IDENTIFICATION OF TRUE HYBRID PROGENIES IN CASSAVA USING SIMPLE SEQUENCE REPEAT (SSR) MARKERS

For the maping of Cassava mosaic disease (CMD) resistance gene, CO2 and MNga-1 parents were used for hybridization to develop F 1 hybrid seeds and 153 progenies were planted in the field for genotyping. Out of the 75 SSR primers used for parental polymorphism, 57 produced polymorphic bands between parents and these were used to screen the 153 progenies of the cross for genotyping. Out of these 57 SSR primer pairs SSRY32, SSRY36, SSRY83, SSRY339, NS149 and NS890 loci produced single marker which clearly distinguished the parents. Based on the said six primers, 12 progenies out of 153 were identified as self pollinated seeds of CO2 female parent. So these types of SSR markers were very much useful for identification of true hybrid seedlings in the early stage of growth and genotyping of the progenies more accurately for gene mapping. DOI: http://dx.doi.org/10.3329/bjb.v42i1.15906 Bangladesh J. Bot. 42 (1): 155-159, 2013 (June)

[1]  V. Parkhi,et al.  Identification of Cotton Hybrid through the Combination of PCR Based RAPD, ISSR and Microsatellite Markers , 2012, Journal of Plant Biochemistry and Biotechnology.

[2]  M. Raut,et al.  Identification and genetic purity testing of cotton F 1 hybrid using molecular markers , 2011 .

[3]  M. Amjad,et al.  Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers. , 2011, Genetics and molecular research : GMR.

[4]  Juan A. Fernández,et al.  Identification of F1 hybrids of artichoke by ISSR markers and morphological analysis , 2011, Molecular Breeding.

[5]  L. Tian,et al.  Rapid and Reliable Purity Identification of F1 Hybrids of Maize (Zea may L.) Using SSR Markers , 2010 .

[6]  Y. Zafar,et al.  PARENTAGE CONFIRMATION OF COTTON HYBRIDS USING MOLECULAR MARKERS , 2009 .

[7]  Q. H. Yang,et al.  Identification of intergeneric hybrids between Saccharum spp. and Erianthus fulvus with ITSs , 2009 .

[8]  S. Te-chato,et al.  Application of molecular markers in the hybrid verification and assessment of somaclonal variation from oil palm propagated in vitro , 2009 .

[9]  A. Arzani,et al.  Identification of rice hybrids using microsatellite and RAPD markers , 2009 .

[10]  M. Guo-bin,et al.  SSR markers for identification of purity of melon hybrids , 2008 .

[11]  G. Burow,et al.  Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis , 2008 .

[12]  O. Odunola,et al.  SSR markers reveal genetic variation between improved cassava cultivars and landraces within a collection of Nigerian cassava germplasm , 2007 .

[13]  Liwang Liu,et al.  Evaluation of Genetic Purity of F1 Hybrid Seeds in Cabbage with RAPD, ISSR, SRAP, and SSR Markers , 2007 .

[14]  James B. Hicks,et al.  A plant DNA minipreparation: Version II , 1983, Plant Molecular Biology Reporter.

[15]  Xin Yeyun,et al.  Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers , 2005 .

[16]  R. Sharma,et al.  Molecular fingerprinting of hybrids and assessment of genetic purity of hybrid seeds in rice using microsatellite markers , 2004, Euphytica.

[17]  O. Panaud,et al.  Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.) , 1996, Molecular and General Genetics MGG.

[18]  H. Xi-lin,et al.  Application of molecular marker in variety identification and purity testing in vegetable crops , 2004 .

[19]  N. Manigbas,et al.  Microsatellite markers in hybridity tests to identify true hybrids of sugarcane. , 2004 .

[20]  R. Sonti,et al.  Assessment of purity of rice hybrids using microsatellite and STS markers , 2002 .

[21]  U. Gullberg,et al.  Simple sequence repeat (SSR) markers survey of the cassava (Manihot esculenta Crantz) genome: towards an SSR-based molecular genetic map of cassava , 2001, Theoretical and Applied Genetics.

[22]  Wu Yao.  Detecting Polymorphism among Upland Cotton (Gossypium hirsutum L.)Cultivars and Their Roles in Seed Purity of Hybrids with SSR Markers , 2001 .

[23]  R. Hillocks,et al.  Cassava botany and physiology. , 2001 .

[24]  P. Bhalla,et al.  RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleracea var. capitata) cultivar. , 2000, Genome.