Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens.

In the present study, biosynthesis of silver nanoparticles and its activity on water borne bacterial pathogens were investigated. Silver nanoparticles were rapidly synthesized using leaf extract of Acalypha indica and the formation of nanoparticles was observed within 30min. The results recorded from UV-vis spectrum, scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS) support the biosynthesis and characterization of silver nanoparticles. From high-resolution transmission electron microscopy (HRTEM) analysis, the size of the silver nanoparticles was measured 20-30nm. Further, the antibacterial activity of synthesized silver nanoparticles showed effective inhibitory activity against water borne pathogens Viz., Escherichia coli and Vibrio cholerae. Silver nanoparticles 10microg/ml were recorded as the minimal inhibitory concentration (MIC) against E. coli and V. cholerae. Alteration in membrane permeability and respiration of the silver nanoparticle treated bacterial cells were evident from the activity of silver nanoparticles.

[1]  A. Bard,et al.  Interaction of silver(I) ions with the respiratory chain of Escherichia coli: an electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+. , 2005, Biochemistry.

[2]  A. Marshall,et al.  The mechanism of metal nanoparticle formation in plants: limits on accumulation , 2009 .

[3]  Absar Ahmad,et al.  Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. , 2004, Journal of colloid and interface science.

[4]  Li Zhang,et al.  Green synthesis of silver nanoparticles using Capsicum annuum L. extract , 2007 .

[5]  K. Janardhanan,et al.  Antibacterial Activity of Three Macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus Occurring in South India , 2003 .

[6]  Beom Soo Kim,et al.  Rapid biological synthesis of silver nanoparticles using plant leaf extracts , 2009, Bioprocess and biosystems engineering.

[7]  A. Nahrstedt,et al.  Flavonoids from Acalypha indica. , 2006, Fitoterapia.

[8]  M. Kowshik,et al.  Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3 , 2002 .

[9]  Keita Hara,et al.  Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis , 2005, Applied and Environmental Microbiology.

[10]  M. Yacamán,et al.  The bactericidal effect of silver nanoparticles , 2005, Nanotechnology.

[11]  Ahmad Reza Shahverdi,et al.  Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: A novel biological approach , 2007 .

[12]  Absar Ahmad,et al.  BIOSYNTHESIS OF METAL NANOPARTICLES USING FUNGI AND ACTINOMYCETE , 2003 .

[13]  Absar Ahmad,et al.  Geranium Leaf Assisted Biosynthesis of Silver Nanoparticles , 2003, Biotechnology progress.

[14]  A. D. Russell,et al.  Antiseptics and Disinfectants: Activity, Action, and Resistance , 2001, Clinical Microbiology Reviews.

[15]  E. M. Egorova,et al.  Synthesis of metallic nanoparticles in reverse micelles in the presence of quercetin , 2000 .

[16]  Sudheer Kumar Singh,et al.  Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain , 2009 .

[17]  A. Eslamifar,et al.  Intra/extracellular biosynthesis of silver nanoparticles by an autochthonous strain of Proteus mirabilis isolated from photographic waste. , 2009, Journal of biomedical nanotechnology.

[18]  Jiale Huang,et al.  Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf , 2007 .

[19]  Paul Mulvaney,et al.  Surface Plasmon Spectroscopy of Nanosized Metal Particles , 1996 .

[20]  N. Rajendiran,et al.  Phyllanthin-assisted biosynthesis of silver and gold nanoparticles: a novel biological approach , 2009 .

[21]  Absar Ahmad,et al.  Synthesis of Gold Nanotriangles and Silver Nanoparticles Using Aloevera Plant Extract , 2006, Biotechnology progress.

[22]  P. Bragg,et al.  The effect of silver ions on the respiratory chain of Escherichia coli. , 1974, Canadian journal of microbiology.

[23]  Nelson Durán,et al.  Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains , 2005, Journal of nanobiotechnology.

[24]  A. Ingle,et al.  Synthesis of Silver Nanoparticles Using Callus Extract of Carica papaya — A First Report , 2012, Journal of Plant Biochemistry and Biotechnology.

[25]  M. Mahmoud,et al.  Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. , 2007, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[26]  Dianne K. Newman,et al.  A role for excreted quinones in extracellular electron transfer , 2000, Nature.

[27]  Jorge L Gardea-Torresdey,et al.  Synthesis of plant-mediated gold nanoparticles and catalytic role of biomatrix-embedded nanomaterials. , 2007, Environmental science & technology.

[28]  O. Lev,et al.  The interaction of silver ions and hydrogen peroxide in the inactivation of E. coli: A preliminary evaluation of a new long acting residual drinking water disinfectant , 1995 .

[29]  K. Klabunde,et al.  Metal Oxide Nanoparticles as Bactericidal Agents , 2002 .

[30]  I. Sondi,et al.  Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. , 2004, Journal of colloid and interface science.

[31]  Shuhong Yu,et al.  Formation of silver nanowires by a novel solid-liquid phase arc discharge method , 1999 .