The effect of collagen-binding NGF-beta on the promotion of sciatic nerve regeneration in a rat sciatic nerve crush injury model.

Nerve growth factor plays a critical role in peripheral nerve regeneration. However, the lack of efficient NGF delivery approach limits its clinical application. It has demonstrated in our previous work that the native human NGF-beta (NAT-NGF) fused with a collagen-binding domain (CBD) could bind to collagen specifically. Since collagen is the major component of nerve extracellular matrix, we speculated that the collagen-binding NGF would target to nerve cells and improve their regeneration. In this report, we found that the fusion protein could specifically bind to endogenous collagen of the rat sciatic nerves and maintain NGF activity both in vitro and in vivo. In the rat sciatic nerve crush injury model, we found that collagen-binding NGF could be retained and concentrated at the nerve injured site to promote nerve repair and enhance function recovery following nerve damage. Thus, the collagen-binding NGF could improve the repair of peripheral nerve injury.

[1]  W. Campbell,et al.  Evaluation and management of peripheral nerve injury , 2008, Clinical Neurophysiology.

[2]  S. Mackinnon,et al.  Functional Evaluation of Complete Sciatic, Peroneal, and Posterior Tibial Nerve Lesions in the Rat , 1989, Plastic and reconstructive surgery.

[3]  D. Cornblath,et al.  Wallerian degeneration in human nerves: Serial electrophysiological studies , 1992, Muscle & nerve.

[4]  Guido Stoll,et al.  Nerve Injury, Axonal Degeneration and Neural Regeneration: Basic Insights , 1999, Brain pathology.

[5]  S. Smirnov,et al.  Analysis of basement membrane self-assembly and cellular interactions with native and recombinant glycoproteins. , 2002, Methods in Cell Biology.

[6]  M. Siqueira,et al.  Correlation between parameters of electrophysiological, histomorphometric and sciatic functional index evaluations after rat sciatic nerve repair. , 2006, Arquivos de neuro-psiquiatria.

[7]  D. Olton,et al.  Human nerve growth factor improves spatial memory in aged but not in young rats , 1994, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[8]  W. Litchy,et al.  Recombinant human nerve growth factor in the treatment of diabetic polyneuropathy , 1998, Neurology.

[9]  S. Mackinnon,et al.  Functional recovery and histomorphometric assessment following tibial nerve injury in the mouse. , 2003, Journal of reconstructive microsurgery.

[10]  L. Maffei,et al.  Intranasal administration of nerve growth factor (NGF) rescues recognition memory deficits in AD11 anti-NGF transgenic mice. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[11]  C. M. Agrawal,et al.  Technique to control pH in vicinity of biodegrading PLA-PGA implants. , 1997, Journal of biomedical materials research.

[12]  A L Dellon,et al.  Selection of the Appropriate Parameter to Measure Neural Regeneration , 1989, Annals of plastic surgery.

[13]  I. Shin,et al.  J O U R N a L O F Veterinary Science , 2010 .

[14]  E. Engvall,et al.  Neurite-promoting factors and extracellular matrix components accumulating in vivo within nerve regeneration chambers , 1984, Brain Research.

[15]  F. Gage,et al.  Gene therapy in the adult primate brain: intraparenchymal grafts of cells genetically modified to produce nerve growth factor prevent cholinergic neuronal degeneration. , 1996, Gene therapy.

[16]  Daniel H. Kim,et al.  Management and results of sciatic nerve injuries: a 24-year experience. , 1998, Journal of neurosurgery.

[17]  J. Yates,et al.  The effect of brain-derived neurotrophic factor on sensory and autonomic function after lingual nerve repair , 2004, Experimental Neurology.

[18]  M. Brenner,et al.  Controlled release of nerve growth factor enhances sciatic nerve regeneration , 2003, Experimental Neurology.

[19]  Xin-Fu Zhou,et al.  Endogenous BDNF is required for myelination and regeneration of injured sciatic nerve in rodents , 2000, The European journal of neuroscience.

[20]  H. M. Liu.  The role of extracellular matrix in peripheral nerve regeneration: a wound chamber study , 2004, Acta Neuropathologica.

[21]  A. Björklund,et al.  Protection of the Neostriatum against Excitotoxic Damage by Neurotrophin-Producing, Genetically Modified Neural Stem Cells , 1996, The Journal of Neuroscience.

[22]  Hanry Yu,et al.  Peripheral nerve regeneration with sustained release of poly(phosphoester) microencapsulated nerve growth factor within nerve guide conduits. , 2003, Biomaterials.

[23]  A. Koeppen,et al.  Wallerian degeneration: history and clinical significance , 2004, Journal of the Neurological Sciences.

[24]  James P. Hollowell,et al.  Nerve growth factor enhances regeneration through silicone chambers , 1989, Experimental Neurology.

[25]  X. Navarro,et al.  Neural plasticity after peripheral nerve injury and regeneration , 2007, Progress in Neurobiology.

[26]  Steven G Potkin,et al.  A phase 1 clinical trial of nerve growth factor gene therapy for Alzheimer disease , 2005, Nature Medicine.

[27]  K. Unsicker,et al.  Pharmacological effects of nerve growth factor and fibroblast growth factor applied to the transectioned sciatic nerve on neuron death in adult rat dorsal root ganglia , 1987, Neuroscience Letters.

[28]  B. Winblad,et al.  Intracerebroventricular Infusion of Nerve Growth Factor in Three Patients with Alzheimer’s Disease , 1998, Dementia and Geriatric Cognitive Disorders.

[29]  J. Riggs.  Recombinant human nerve growth factor in the treatment of diabetic polyneuropathy. , 1998, Neurology.

[30]  Bing Chen,et al.  Promotion of peripheral nerve growth by collagen scaffolds loaded with collagen-targeting human nerve growth factor-beta. , 2007, Journal of biomedical materials research. Part A.

[31]  J. Benoit,et al.  Intraseptal implantation of NGF-releasing microspheres promote the survival of axotomized cholinergic neurons. , 2000, Biomaterials.

[32]  D. B. Myers,et al.  Proximal and distal changes in collagen content of peripheral nerve that follow transection and crush lesions , 1986, Experimental Neurology.

[33]  Jui-Sheng Sun,et al.  Biocompatibility of NGF-grafted GTG membranes for peripheral nerve repair using cultured Schwann cells. , 2004, Biomaterials.

[34]  P. Soucacos,et al.  Sciatic and peroneal nerve injuries , 2006, Microsurgery.

[35]  L. Dahlin,et al.  Prospective study of patients with injuries to the hand and forearm: Costs, function, and general health , 2005, Scandinavian journal of plastic and reconstructive surgery and hand surgery.

[36]  T. Gordon,et al.  The cellular and molecular basis of peripheral nerve regeneration , 1997, Molecular Neurobiology.