Ultrastructure of mouse vallate taste buds: II. Cell types and cell lineage

The lifespan of cells in the mouse taste bud was examined with high‐voltage electron microscopic (HVEM) autoradiography (ARG) after giving a single injection of 3H‐thymidine. Animals were killed at 1 hour, 6 hours, 12 hours, 24 hours, and then daily up through 10 days postinjection. Lingual tissues were prepared for HVEM ARG so that we could identify and characterize labeled cells. Four categories of taste cells were identified: basal, dark, intermediate, and light cells. Basal cells were polygonal cells located near the basolateral sides of the taste buds and were characterized primarily by the presence of filaments attached to the nuclear envelope. Dark and light cells had the typical features described by previous authors. Intermediate cells had features in between those of dark and light cells.

[1]  J. C. Kinnamon,et al.  Ultrastructure of mouse vallate taste buds. I. Taste cells and their associated synapses , 1985, The Journal of comparative neurology.

[2]  A. Farbman,et al.  Structure of taste buds in foliate papillae of the rhesus monkey, Macaca mulatta. , 1985, The American journal of anatomy.

[3]  B. Munger,et al.  Fetal development of primate chemosensory corpuscles. II. Synaptic relationships in early gestation , 1983, The Journal of comparative neurology.

[4]  A. Farbman.  RENEWAL OF TASTE BUD CELLS IN RAT CIRCUMVALLATE PAPILLAE , 1980, Cell and tissue kinetics.

[5]  B. Munger,et al.  The cytologic composition of primate laryngeal chemosensory corpuscles. , 1980, The American journal of anatomy.

[6]  M. Williams.  Autoradiography and immunocytochemistry , 1977 .

[7]  L. Guth.  Degeneration and Regeneration of Taste Buds , 1971 .

[8]  A. Farbman.  Development of the Taste Bud , 1971 .

[9]  R. G. Murray,et al.  Relations and possible significance of taste bud cells. , 1971, Contributions to sensory physiology.

[10]  S. Fujimoto,et al.  Fine structure of degeneration and regeneration in denervated rabbit vallate taste buds , 1970, The Anatomical record.

[11]  S. Fujimoto,et al.  Fine structure of gustatory cells in rabbit taste buds. , 1969, Journal of ultrastructure research.

[12]  A. D. Conger,et al.  Radiation and aging effect on taste structure and function. , 1969, Radiation research.

[13]  R. G. Murray,et al.  Fine structure of taste buds of rabbit foliate papillae. , 1967, Journal of ultrastructure research.

[14]  L. M. Beidler,et al.  RENEWAL OF CELLS WITHIN TASTE BUDS , 1965, The Journal of cell biology.

[15]  A. Farbman.  FINE STRUCTURE OF THE TASTE BUD. , 1965, Journal of ultrastructure research.

[16]  A. Farbman.  ELECTRON MICROSCOPE STUDY OF THE DEVELOPING TASTE BUD IN RAT FUNGIFORM PAPILLA. , 1965, Developmental biology.

[17]  L. Guth.  Histological changes following partial denervation of the circumvallate papilla of the rat , 1963 .

[18]  A. D. Lorenzo.  STUDIES ON THE ULTRASTRUCTURE AND HISTOPHYSIOLOGY OF CELL MEMBRANES, NERVE FIBERS AND SYNAPTIC JUNCTIONS IN CHEMORECEPTORS* , 1963 .

[19]  Seymour Geisser,et al.  Statistical Principles in Experimental Design , 1963 .

[20]  L. Guth.  Taste buds on the cat's circumvallate papilla after reinnervation by glossopharyngeal, vagus, and hypoglossal nerves , 1958, The Anatomical record.

[21]  L. Guth.  The effects of glossopharyngeal nerve transection on the circumvallate papilla of the rat , 1957, The Anatomical record.

[22]  H. Engström,et al.  XXX The Fine Structure of Taste Buds and Taste Fibres , 1956, The Annals of otology, rhinology, and laryngology.

[23]  R. May.  The relation of nerves to degenerating and regenerating taste buds , 1925 .

[24]  M. Heidenhain.  Über die Sinnesfelder und die Geschmacksknospen der Papilla foliata des Kaninchens , 1914 .