Lymphoproliferative disease in a cotton‐top marmoset after inoculation with infectious mononucleosis‐derived Epstein‐Barr virus

Injection of concentrated EBV derived from cells of the Kaplan line of infectious mononucleosis (IM) origin resulted in malignant lymphoproliferation in one out of three cotton‐top marmosets 6 weeks after inoculation. Two additional animals receiving the same isolate after incubation with an antibody‐containing human serum did not develop tumors. Inoculation of concentrated virus derived from the P3HR‐1 line of Burkitt origin did not lead to lymphoproliferation in five marmosets. Three of these received non‐neutralized, and two received neutralized P3HR‐1 virus. The tumor obtained with the Kaplan isolate revealed characteristics of a lymphosarcoma. It contained EBV‐specific DNA. In addition, EBV‐synthesizing lymphoblastoid lines were established from a tumorous lymph‐node, as well as from the spleen of the diseased marmoset. Virus recovered from these lines transformed lymphocytes derived from spleens of healthy marmosets. The tumor‐bearing animal developed low levels of anti‐VCA antibodies during the course of tumor growth. These data demonstrate the oncogenic potential of EBV directly derived from cells of IM origin.

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