A new DNA extraction method for automated food analysis

Effective DNA purification is essential for reliable composition and contamination analysis in the food industry. This paper compares two DNA extraction and purification procedures for food analysis. The commercially available DNA extraction kit from Agilent Technologies, Inc. was tested against a new solid phase extraction (SPE) method using silica beads embedded in a porous monolith structure that is integrated in a disposable column design. The technique is amenable to automation and streamlined workflow integration, with possible miniaturization of the sample handling process making it suitable for high-throughput applications. To demonstrate the automated process, the manual homogenizing step was compared to a custom automated procedure. To demonstrate the process for food testing, two types of sausages were analyzed for traces of porcine gDNA.

[1]  S. Bhaduri,et al.  A comparison of sample preparation methods for PCR detection of pathogenic Yersinia enterocolitica from ground pork using swabbing and slurry homogenate techniques. , 2003, Molecular and cellular probes.

[2]  C. Klapperich,et al.  Thermoplastic microfluidic device for on-chip purification of nucleic acids for disposable diagnostics. , 2006, Analytical chemistry.

[3]  Carmela Martino,et al.  A comparison of DNA extraction methods for food analysis , 2007 .

[4]  L. Schwartzbrod,et al.  Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification. , 1999, Journal of virological methods.

[5]  Jean M. J. Fréchet,et al.  Surface Functionalization of Thermoplastic Polymers for the Fabrication of Microfluidic Devices by Photoinitiated Grafting , 2003 .

[6]  J. Lüthy,et al.  Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. , 1995, Journal of AOAC International.

[7]  Catherine M. Klapperich,et al.  Microfluidics-based extraction of viral RNA from infected mammalian cells for disposable molecular diagnostics , 2008 .

[8]  Frantisek Svec,et al.  Monolithic Stationary Phases for Capillary Electrochromatography Based on Synthetic Polymers: Designs and Applications , 2000 .

[9]  Andre Sharon,et al.  An automated low cost instrument for simultaneous multi-sample tissue homogenization , 2011 .

[10]  Juan G. Santiago,et al.  High-pressure electroosmotic pumps based on porous polymer monoliths , 2004 .

[11]  Rolf Meyer,et al.  Development and application of DNA analytical methods for the detection of GMOs in food , 1999 .

[12]  Markus Lipp,et al.  Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs , 2001 .

[13]  T J David,et al.  Adverse reactions and intolerance to foods. , 2000, British medical bulletin.

[14]  Scott H Sicherer,et al.  Prevalence of peanut and tree nut allergy in the United States determined by means of a random digit dial telephone survey: a 5-year follow-up study. , 2003, The Journal of allergy and clinical immunology.

[15]  T. Rohr,et al.  Porous polymer monoliths: Simple and efficient mixers prepared by direct polymerization in the channels of microfluidic chips , 2001, Electrophoresis.

[16]  Belinda Bateman,et al.  Rising prevalence of allergy to peanut in children: Data from 2 sequential cohorts. , 2002, The Journal of allergy and clinical immunology.

[17]  M. Salimans,et al.  Rapid and simple method for purification of nucleic acids , 1990, Journal of clinical microbiology.

[18]  C. Klapperich,et al.  Mechanical and chemical analysis of plasma and ultraviolet-ozone surface treatments for thermal bonding of polymeric microfluidic devices. , 2007, Lab on a chip.

[19]  M. Estes,et al.  Detection of enteric viruses in oysters by using the polymerase chain reaction , 1993, Applied and environmental microbiology.

[20]  Andre Sharon,et al.  RNA isolation from mammalian cells using porous polymer monoliths: an approach for high-throughput automation. , 2010, Analytical chemistry.