Development of Eosinophilic Airway Inflammation and Airway Hyperresponsiveness in Mast Cell–deficient Mice

Mast cells are the main effector cells of immediate hypersensitivity and anaphylaxis. Their role in the development of allergen-induced airway hyperresponsiveness (AHR) is controversial and based on indirect evidence. To address these issues, mast cell–deficient mice (W/W  v) and their congenic littermates were sensitized to ovalbumin (OVA) by intraperitoneal injection and subsequently challenged with OVA via the airways. Comparison of OVA-specific immunoglobulin E (IgE) levels in the serum and numbers of eosinophils in bronchoalveolar lavage fluid or lung digests showed no differences between the two groups of mice. Further, measurements of airway resistance and dynamic compliance at baseline and after inhalation of methacholine were similar. These data indicate that mast cells or IgE–mast cell activation is not required for the development of eosinophilic inflammation and AHR in mice sensitized to allergen via the intraperitoneal route and challenged via the airways.

[1]  E. Gelfand,et al.  Allergen-specific IgE and IL-5 are essential for the development of airway hyperresponsiveness. , 1997, American journal of respiratory cell and molecular biology.

[2]  F. Sundler,et al.  Allergic Eosinophil-rich Inflammation Develops in Lungs and Airways of B Cell–deficient Mice , 1997, The Journal of experimental medicine.

[3]  J. Trapani,et al.  Granzyme B (GraB) Autonomously Crosses the Cell Membrane and Perforin Initiates Apoptosis and GraB Nuclear Localization , 1997, The Journal of experimental medicine.

[4]  E. Gelfand,et al.  Antiinterleukin-5 antibody prevents airway hyperresponsiveness in a murine model of airway sensitization. , 1997, American journal of respiratory and critical care medicine.

[5]  P. Marrack,et al.  Allergic airway sensitization induces T cell activation but not airway hyperresponsiveness in B cell-deficient mice. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[6]  E. Gelfand,et al.  Passive transfer of immediate hypersensitivity and airway hyperresponsiveness by allergen-specific immunoglobulin (Ig) E and IgG1 in mice. , 1996, The Journal of clinical investigation.

[7]  J. Drazen,et al.  Sorting out the cytokines of asthma , 1996, The Journal of experimental medicine.

[8]  S. Galli,et al.  Mast‐cell—leukocyte cytokine cascades in allergic inflammation , 1995, Allergy.

[9]  R. Egan,et al.  Mast cells modulate allergic pulmonary eosinophilia in mice. , 1995, American journal of respiratory cell and molecular biology.

[10]  B. Stripp,et al.  Airway epithelial cell expression of interleukin-6 in transgenic mice. Uncoupling of airway inflammation and bronchial hyperreactivity. , 1994, The Journal of clinical investigation.

[11]  D. Friend,et al.  Lack of expression of the tryptase mouse mast cell protease 7 in mast cells of the C57BL/6J mouse. , 1994, Journal of immunology.

[12]  R. Pauwels,et al.  Attenuation of allergic airway inflammation in IL‐4 deficient mice , 1994, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

[13]  B. Stripp,et al.  Uncoupling of Airway Inflammation and Bronchial Hyperreactivity , 1994 .

[14]  P. Smith,et al.  Combined Staining Method for the Demonstration of Tissue Eosinophils and Mast Cells , 1993 .

[15]  W. Mitzner,et al.  Cationic proteins alter smooth muscle function by an epithelium-dependent mechanism. , 1993, Journal of applied physiology.

[16]  J. Drazen,et al.  Mast cell activation enhances airway responsiveness to methacholine in the mouse. , 1993, Journal of Clinical Investigation.

[17]  S. Galli,et al.  New concepts about the mast cell. , 1993, The New England journal of medicine.

[18]  C. Irvin,et al.  Cationic proteins induce airway hyperresponsiveness dependent on charge interactions. , 1993, The American review of respiratory disease.

[19]  E. Gelfand,et al.  Airway response to electrical field stimulation in sensitized inbred mice. Passive transfer of increased responsiveness with peribronchial lymph nodes. , 1992, The Journal of clinical investigation.

[20]  M. Suko,et al.  Experimental pulmonary eosinophilia in mice by Ascaris suum extract. , 1990, The American review of respiratory disease.

[21]  J. Kinet.  The high-affinity receptor for IgE. , 1990, Current opinion in immunology.

[22]  W. Paul,et al.  Mast cell lines produce lymphokines in response to cross-linkage of FcεRI or to calcium ionophores , 1989, Nature.

[23]  J. Drazen,et al.  Role of mast cells in anaphylaxis. Evidence for the importance of mast cells in the cardiopulmonary alterations and death induced by anti-IgE in mice. , 1989, The Journal of clinical investigation.

[24]  D. Chadwick.  IgE, mast cells, and the allergic response , 1989 .

[25]  W. Paul,et al.  Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores. , 1989, Nature.

[26]  S. Galli.  Mast cells: a new approach for analyzing their maturation and function in vivo. , 1988, Allergy proceedings : the official journal of regional and state allergy societies.

[27]  J. Drazen,et al.  Pulmonary responses to bronchoconstrictor agonists in the mouse. , 1988, Journal of applied physiology.

[28]  G. Alcaraz,et al.  The receptor with high affinity for immunoglobulin E. , 1986, Annual review of immunology.

[29]  K. Austen,et al.  Antigen-initiated release of platelet-activating factor (PAF-acether) from mouse bone marrow-derived mast cells sensitized with monoclonal IgE. , 1983, Journal of immunology.

[30]  S. Wasserman.  Mediators of immediate hypersensitivity. , 1983, The Journal of allergy and clinical immunology.

[31]  E. Middleton,et al.  Allergy, principles and practice , 1978 .

[32]  L. Jaques,et al.  THE MAST CELL/HEPARIN PARADOX , 1977, The Lancet.

[33]  K. Austen,et al.  Pharmacologic inhibition of the antigen-induced release of histamine and slow reacting substance of anaphylaxis (SRS-A) from monkey lung tissues mediated by human IgE. , 1971, Journal of immunology.

[34]  C. Linsell,et al.  The alpha 1-fetoprotein test in hepatocellular carcinoma. , 1970, Lancet.

[35]  Michael Schwenk,et al.  Anaphylaxis , 1919, The Indian medical gazette.