Wear behavior and in vitro cytotoxicity of wear debris generated from hydroxyapatite-carbon nanotube composite coating.

This work evaluates the effect of carbon nanotube (CNT) addition to plasma-sprayed hydroxyapatite (HA) coating on its tribological behavior, biocompatibility of the coating, and cytotoxicity of CNT-containing wear debris. Biological response of the CNT-containing wear debris is critical for osteoblasts, the bone-forming cells, and macrophages, the cells that clear up wear debris from blood stream. The addition of 4 wt % CNTs to HA coating reduces the volume of wear debris generation by 80% because of the improved elastic modulus and fracture toughness. CNT reinforcement has a pronounced effect on the particle size in the wear debris and subsequent biological response. There was a slight increase in the numbers and viability of osteoblasts grown on HA-CNT compared with HA alone. The cytotoxic effect of HA and HA-CNT debris to macrophages and osteoblasts was similar, demonstrating that loose CNT does not pose a problem to these cells.

[1]  M. Matsuoka,et al.  Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes , 2010 .

[2]  S. Seal,et al.  Carbon nanotube toughened hydroxyapatite by spark plasma sintering: Microstructural evolution and multiscale tribological properties , 2010 .

[3]  S. Goodman,et al.  Cellular chemotaxis induced by wear particles from joint replacements. , 2010, Biomaterials.

[4]  Judith Klein-Seetharaman,et al.  Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation. , 2010, Nature nanotechnology.

[5]  M. Matsuoka,et al.  Thin films of single-walled carbon nanotubes promote human osteoblastic cells (Saos-2) proliferation in low serum concentrations , 2010 .

[6]  S. Seal,et al.  Synthesis of aluminum oxide coating with carbon nanotube reinforcement produced by chemical vapor deposition for improved fracture and wear resistance , 2010 .

[7]  M. Bahrololoom,et al.  Development of wear resistant NFSS-HA novel biocomposites and study of their tribological properties for orthopaedic applications. , 2010, Journal of the mechanical behavior of biomedical materials.

[8]  M. Bahrololoom,et al.  Optimizations of wear resistance and toughness of hydroxyapatite nickel free stainless steel new bio-composites for using in total joint replacement , 2010 .

[9]  L. Shaw,et al.  Nanocrystalline hydroxyapatite with simultaneous enhancements in hardness and toughness. , 2009, Biomaterials.

[10]  Woon-Ha Yoon,et al.  Mechanical and in vitro biological performances of hydroxyapatite-carbon nanotube composite coatings deposited on Ti by aerosol deposition. , 2009, Acta biomaterialia.

[11]  P. Midgley,et al.  Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells. , 2009, Biomaterials.

[12]  J. Valdés,et al.  Estradiol and lithium chloride specifically alter NMDA receptor subunit NR1 mRNA and excitotoxicity in primary cultures , 2009, Brain Research.

[13]  Hiroaki Nakamura,et al.  Multiwalled carbon nanotubes specifically inhibit osteoclast differentiation and function. , 2009, Nano letters.

[14]  C. Colby,et al.  Biomimetic hydroxyapatite coating on glass coverslips for the assay of osteoclast activity in vitro , 2009, Journal of materials science. Materials in medicine.

[15]  Khiam Aik Khor,et al.  Preparation and characterization of a novel hydroxyapatite/carbon nanotubes composite and its interaction with osteoblast-like cells , 2009 .

[16]  Min Wang,et al.  Mechanical performance of apatite/TiO2 composite coatings formed on Ti and NiTi shape memory alloy , 2008 .

[17]  W. N. Chen,et al.  Comparative proteomics profile of osteoblasts cultured on dissimilar hydroxyapatite biomaterials: An iTRAQ‐coupled 2‐D LC‐MS/MS analysis , 2008, Proteomics.

[18]  Aimin Li,et al.  Electrophoretic deposition of HA/MWNTs composite coating for biomaterial applications , 2008, Journal of materials science. Materials in medicine.

[19]  E. Schwarz,et al.  Osteoclast Precursor Interaction with Bone Matrix Induces Osteoclast Formation Directly by an Interleukin-1-mediated Autocrine Mechanism* , 2008, Journal of Biological Chemistry.

[20]  K. Balani,et al.  Wetting of carbon nanotubes by aluminum oxide , 2008, Nanotechnology.

[21]  Y. Kim,et al.  Carbon nanotubes with high bone-tissue compatibility and bone-formation acceleration effects. , 2008, Small.

[22]  Takhee Lee,et al.  A Special Issue — Selected Peer-Reviewed Papers from 2006 International Conference on Nanoscience and Nanotechnology, Gwangju, Korea , 2007 .

[23]  K. Khor,et al.  Investigation of Multiwall Carbon Nanotube Modified Hydroxyapatite on Human Osteoblast Cell Line Using iTRAQ Proteomics Technology , 2007 .

[24]  Yao Chen,et al.  Tribological behavior of plasma-sprayed carbon nanotube-reinforced hydroxyapatite coating in physiological solution. , 2007, Acta biomaterialia.

[25]  M. Kalbáčová,et al.  Influence of single-walled carbon nanotube films on metabolic activity and adherence of human osteoblasts , 2007 .

