Hog Cholera: I. Investigation of the Agar Double-Diffusion Precipitation Test for The Detection of the Virus in Swine Tissue.

The agar double-diffusion precipitation test was investigated to detect hog cholera virus in splenic and pancreatic tissues of swine. Special attention was given to the conditions influencing the sensitivity and specificity of the test. These studies emphasize the strict techniques and methods required in the test in order to detect specific antigen - antibody reaction. Absorption studies performed on serum fractions prepared by DEAE cellulose chromatography and studied electrophoretically, indicated the specific reaction was given by fractions, migrating in the gamma-globulin region and was absorbed by infected but not by normal spleens. The sensitivity of the test is dependent to a great extent on the successful liberation of the viral antigen from the tissue.

[1]  W. Mengeling,et al.  Identification of Hog Cholera Viral Antigen by Immunofluorescence. Application as a Diagnostic and Assay Method. , 1963, Canadian journal of comparative medicine and veterinary science.

[2]  E. C. Pirtle.  Precipitation Reactions in Agar Between Swine Serum and Homologous Pancreas Extracts. , 1963, Canadian journal of comparative medicine and veterinary science.

[3]  G. A. Young,et al.  A Hog Cholera Virus-Fluorescent Antibody System. Its Potential Use in Study of Embryonic Infection , 1963, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[4]  D. Segre.  Detection of hog cholera virus by a hemagglutination test. , 1962, American journal of veterinary research.

[5]  A. Robertson,et al.  Fluorescein-Labelled Antibody Technique for the Demonstration of Leptospira Pomona. , 1961, Canadian journal of comparative medicine and veterinary science.

[6]  T. Shimizu,et al.  A new in vitro method (END) for detection and measurement of hog cholera virus and its antibody by means of effect of HC virus on Newcastle disease virus in swine tissue culture. I. Establishment of standard procedure. , 1961, Journal of immunology.

[7]  Millian Sj,et al.  Application of the conglutination complement absorption test to detect hog cholera antibodies. I. The technique. , 1961 .

[8]  J. Gillespie,et al.  Propagation of Hog Cholera Virus in Tissue Culture.∗ , 1960, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[9]  A. Kawakubo,et al.  Propagation of hog cholera virus in tissue culture and its application to vaccine. I. Propagation of the virus in tissue culture. , 1960, American journal of veterinary research.

[10]  D. Segre.  A new serologic test for the detection of viral antigens: its application to the viruses of hog cholera and vesicular stomatitis. , 1957, Journal of immunology.

[11]  A. W. McClurkin.  Use of the agar diffusion precipitation test in the diagnosis of hog cholera. , 1963, Canadian journal of comparative medicine and veterinary science.

[12]  S. Millian,et al.  Application of the conglutination complement absorption test to detect hog cholera antibodies. I. The technique. , 1961, American journal of veterinary research.

[13]  J. Darbyshire.  A serological relationship between swine fever and mucosal disease of cattle. , 1960 .

[14]  A. Sobko,et al.  Agar gel diffusion test for the diagnosis of swine fever. , 1960 .

[15]  W. Mansi.  The study of some viruses by the plate gel diffusion precipitin test. , 1957, Journal of comparative pathology.