An experimental method to determine enzyme particle emission rate in workplace

The enzyme preparation industry has been developing rapidly in recent years whose products, such as food and detergents, benefit human life in many ways. However, enzyme particles emitted during feed-in processes at workplaces can impose great risks to the workers. Local exhausts are often used to improve the indoor air quality under such conditions. To design local exhausts efficiently, information about the movement and transport of enzyme particles in workplace is required. With Computational Fluid Dynamics (CFD), the workplace enzyme particle level can be predicted from given particle source emission factors. However, no research at present relates to the determination of this emission factor with respect to particle contaminant at micro-level. We used Elutriation Dust Column (EDC) experimental method to determine enzyme particle emission factors at workplace. The principle and operation of EDC were described in detail. By introducing the particle source emission factor obtained from EDC experiment into CFD calculation, enzyme concentration level was calculated for a workplace having one feed-in machine operated intermittently. Good agreements were observed between the calculated enzyme particle concentration distributions and onsite collected sample results. It concludes that EDC can be used to predict particle emission rate in the feed-in operation and may offer a way to predict enzyme particle emission rate in similar feed-in operation processes.

[1]  Qiang Wang,et al.  Research and application of biotechnology in textile industries in China , 2007 .

[2]  O. Tupasela,et al.  Risk of enzyme allergy in the detergent industry , 2000, Occupational and environmental medicine.

[3]  David S. Ensor,et al.  Airborne particle sizes and sources found in indoor air , 1990 .

[4]  L. Morawska,et al.  Droplet fate in indoor environments, or can we prevent the spread of infection? , 2006, Indoor air.

[5]  Bruce R. White,et al.  Estimating fugitive dust emission rates using an environmental boundary layer wind tunnel , 2006 .

[6]  J. Walton,et al.  Determination of PM2.5 sources using time-resolved integrated source and receptor models. , 2006, Chemosphere.

[7]  D. Little,et al.  Respiratory disease in industry due to B. subtilis enzyme preparations. , 1973, Canadian Medical Association journal.

[8]  W. Busey,et al.  Respiratory toxicity of enzyme detergent dust. , 1978, Toxicology and applied pharmacology.

[9]  Elisabeth Mundt,et al.  Non-buoyant pollutant sources and particles in displacement ventilation , 2001 .

[10]  Wei-Zhen Lu,et al.  Numerical analysis of indoor aerosol particle deposition and distribution in two-zone ventilation system , 1996 .

[11]  B. Lighthart,et al.  Estimation of viable airborne microbes downwind from a point source , 1976, Applied and environmental microbiology.

[12]  P. Nicholson,et al.  Enzyme exposure, smoking and lung function in employees in the detergent industry over 20 years. Medical Subcommittee of the UK Soap and Detergent Industry Association. , 1997, Occupational medicine.

[13]  F. Ladeinde,et al.  CFD applications in the HVAC and R industry , 1997 .

[14]  Bin Zhao,et al.  Numerical study of the transport of droplets or particles generated by respiratory system indoors , 2004, Building and Environment.

[15]  F. Hargreave,et al.  Allergic reactions of the lungs to enzymes of Bacillus subtilis. , 1969, Lancet.

[16]  Saffa Riffat,et al.  Modelling and measurement of airflow and aerosol particle distribution in a ventilated two-zone chamber , 1996 .

[17]  Robert Gehrig,et al.  Particle emissions of a railway line determined by detailed single particle analysis , 2006 .

[18]  Bin Zhao,et al.  Comparison of indoor aerosol particle concentration and deposition in different ventilated rooms by numerical method , 2004 .

[19]  M. Sommerfeld,et al.  Multiphase Flows with Droplets and Particles , 2011 .

[20]  Richard Washington,et al.  North African dust emissions and transport , 2006 .

[21]  A. Dell'Acqua,et al.  Aerosol studies during the ESCOMPTE experiment: an overview , 2005 .

[22]  Mitzi Waltz,et al.  Webster's New World Medical Dictionary , 2000 .

[23]  Jonathan Y. Richmond,et al.  Biosafety in microbiological and biomedical laboratories , 1999 .

[24]  Kikuo Okuyama,et al.  Numerical Simulation and Experiment on the Transport of Fine Particles in a Ventilated Room , 1996 .