[26]  S. R. Bakshi,et al.  Role of powder treatment and carbon nanotube dispersion in the fracture toughening of plasma-sprayed aluminum oxide-carbon nanotube nanocomposite. , 2007, Journal of nanoscience and nanotechnology.

[27]  Tao Zhang,et al.  Wear studies of hydroxyapatite composite coating reinforced by carbon nanotubes , 2007 .

[28]  Arvind Agarwal,et al.  Plasma-sprayed carbon nanotube reinforced hydroxyapatite coatings and their interaction with human osteoblasts in vitro. , 2007, Biomaterials.

[29]  Ashley A. White,et al.  Hydroxyapatite–Carbon Nanotube Composites for Biomedical Applications: A Review , 2007 .

[30]  A. Iglič,et al.  Stress distribution on the hip joint articular surface during gait , 2006, Pflügers Archiv.

[31]  S. Ge,et al.  Nano-mechanical properties and biotribological behaviors of nanosized HA/partially-stabilized zirconia composites , 2005 .

[32]  T. Mizoguchi,et al.  Prostaglandin E2 Enhances Osteoclastic Differentiation of Precursor Cells through Protein Kinase A-dependent Phosphorylation of TAK1* , 2005, Journal of Biological Chemistry.

[33]  S. Bachilo,et al.  Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells. , 2004, Journal of the American Chemical Society.

[34]  K. Khor,et al.  Bone-like apatite layer formation on hydroxyapatite prepared by spark plasma sintering (SPS). , 2004, Biomaterials.

[35]  M. Hindié,et al.  Comparative particle-induced cytotoxicity toward macrophages and fibroblasts , 2003, Cell Biology and Toxicology.

[36]  Michael D. Abràmoff,et al.  Image processing with ImageJ , 2004 .

[37]  R. Miller,et al.  Long‐Range, Entangled Carbon Nanotube Networks in Polycarbonate , 2003 .

[38]  Hua Li,et al.  Effect of spark plasma sintering on the microstructure and in vitro behavior of plasma sprayed HA coatings. , 2003, Biomaterials.

[39]  B. Wei,et al.  Annealing amorphous carbon nanotubes for their application in hydrogen storage , 2003 .

[40]  F. Wei,et al.  9 % purity multi-walled carbon nanotubes by vacuum high-temperature annealing * , 2003 .

[41]  F. Wei,et al.  99.9% purity multi-walled carbon nanotubes by vacuum high-temperature annealing , 2003 .

[42]  B. Boyan,et al.  Ceramic and PMMA particles differentially affect osteoblast phenotype. , 2002, Biomaterials.

[43]  S. Tor,et al.  Spark plasma sintering of hydroxyapatite powders. , 2002, Biomaterials.

[44]  D. Howie,et al.  The effect of particle phagocytosis and metallic wear particles on osteoclast formation and bone resorption in vitro. , 2000, The Journal of arthroplasty.

[45]  N. Dahotre,et al.  Mechanical properties of laser-deposited composite boride coating using nanoindentation , 2000 .

[46]  R. Ruoff,et al.  Strength and breaking mechanism of multiwalled carbon nanotubes under tensile load , 2000, Science.

[47]  D P Pioletti,et al.  The cytotoxic effect of titanium particles phagocytosed by osteoblasts. , 1999, Journal of biomedical materials research.

[48]  T. Chambers,et al.  Prostaglandin E2 cooperates with TRANCE in osteoclast induction from hemopoietic precursors: synergistic activation of differentiation, cell spreading, and fusion. , 1999, Endocrinology.

[49]  P. Baron,et al.  Effect of fiber length on glass microfiber cytotoxicity. , 1998, Journal of toxicology and environmental health. Part A.

[50]  D. Howie,et al.  Regulation of bone cells by particle-activated mononuclear phagocytes. , 1997, The Journal of bone and joint surgery. British volume.

[51]  M. Viceconti,et al.  Design-related fretting wear in modular neck hip prosthesis. , 1996, Journal of biomedical materials research.

[52]  L. Neumann,et al.  Long-term results of Charnley total hip replacement. Review of 92 patients at 15 to 20 years. , 1994, The Journal of bone and joint surgery. British volume.

[53]  J. Galante,et al.  Bone resorption activity of particulate‐stimulated macrophages , 1993, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[54]  D. Howie,et al.  The differences in toxicity and release of bone-resorbing mediators induced by titanium and cobalt-chromium-alloy wear particles. , 1993, The Journal of bone and joint surgery. American volume.

[55]  G. Pharr,et al.  An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments , 1992 .

[56]  T. Childs,et al.  Fundamentals of friction and wear of materials , 1983 .

[57]  Brian R. Lawn,et al.  A Critical Evaluation of Indentation Techniques for Measuring Fracture Toughness: I , 1981 .

[58]  Blanchette-Mackie Ej,et al.  Transport of colloidal particles from small blood vessels correlated with cyclic changes in permeability. , 1965 .

[59]  G. Pappas,et al.  Transport of colloidal particles from small blood vessels correlated with cyclic changes in permeability. , 1965, Investigative ophthalmology.