Inhibition of dehydration-induced fusion between liposomal membranes by carbohydrates as measured by fluorescence energy transfer.

The relative abilities of a number of naturally occurring carbohydrates to inhibit dehydration-induced fusion between palmitoyloleoylphosphatidylcholine:phosphatidylserine (85:15) large unilamellar vesicles have been studied. Fusion events were quantified using a fluorescence resonance energy transfer technique. Trehalose was most effective at inhibiting fusion (0.4 g/trehalose/g lipid showed 30% probe intermixing), followed by maltose (60% intermixing), fructose (60%), sucrose (70%), glucose (80%), cellobiose, glycerol, raffinose, and myo-inositol (90%). The relative abilities of these carbohydrates to inhibit fusion correlate directly with their abilities to interact with phospholipids, maintain bilayer fluidity, and preserve biological membranes. The results are discussed in relation to the crystalline structure of the carbohydrates and their possible influence on level of interaction with phosphate head groups.

[1]  H. A. Levy,et al.  Further refinement of the structure of sucrose based on neutron‐diffraction data , 1973 .

[2]  K. Gekko,et al.  Thermodynamic and kinetic examination of protein stabilization by glycerol. , 1981, Biochemistry.

[3]  K. Bean,et al.  Selected lipid monolayers on aqueous-glycerol and aqueous-urea substrates. , 1972, Biochimica et biophysica acta.

[4]  E. Ingenito,et al.  Stabilization of liposome bilayers to freezing and thawing: effects of cryoprotective agents and membrane proteins. , 1980, Cryobiology.

[5]  J. Crowe,et al.  Interaction of carbohydrates with dry dipalmitoylphosphatidylcholine. , 1985, Archives of biochemistry and biophysics.

[6]  J. Crowe,et al.  Effects of carbohydrates on membrane stability at low water activities. , 1984, Biochimica et biophysica acta.

[7]  G. A. Jeffrey,et al.  A neutron diffraction refinement of the crystal structure of β-maltose monohydrate , 1977 .

[8]  William N. Lipscomb,et al.  The crystal and molecular structure of cellobiose , 1961 .

[9]  K. Gekko,et al.  Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures. , 1981, Biochemistry.

[10]  A S Rudolph,et al.  Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline. , 1985, Cryobiology.

[11]  D. Hoekstra,et al.  Use of resonance energy transfer to monitor membrane fusion. , 1981, Biochemistry.

[12]  J. Crowe,et al.  Interactions of phospholipid monolayers with carbohydrates. , 1984, Biochimica et biophysica acta.

[13]  H. Berman.  The crystal structure of a trisaccharide, raffinose pentahydrate. , 1970, Acta crystallographica. Section B: Structural crystallography and crystal chemistry.

[14]  J. Lucy,et al.  Interactions of water‐soluble fusogens with phospholipids in monolayers , 1978, FEBS letters.

[15]  J. Lucy,et al.  Poly(ethylene glycol), surface potential and cell fusion. , 1976, The Biochemical journal.

[16]  P. Quinn,et al.  A lipid-phase separation model of low-temperature damage to biological membranes. , 1985, Cryobiology.

[17]  J. Crowe,et al.  Degradation of functional integrity during long-term storage of a freeze-dried biological membrane. , 1985, Cryobiology.

[18]  J. Crowe,et al.  Induction of anhydrobiosis: membrane changes during drying. , 1982, Cryobiology.

[19]  J. Crowe,et al.  Anhydrobiosis in nematodes: Carbohydrate and lipid metabolism during dehydration , 1975 .

[20]  D. Rohrer,et al.  The crystal structure of α,α-trehalose dihydrate from three independent X-ray determinations , 1972 .

[21]  J. Crowe,et al.  Hydration-dependent hexagonal phase lipid in a biological membrane. , 1982, Archives of biochemistry and biophysics.

[22]  P. Cullis,et al.  Effects of fusogenic agent on membrane structure of erythrocyte ghosts and the mechanism of membrane fusion , 1978, Nature.

[23]  J. Crowe,et al.  Preservation of structural and functional activity in lyophilized sarcoplasmic reticulum. , 1983, Archives of biochemistry and biophysics.

[24]  J. Crowe,et al.  Infrared spectroscopic studies on interactions of water and carbohydrates with a biological membrane. , 1984, Archives of biochemistry and biophysics.

[25]  D. C. Fries,et al.  Structural chemistry of carbohydrates. III. Crystal and molecular structure of 4-O-β-d-galactopyranosyl-α-d-glucopyranose monohydrate (α-lactose monohydrate) , 1971 .

[26]  D. Cadenhead,et al.  Observations and implications of glycerol-monomolecular film interactions. , 1969, Biochimica et Biophysica Acta.

[27]  U. Pick,et al.  Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures. , 1981, Archives of biochemistry and biophysics.

[28]  P. C. Hinkle,et al.  Reconstitution and purification of the D-glucose transporter from human erythrocytes. , 1977, The Journal of biological chemistry.

[29]  D. Deamer,et al.  Fusion competence of phosphatidylserine-containing liposomes quantitatively measured by a fluorescence resonance energy transfer assay. , 1981, Archives of biochemistry and biophysics.

[30]  J. Crowe,et al.  Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose , 1984, Science.

[31]  T. Taga,et al.  The crystal and molecular structure of trehalose dihydrate , 1972 .

[32]  R. H. Marchessault,et al.  Crystal and molecular structure of maltose monohydrate , 1970 .