Principles of fluorescence spectroscopy , 1983 .