Cross-complexing pattern of plant histones.

Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b differ markedly from their mammalian counterparts. Pea H2b has a molecular weight approximately 20% greater than that of calf thymus H2b; the amino acid compositions of the two proteins are different. Calf thymus H2a exists as a single molecular weight species, while pea H2a exists as two species which differ by about 1500 daltons. The larger plant H2a is about 19% greater in molecular weight than calf thymus H2a. The smaller is about 8% greater. Despite these differences between calf and pea histones, the strong interactions between histone pairs H3 and H4, H2b and H4, and H2a and H2b, previously demonstrated for calf histones, also exist for pea histones. There are also weak interactions between pea H2a and H4 and between pea H2b and H3, and an interaction of intermediate strength between H2a and H3. The cross-complexing pattern of the plant histones is therefore the same as that reported for calf thymus histones [D'Anna, J. A., Jr., and Isenberg, I. (1974), Biochemistry 13, 4992], despite the dissimilarities of H2a and H2b.

[1]  S. Spiker.  Identification of histones F2b and F1 in stained gels. , 1976, Journal of chromatography.

[2]  B. Wakim,et al.  Identification and fractionation of plant histones. , 1976, Archives of biochemistry and biophysics.

[3]  H. Martinson,et al.  Histone-histone interactions within chromatin. Preliminary characterization of presumptive H2B-H2A and H2B-H4 binding. , 1976, Biochemistry.

[4]  J. O. Thomas,et al.  Yeast chromatin structure , 1976, FEBS letters.

[5]  G. Felsenfeld,et al.  The organization of histones and DNA in chromatin: Evidence for an arginine-rich histone kernel , 1976, Cell.

[6]  N. Morris.  Nucleosome structure in Aspergillus nidulans , 1976, Cell.

[7]  M. Noll.  Differences and similarities in chromatin structure of neurospora crassa and higher eucaryotes , 1976, Cell.

[8]  H. Martinson,et al.  Histone-histone interactions within chromatin. Crosslinking studies using ultraviolet light. , 1976, Biochemistry.

[9]  A. Jerzmanowski,et al.  Subunit structure of Physarum polycephalum chromatin , 1976, FEBS letters.

[10]  C. Baker,et al.  On the analysis of circular dichroic spectra of proteins. , 1976, Biochemistry.

[11]  S. Spiker.  Expression of parental histone genes in the intergeneric hybrid Triticale hexaploide , 1976, Nature.

[12]  R. Stephens,et al.  Physical Studies of Chromatin , 1976 .

[13]  P. N. Lewis.  On the native structure of the histone H3-H4 complex. , 1976, Biochemical and biophysical research communications.

[14]  J F Pardon,et al.  Low-angle neutron scattering from chromatin subunit particles. , 1975, Nucleic acids research.

[15]  K. Holde,et al.  Histone interactions and chromatin structure , 1975 .

[16]  F. V. Lente,et al.  Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt , 1975, Cell.

[17]  M. Gorovsky,et al.  Subunit structure of a naturally occurring chromatin lacking histones F1 and F3. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[18]  S. Spiker.  An evolutionary comparison of plant histones. , 1975, Biochimica et biophysica acta.

[19]  M. Bustin,et al.  Dynamic equilibrium in histone assembly: self-assembly of single histones and histone pairs. , 1975, Biochemistry.

[20]  R. Kornberg,et al.  An octamer of histones in chromatin and free in solution. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[21]  R. Chalkley.  Histone propinquity using imidoesters. , 1975, Biochemical and biophysical research communications.

[22]  H. Pollard,et al.  The presence of F3-F2a1 dimers and F1 oligomers in chromatin. , 1975, Biochemical and biophysical research communications.

[23]  F. V. Lente,et al.  Identification of specific crosslinked histones after treatment of chromatin with formaldehyde , 1975, Cell.

[24]  K. V. van Holde,et al.  Yeast chromatin subunit structure. , 1975, Science.

[25]  J. D. Engel,et al.  Subunit structure of chromatin is the same in plants and animals , 1975, Nature.

[26]  P. Nemes,et al.  The use of Coomassie Brilliant Blue G250 perchloric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels. , 1975, Analytical biochemistry.

[27]  J. Griffith.  Chromatin Structure: Deduced from a Minichromosome , 1975, Science.

[28]  H. Martinson,et al.  Histone-histone associations within chromatin. Cross-linking studies using tetranitromethane. , 1975, Biochemistry.

[29]  W. Brandt,et al.  Isolation and characterization of the histones from cycad pollen , 1975, FEBS letters.

[30]  J. A. D'Anna,et al.  A histone cross-complexing pattern. , 1974, Biochemistry.

