Femtogram detection of cytokines in a direct dot-blot assay using SERS microspectroscopy and hydrophilically stabilized Au-Ag nanoshells.

Rapid parallel detection of two cytokines (IL-6 and IL-8) with femtogram sensitivity in a simple direct dot-blot assay is demonstrated. The microspectroscopic SERS acquisition scheme employs rationally designed, hydrophilically stabilized Au-Ag nanoshells as SERS labels, which are optimized for signal enhancement upon red laser excitation.

[1]  Yuling Wang,et al.  Rational design and synthesis of SERS labels. , 2013, The Analyst.

[2]  Yuling Wang,et al.  Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells. , 2013, The Analyst.

[3]  Zhenxin Wang,et al.  Gold nanoparticle based dot-blot immunoassay for sensitively detecting Alzheimer's disease related β-amyloid peptide. , 2012, Chemical communications.

[4]  Boris N. Khlebtsov,et al.  Multiplexed dot immunoassay using Ag nanocubes, Au/Ag alloy nanoparticles, and Au/Ag nanocages , 2012, Nano Research.

[5]  Jaebum Choo,et al.  Simultaneous immunoassay for the detection of two lung cancer markers using functionalized SERS nanoprobes. , 2011, Chemical communications.

[6]  Yong-Kweon Kim,et al.  Surface-enhanced Raman scattering-active nanostructures and strategies for bioassays. , 2011, Nanomedicine.

[7]  I. Petersen,et al.  Immuno-surface-enhanced coherent anti-stokes Raman scattering microscopy: immunohistochemistry with target-specific metallic nanoprobes and nonlinear Raman microscopy. , 2011, Analytical chemistry.

[8]  Shiv K. Sharma,et al.  Nitrocellulose-based SERS immunosensor for detection of biological molecules , 2011, Defense + Commercial Sensing.

[9]  T. Keiderling,et al.  SERS biodetection using gold-silica nanoshells and nitrocellulose membranes. , 2011, Analytical chemistry.

[10]  Marc D Porter,et al.  Detection of the potential pancreatic cancer marker MUC4 in serum using surface-enhanced Raman scattering. , 2011, Analytical chemistry.

[11]  Jaebum Choo,et al.  Optoelectrofluidic sandwich immunoassays for detection of human tumor marker using surface-enhanced Raman scattering. , 2010, Analytical chemistry.

[12]  I. Boyaci,et al.  A highly sensitive detection platform based on surface-enhanced Raman scattering for Escherichia coli enumeration , 2010, Analytical and bioanalytical chemistry.

[13]  C. Schmuck,et al.  Water soluble SERS labels comprising a SAM with dual spacers for controlled bioconjugation. , 2009, Physical chemistry chemical physics : PCCP.

[14]  Sebastian Schlücker,et al.  SERS microscopy: nanoparticle probes and biomedical applications. , 2009, Chemphyschem : a European journal of chemical physics and physical chemistry.

[15]  Sebastian Schlücker,et al.  Optical properties and SERS efficiency of tunable gold/silver nanoshells , 2009 .

[16]  Alexander Marx,et al.  SERS labels for red laser excitation: silica-encapsulated SAMs on tunable gold/silver nanoshells. , 2009, Angewandte Chemie.

[17]  G. Kuchel,et al.  ELISA and multiplex technologies for cytokine measurement in inflammation and aging research. , 2008, The journals of gerontology. Series A, Biological sciences and medical sciences.

[18]  Marc D Porter,et al.  SERS as a bioassay platform: fundamentals, design, and applications. , 2008, Chemical Society reviews.

[19]  Shaojun Dong,et al.  Ultrasensitive colorimetric detection of protein by aptamer-Au nanoparticles conjugates based on a dot-blot assay. , 2008, Chemical communications.

[20]  Yukihiro Ozaki,et al.  Analytical technique for label-free multi-protein detection based on Western blot and surface-enhanced Raman scattering. , 2008, Analytical chemistry.

[21]  Jackie Y. Ying,et al.  Synthesis of Functionalized Au Nanoparticles for Protein Detection , 2008 .

[22]  Yong-Kweon Kim,et al.  Surface-enhanced Raman spectroscopic-encoded beads for multiplex immunoassay. , 2007, Journal of combinatorial chemistry.

[23]  Shao-Yi Hou,et al.  Development of zeptomole and attomolar detection sensitivity of biotin-peptide using a dot-blot gold nanoparticle immunoassay. , 2007, Analytical chemistry.

[24]  Anand Gole,et al.  Surface-enhanced Raman spectroscopy of self-assembled monolayers: sandwich architecture and nanoparticle shape dependence. , 2005, Analytical chemistry.

[25]  Yukihiro Ozaki,et al.  Immunoassay using probe-labelling immunogold nanoparticles with silver staining enhancement via surface-enhanced Raman scattering. , 2004, The Analyst.

[26]  M. Porter,et al.  Femtomolar detection of prostate-specific antigen: an immunoassay based on surface-enhanced Raman scattering and immunogold labels. , 2003, Analytical chemistry.

[27]  Sebastian Schlücker,et al.  Raman microspectroscopy: a comparison of point, line, and wide-field imaging methodologies. , 2003, Analytical chemistry.

[28]  Younan Xia,et al.  Increased sensitivity of surface plasmon resonance of gold nanoshells compared to that of gold solid colloids in response to environmental changes. , 2002, Analytical chemistry.

[29]  Younan Xia,et al.  Template-Engaged Replacement Reaction: A One-Step Approach to the Large-Scale Synthesis of Metal Nanostructures with Hollow Interiors , 2002 .

[30]  R. G. Freeman,et al.  Ag-Clad Au Nanoparticles: Novel Aggregation, Optical, and Surface-Enhanced Raman Scattering Properties , 1996 .

[31]  T. Whiteside.  Cytokine measurements and interpretation of cytokine assays in human disease , 1994, Journal of Clinical Immunology.

[32]  K. Matsushima,et al.  Essential involvement of interleukin‐8 (IL‐8) in acute inflammation , 1994, Journal of leukocyte biology.

[33]  M. Wadhwa,et al.  Detection and measurement of cytokines. , 1992, Blood reviews.

[34]  A. Frumkin,et al.  Die anwendung der rotierenden scheibenelektrode mit einem ringe zur untersuchung von zwischenprodukten elektrochemischer reaktionen , 1959 .

[35]  Peter Lipsky,et al.  Cytokines and autoimmunity , 2002, Nature Reviews Immunology.