Hydrocarbon pasting liquids for improved tyrosinase‐based carbon‐paste phenol biosensors

Short-chain hydrocarbons are used as pasting liquids in carbon-paste tyrosinase amperometric biosensors. The response of the phenolic substrates decreases rapidly upon increasing the chain length of the hydrocarbon binder from C10 to C14, and then it levels off to a size similar to that of the mineral oil biosensor. For example, the dodecane-based enzyme electrode offers a 17.8-fold signal enhancement compared to the mineral-oil one. Such sensitivity enhancements are attributed to the extractive accumulation of the phenolic substrates. The change in the carbon-paste binder influences also the selectivity of the tyrosinase electrode and the Km, app values. Flow-injection analysis yields a detection limit of 6 nM catechol and a relative standard deviation of 2.5% (n = 30). A dual enzyme electrode chromatographic detection, based on the use of different pasting liquids, provides unique characterization of the phenolic substrates. The merits of this strategy are illustrated in connection with a river water sample.

[1]  Jenny Emnéus,et al.  Flow injection analysis of phenolic compounds with carbon paste electrodes modified with tyrosinase purchased from different companies , 1996 .

[2]  Y. Michotte,et al.  Study of a new solid carbon paste tyrosinase-modified amperometric biosensor for the determination of catecholamines by high-performance liquid chromatography , 1996 .

[3]  L. Gorton,et al.  Development of enzyme-based amperometric sensors for the determination of phenolic compounds , 1995 .

[4]  H. Lidén,et al.  Effects of different additives on a tyrosinase based carbon paste electrode , 1995 .

[5]  Shaojun Dong,et al.  CONSTRUCTION OF A TYROSINASE-BASED BIOSENSOR IN PURE ORGANIC-PHASE , 1995 .

[6]  Klaus-Dieter Vorlop,et al.  Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols , 1995 .

[7]  Lo Gorton,et al.  Carbon paste electrodes modified with enzymes, tissues, and cells , 1995 .

[8]  L. Gorton,et al.  Phenol oxidase-based biosensors as selective detection units in column liquid chromatography for the determination of phenolic compounds , 1994 .

[9]  Y. Issa,et al.  New Ampicillin Selective Plastic Membrane and Coated Metal Electrodes Based on Ampicillinium Phosphotungstate Ion pair. , 1994 .

[10]  J. Wang,et al.  Amperometric biosensor for phenols based on a tyrosinase-graphite-epoxy biocomposite. , 1994, The Analyst.

[11]  Joseph Wang,et al.  Organic-phase biosensors based on the entrapment of enzymes within poly(ester-sulfonic acid) coatings , 1993 .

[12]  E. Domínguez,et al.  Liquid chromatographic separation of phenolic drugs using catalytic detection: comparison of an enzyme reactor and enzyme electrode. , 1992, Journal of pharmaceutical and biomedical analysis.

[13]  W. Flurkey,et al.  A biochemistry project to study mushroom tyrosinase: Enzyme localization, isoenzymes, and detergent activation , 1992 .

[14]  M. Smyth,et al.  Tissue- and microbe-based electrochemical detectors for liquid chromatography , 1990 .

[15]  Joseph Wang,et al.  Solvent extraction studies with carbon paste electrodes , 1985 .

[16]  Joseph Wang,et al.  Extractive preconcentration of organic compounds at carbon paste electrodes , 1984 .

[17]  P. Kissinger,et al.  Isolation and identification of benzene metabolites in vitro with liquid chromatography/electrochemistry , 1982 .

[18]  T. Kuwana,et al.  Electrooxidation or Reduction of Organic Compounds into Aqueous Solutions Using Carbon Paste Electrode. , 1964 .