Pollen recognition using a multi-layer hierarchical classifier

We propose a method to recognize pollen grains using a two-stage classifier. First, texture classification categorizes the pollen grains into sub-groups. Then, a final classification of individual pollen types is done by segmenting the image int multiple layers of regions for each pollen image. The main novelty in our method is threefold: (1) Adopting two successive classification stages. (2) Combining hierarchical clustering and SVM algorithms to merge similar pollen types into sub-groups. (3) Adopting a layering approach prior to performing feature extraction. The combination of these aspects gives excellent results. We evaluated our method using 1,063 light-microscopy images of pollen grains from 30 species. The results show that: (1) the layering technique increases the classification rate by almost almost 7% over using the same features directly. (2) adopting two classification stage increases the classification rate by 6%. (3) the proposed system outperformed traditional techniques.

[1]  Matti Pietikäinen,et al.  Gray Scale and Rotation Invariant Texture Classification with Local Binary Patterns , 2000, ECCV.

[2]  Sergio Escalera,et al.  Separability of ternary codes for sparse designs of error-correcting output codes , 2009, Pattern Recognit. Lett..

[3]  Guy Lapalme,et al.  A systematic analysis of performance measures for classification tasks , 2009, Inf. Process. Manag..

[4]  Robert E. Schapire,et al.  A Brief Introduction to Boosting , 1999, IJCAI.

[5]  C. A. Hopping,et al.  Palynology and the oil industry , 1967 .

[6]  K. Holt,et al.  Principles and methods for automated palynology. , 2014, The New phytologist.

[7]  Jesús B. Alonso,et al.  Features extraction techniques for pollen grain classification , 2015, Neurocomputing.

[8]  Leo Breiman,et al.  Bagging Predictors , 1996, Machine Learning.

[9]  Yulia Arzhaeva,et al.  A comparison of classification algorithms within the Classifynder pollen imaging system , 2013 .

[10]  Corinna Cortes,et al.  Support-Vector Networks , 1995, Machine Learning.

[11]  Gabriel Cristóbal,et al.  Automated pollen identification using microscopic imaging and texture analysis. , 2015, Micron.

[12]  J. R. Flenley,et al.  Towards automation of palynology 2: the use of texture measures and neural network analysis for automated identification of optical images of pollen grains , 2004 .

[13]  Ludmila I. Kuncheva,et al.  Relationships between combination methods and measures of diversity in combining classifiers , 2002, Inf. Fusion.

[14]  E. Cernadas,et al.  Automatic detection and classification of grains of pollen based on shape and texture , 2006, IEEE Transactions on Systems, Man, and Cybernetics, Part C (Applications and Reviews).

[15]  Andrew Zisserman,et al.  A Statistical Approach to Texture Classification from Single Images , 2004, International Journal of Computer Vision.

[16]  David H. Wolpert,et al.  Stacked generalization , 1992, Neural Networks.

[17]  Ariadne Barbosa Gonçalves,et al.  Application of wavelet transform in the classification of pollen grains , 2014 .

[18]  J. R. Flenley,et al.  Towards automation of palynology 1: analysis of pollen shape and ornamentation using simple geometric measures, derived from scanning electron microscope images , 2004 .

[19]  Agma J. M. Traina,et al.  An Efficient Algorithm for Fractal Analysis of Textures , 2012, 2012 25th SIBGRAPI Conference on Graphics, Patterns and Images.

[20]  Leo Breiman,et al.  Random Forests , 2001, Machine Learning.

[21]  Daniel Müllner,et al.  Modern hierarchical, agglomerative clustering algorithms , 2011, ArXiv.

[22]  V. Bryant,et al.  Forensic palynology: why do it and how it works. , 2006, Forensic science international.

[23]  Thomas G. Dietterich Multiple Classifier Systems , 2000, Lecture Notes in Computer Science.