The effects of cholera toxin, pertussis toxin, sodium fluoride and alpha-interferon on prostaglandin production by the guinea-pig endometrium.

The outputs of prostaglandin (PG) F-2 alpha, 6-keto-PGF-1 alpha and PGE-2 from Day-7 and Day-15 guinea-pig endometrium were neither stimulated nor inhibited by cholera toxin and pertussis toxin. This indicates that PG synthesis by guinea-pig endometrium is not controlled by toxin-sensitive G-proteins. Short-term treatment of guinea-pig endometrium in culture with sodium fluoride stimulated PG output, suggesting that endometrial PG synthesis may be regulated by a fluoride-sensitive G-protein. Long-term treatment of guinea-pig endometrium in culture with sodium fluoride inhibited endometrial PG synthesis, and this was due to an inhibition of endometrial protein synthesis. Human alpha-interferon had no inhibitory effect on the outputs of PGF-2 alpha, 6-keto-PGF-1 alpha and PGE-2 from Day-15 guinea-pig endometrium in culture. It appears that the anti-luteolytic factor secreted by guinea-pig conceptus is not an alpha-interferon and is therefore probably different from ovine trophoblast protein-1.

[1]  S. Riley,et al.  Is protein synthesis necessary for prostaglandin production by guinea-pig endometrium? , 1989, Journal of reproduction and fertility.

[2]  J. Axelrod,et al.  Receptor-mediated activation of phospholipase A2 via GTP-binding proteins: arachidonic acid and its metabolites as second messengers , 1988, Trends in Neurosciences.

[3]  M. Kester,et al.  Involvement of a pertussis toxin-sensitive G-protein-coupled phospholipase A2 in lipopolysaccharide-stimulated prostaglandin E2 synthesis in cultured rat mesangial cells. , 1988, Biochimica et biophysica acta.

[4]  J. Godkin,et al.  Interferon-alpha mimics effects of ovine trophoblast protein 1 on prostaglandin and protein secretion by ovine endometrial cells in vitro. , 1988, The Journal of endocrinology.

[5]  S. Nakashima,et al.  Stimulation of arachidonic acid release by guanine nucleotide in saponin-permeabilized neutrophils: evidence for involvement of GTP-binding protein in phospholipase A2 activation. , 1988, Archives of biochemistry and biophysics.

[6]  P. Dandona,et al.  Fluoride stimulates in vitro vascular prostacyclin synthesis: interrelationship of G proteins and protein kinase C. , 1988, European journal of pharmacology.

[7]  J. Huet,et al.  High homology between a trophoblastic protein (trophoblastin) isolated from ovine embryo and α‐interferons , 1988, FEBS letters.

[8]  J. Axelrod,et al.  Cholera toxin and pertussis toxin stimulate prostaglandin E2 synthesis in a murine macrophage cell line. , 1988, The Journal of pharmacology and experimental therapeutics.

[9]  P. Barker,et al.  Interferon sequence homology and receptor binding activity of ovine trophoblast antiluteolytic protein. , 1987, The Journal of endocrinology.

[10]  S. Riley,et al.  Effects of oestradiol, progesterone, hydrocortisone and oxytocin on prostaglandin output from the guinea-pig endometrium maintained in tissue culture. , 1987, Prostaglandins.

[11]  C. Erneux,et al.  Dual role of GTP-binding proteins in the control of endothelial prostacyclin. , 1987, Biochemical and biophysical research communications.

[12]  J. Axelrod,et al.  Dissociation of bradykinin-induced prostaglandin formation from phosphatidylinositol turnover in Swiss 3T3 fibroblasts: evidence for G protein regulation of phospholipase A2. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[13]  S. Riley,et al.  Prostaglandin production by the guinea-pig endometrium: is calcium necessary? , 1987, The Journal of endocrinology.

[14]  N. Poyser,et al.  Further studies on the control of arachidonic acid turnover in guinea-pig endometrium in relation to prostaglandin production. , 1984, Prostaglandins, leukotrienes, and medicine.

[15]  N. Poyser.  Prostaglandin production by the early pregnant guinea-pig uterus in relation to implantation and luteal maintenance, and the effect of oestradiol. , 1984, Journal of reproduction and fertility.

[16]  N. Poyser,et al.  Estimation of phospholipase A2 activity in guinea-pig endometrium on days 7 and 16 of the estrous cycle. , 1983, Prostaglandins, leukotrienes, and medicine.

[17]  N. Poyser.  Effect of treating ovariectomized guinea-pigs with estradiol and progesterone on basal and A23187-stimulated release of prostaglandins from the uterus superfused in vitro. , 1983, Prostaglandins, leukotrienes, and medicine.

[18]  H. Leaver,et al.  Arachidonic acid uptake into and release from guinea-pig endometrium in vitro on days 7 and 15 of the oestrous cycle. , 1983, Prostaglandins, leukotrienes, and medicine.

[19]  H. Leaver,et al.  Distribution of arachidonic acid and other fatty acids in the lipids of guinea-pig uterus and plasma in relation to uterine prostaglandin synthesis. , 1981, Journal of reproduction and fertility.

[20]  N. Poyser.  Effect of actinomycin D on uterine prostaglandin production and oestrous cycle length in guinea-pigs. , 1979, Journal of reproduction and fertility.

[21]  E. Horton,et al.  Uterine luteolytic hormone: a physiological role for prostaglandin F2alpha. , 1976, Physiological reviews.

[22]  N. Poyser.  Production of prostaglandins by the guinea-pig uterus. , 1972, The Journal of endocrinology.

[23]  E. Horton,et al.  The release of prostaglandins and progestin into the utero‐ovarian venous blood of guinea‐pigs during the oestrous cycle and following oestrogen treatment , 1972, The Journal of physiology.

[24]  A. Flint,et al.  Sheep antiluteolytic interferon: cDNA sequence and analysis of mRNA levels. , 1989, Journal of molecular endocrinology.

[25]  R. Burgoyne,et al.  Receptor-activation of phospholipase A2 in cellular signalling , 1987 .

[26]  R. Roberts,et al.  Interferon-like sequence of ovine trophoblast protein secreted by embryonic trophectoderm , 1987, Nature.

[27]  R. Roberts,et al.  Proteins secreted by the sheep conceptus suppress induction of uterine prostaglandin F-2 alpha release by oestradiol and oxytocin. , 1986, Journal of reproduction and fertility.

[28]  H. Behrman,et al.  Prostaglandins in reproduction. , 1974, Archives of internal medicine.

[29]  J. Axelrod,et al.  Dissociation of bradykinin-induced prostaglandin formation from phosphatidylinositol turnover in Swiss 3 T 3 fibroblasts : Evidence for G protein regulation of phospholipase A 2 ( arachidonic acid / glucocorticoids / protein kinase C ) , 2022 .