[31]  K. V. van Holde,et al.  Electron microscopy of chromatin subunit particles. , 1974, Biochemical and biophysical research communications.

[32]  G. H. Keller,et al.  A two-subunit histone complex from calf thymus. , 1974, Biochemical and biophysical research communications.

[33]  A. Mahowald,et al.  Histones of Drosophila embryos. Electrophoretic isolation and structural studies. , 1974, The Journal of biological chemistry.

[34]  R. Kornberg,et al.  Chromatin structure; oligomers of the histones. , 1974, Science.

[35]  R. Kornberg.  Chromatin structure: a repeating unit of histones and DNA. , 1974, Science.

[36]  J. A. D'Anna,et al.  Interactions of histone LAK (f2a2) with histones KAS (f2b) and GRK (f2a1). , 1974, Biochemistry.

[37]  E. Small,et al.  A computerized fluorescence anisotropy spectrometer. , 1974, Analytical biochemistry.

[38]  D. Pallotta,et al.  Electrophoretic study of plant histones: Comparison with vertebrate histones , 1974 .

[39]  R. Chalkley,et al.  A new method for fractionating histones for physical and chemical studies. , 1974, Biochemistry.

[40]  Donald E. Olins,et al.  Spheroid Chromatin Units (ν Bodies) , 1974, Science.

[41]  M. Smerdon,et al.  The effect of temperature on histone GRK aggregation. , 1973, Biochemical and biophysical research communications.

[42]  D. E. Olins,et al.  GLUTARALDEHYDE FIXATION OF ISOLATED EUCARYOTIC NUCLEI , 1973, The Journal of cell biology.

[43]  Emil L. Smith,et al.  Histone III V. THE AMINO ACID SEQUENCE OF PEA EMBRYO HISTONE III , 1973 .

[44]  M. Roth,et al.  Column chromatography of amino acids with fluorescence detection. , 1973, Journal of chromatography.

[45]  J. A. D'Anna,et al.  A complex of histones IIb2 and IV. , 1973, Biochemistry.

[46]  S. Spiker,et al.  Constancy of wheat histones during development , 1973, Planta.

[47]  M. Olson,et al.  Amino acid sequence of the center of the arginine-lysine-rich histone from calf thymus. The total sequence. , 1972, The Journal of biological chemistry.

[48]  R. Chalkley,et al.  A modified procedure for fractionating histones. , 1972, The Biochemical journal.

[49]  H. Hayashi,et al.  Calf thymus lysine- and serine-rich histone. 3. Complete amino acid sequence and its implication for interactions of histones with DNA. , 1972, Journal of biochemistry.

[50]  R. Chalkley,et al.  The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method. , 1971, The Journal of biological chemistry.

[51]  S. Spiker.  Electrophoretic Analysis of Histones from Gibberellic Acid-treated Dwarf Peas. , 1971, Plant physiology.

[52]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[53]  R. Chalkley,et al.  Constant electrophoretic mobility of the cysteine-containing histone in plants and animals. , 1970, Biochimica et biophysica acta.

[54]  E. L. Smith,et al.  Calf and pea histone IV. 3. Complete amino acid sequence of pea seedling histone IV; comparison with the homologous calf thymus histone. , 1969, The Journal of biological chemistry.

[55]  J. Bonner,et al.  Limited molecular heterogeneity of plant histones. , 1969, Biochimica et biophysica acta.

[56]  J. Bonner,et al.  Quantitative distribution of histone components in the pea plant. , 1968, Biochemistry.

[57]  J. Bonner,et al.  On the similarity of plant and animal histones. , 1966, Biochemistry.

[58]  S. Moore,et al.  Automatic recording apparatus for use in the chromatography of amino acids. , 1958, Federation proceedings.

[59]  G. Weber.  Polarization of the fluorescence of macromolecules. I. Theory and experimental method. , 1952, The Biochemical journal.

[60]  G. Biserte,et al.  Etude de la fraction F2b des histones de thymus de veau , 1972 .

[61]  P. Meisel.  Margaret O. Dayhoff: Atlas of Protein Sequence and Structure 1969 (Volume 4) XXIV u. 361 S., 21 Ausklapptafeln, 68 Abb. und zahlreiche Tabellen. National Biomedical Research Foundation, Silver Spring/Maryland 1969. Preis $ 12,50 , 1971 .

[62]  R. Chalkley,et al.  High resolution acrylamide gel electrophoresis of histones. , 1969, Archives of biochemistry and biophysics.

[63]  M. O. Dayhoff,et al.  Atlas of protein sequence and structure , 1965 .