Activity Changes During Stress Erythropoiesis

Many features of.normal and abnormal erythropoiesis have been described in the inbred mouse, but no systematic studies of enzymatic activities in mouse erythrocytes have been reported. The activities of 14 enzymes of glycolysis and the hexose monophosphate shunt have been measured in mouse erythrocytes formed under normal conditions and during hemopoietic stress induced by phenylhydrazine treatment. The responses of inbred strains A/J and C57L/J were compared. Innormal cells of each strain the activity of hexokinase was lowest, that of triosephosphate isomerase the highest, and the rank order of other enzymatic activities in the mouse was similar to that reported for the human #{233}rythrocyte. All enzymatic activities increased during the reticulocytosis accompanying stress eryth ropoiesis, with phosphofructokinase, aldolase, phos