Viability of dielectrophoretically trapped neural cortical cells in culture

Negative dielectrophoretic trapping of neural cells is an efficient way to position neural cells on the electrode sites of planar micro-electrode arrays. The preservation of viability of the neural cells is essential for this approach. This study investigates the viability of postnatal cortical rat cells that were dielectrophoretically trapped. Morphological characteristics as well as the ratio of the number of outgrowing to the number of non-outgrowing cortical cells were used to compare the viability of trapped cells to that of non-exposed cells. The morphological characteristics include the area of the cell, representing adhesive properties, and the number and length of the processes, as a measure for functional recovery. The results presented in this paper show that the viable state of dielectrophoretically trapped postnatal cortical rat cells under the conditions used was similar to that of non-exposed cells.

[1]  H. A. Pohl,et al.  Some Effects of Nonuniform Fields on Dielectrics , 1958 .

[2]  G. Banker,et al.  Culturing nerve cells , 1998 .

[3]  Enrico Marani,et al.  Dielectrophoretic trapping of dissociated fetal cortical rat neurons , 2001, IEEE Transactions on Biomedical Engineering.

[4]  Hywel Morgan,et al.  Dielectrophoretic separation of nano-particles , 1997 .

[5]  J. Pine.  Recording action potentials from cultured neurons with extracellular microcircuit electrodes , 1980, Journal of Neuroscience Methods.

[6]  W. Regehr,et al.  A long-term in vitro silicon-based microelectrode-neuron connection , 1988, IEEE Transactions on Biomedical Engineering.

[7]  H Morgan,et al.  Separation of submicron bioparticles by dielectrophoresis. , 1999, Biophysical journal.

[8]  Ulrich Zimmermann,et al.  Electromanipulation of cells , 1996 .

[9]  P. S. Wolters,et al.  Towards an improved serum-free, chemically defined medium for long-term culturing of cerebral cortex tissue , 1984, Neuroscience & Biobehavioral Reviews.

[10]  S G Shirley,et al.  Cell handling and characterization using micron and submicron electrode arrays: state of the art and perspectives of semiconductor microtools , 1995 .

[11]  Y. Tai,et al.  The neurochip: a new multielectrode device for stimulating and recording from cultured neurons , 1999, Journal of Neuroscience Methods.

[12]  A. Irimajiri,et al.  Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. I. Dielectric behavior of cell suspensions in 0.01-500 MHz and its simulation with a single-shell model. , 1987, Biochimica et biophysica acta.

[13]  G. Fuhr,et al.  Cultivation of cells under strong ac-electric field—differentiation between heating and trans-membrane potential effects , 1998 .

[14]  K. Kaler,et al.  Dielectrophoretic spectra of single cells determined by feedback-controlled levitation. , 1990, Biophysical journal.

[15]  G. Fuhr,et al.  Three-dimensional electric field traps for manipulation of cells--calculation and experimental verification. , 1993, Biochimica et biophysica acta.

[16]  David W. Tank,et al.  Sealing cultured invertebrate neurons to embedded dish electrodes facilitates long-term stimulation and recording , 1989, Journal of Neuroscience Methods.

[17]  A. Shahar.  A Dissection and tissue culture manual of the nervous system , 1989 .

[18]  H. A. Pohl.  Some effects of non-uniform fields on dielectrics , 1957 .

[19]  A New Method for the Investigation of Cellular Dielectrophoresis , 1989, Zeitschrift fur Naturforschung. C, Journal of biosciences.

[20]  F. Hefti,et al.  Growth of dissociated neurons in culture dishes coated with synthetic polymeric amines , 1984, Neuroscience Letters.

[21]  Markus Meister,et al.  Multi-neuronal signals from the retina: acquisition and analysis , 1994, Journal of Neuroscience Methods.

[22]  T. Tsong,et al.  Dielectrophoresis and electrorotation of neurospora slime and murine myeloma cells. , 1991, Biophysical journal.

[23]  G. Rebel,et al.  Neuronal cells mature faster on polyethyleneimine coated plates than on polylysine coated plates , 1992, Journal of neuroscience research.

[24]  G. Fuhr,et al.  Cell manipulation and cultivation under a.c. electric field influence in highly conductive culture media. , 1994, Biochimica et biophysica acta.