Purification , Characterization and Immunological Properties of the Serotype-specific Capsular Polysaccharide of Pasteurella haemolytica ( Serotype Al ) Organisms

The serotype-specific capsular polysaccharide from two strains of Pasteurella haemolytica serotype A1 organisms was purified and characterized by chemical analysis and NMR spectroscopy. The polymer has the structure +3)-0-(2-acetamido-2-deoxy-4-0-acetyl-~-~mannopyranosyluronic acid)-( 1 +4)-0-(2-acetamido-2-deoxy-/?-~-mannopyranose)-( 1 + . The polysaccharide was immunogenic (able to evoke production of antibodies) for sheep but not for rabbits. Immuno electron-microscopy studies using the Protein A-gold technique showed the polysaccharide to be peripherally located on the bacterial surface. Reduction, oxidation and de0-acetylation of the polymer did not appear to alter its immunological precipitability with specific antiserum, but all three treatments destroyed its ability to adhere to sheep erythrocytes at neutral pH. De-N-acetylation of the polymer destroyed both immunological precipitability and erythrocyte adherence.

[1]  A. Goustin,et al.  Use of proton n.m.r. spectroscopy for detection of 2-acetamido-2-deoxy-d-mannose- and 2-acetamido-2-deoxy-d-mannuronate-containing carbohydrates , 1983 .

[2]  D. Carlo,et al.  The specific capsular polysaccharide of Streptococcus pneumoniae type 9V. , 1983, Canadian journal of biochemistry.

[3]  T. Mikami,et al.  Gelation of Limulus Amoebocyte Lysate by Simple Polysaccharides , 1982 .

[4]  K. Yokoyama,et al.  Distribution of mannosamine and mannosaminuronic acid among cell walls of Bacillus species , 1982, Journal of bacteriology.

[5]  P. Gorin.  Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Polysaccharides , 1981 .

[6]  P. Jansson,et al.  Structural studies of the capsular polysaccharide from Streptococcus pneumoniae type 4. , 1981, Carbohydrate research.

[7]  C. T. Bishop,et al.  Structure of the type XXXIII Streptococcuspneumoniae (pneumococcal) capsular polysaccharide , 1980 .

[8]  R. Schneerson,et al.  Structural and immunological studies of the Haemophilus influenzae type d capsular polysaccharide. , 1980, Carbohydrate research.

[9]  R. Schneerson,et al.  Structural studies of the Haemophilus influenzae type e capsular polysaccharide. , 1980, Carbohydrate research.

[10]  R. L. Smith,et al.  Quantitation of glycosaminoglycan hexosamine using 3-methyl-2-benzothiazolone hydrazone hydrochloride. , 1979, Analytical biochemistry.

[11]  Gareth A. Morris,et al.  Enhancement of nuclear magnetic resonance signals by polarization transfer , 1979 .

[12]  S. Hansen.  Thin-layer chromatographic method for the identification of mono-, di- and trisaccharides , 1975 .

[13]  C. P. Kenny,et al.  Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. , 1975, The Journal of biological chemistry.

[14]  J. Hash,et al.  Surface Polysaccharide from Staphylococcus aureus M That Contains Taurine, d-Aminogalacturonic Acid, and d-Fucosamine , 1974, Journal of bacteriology.

[15]  D. Bundle,et al.  The Carbon-13 Nuclear Magnetic Resonance Spectra of 2-Acetamido-2-deoxy-D-hexoses and some Specifically Deuteriated, O-Acetylated, and Phosphorylated Derivatives , 1973 .

[16]  D. Horton,et al.  The nitrous acid deamination of 2-amino-2-deoxy-d-mannose hydrochloride to d-glucose , 1972 .

[17]  J. T. Park,et al.  Chemical Characterization of a New Surface Antigenic Polysaccharide from a Mutant of Staphylococcus aureus , 1971, Journal of bacteriology.

[18]  L. Warren,et al.  The thiobarbituric acid assay of sialic acids. , 1959, The Journal of biological chemistry.

[19]  R. Jeanloz,et al.  Identification of amino sugars by paper chromatography. , 1954, Archives of biochemistry and biophysics.

[20]  S. Hestrin.  The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. , 1949, The Journal of biological chemistry.

[21]  J. Fraser,et al.  A new serotype (biotype T) of Pasteurella haemolytica. , 1982, Research in veterinary science.

[22]  B. Lindberg,et al.  Methylation analysis of complex carbohydrates: general procedure and application for sequence analysis. , 1978, Methods in enzymology.

[23]  C. Penn,et al.  Isolation of a protective, non-toxic capsular antigen from Pasteurella multocida, types B and E. , 1976, Research in veterinary science.

[24]  C. Penn,et al.  Protective antigens in bovine pasteurellosis. , 1974, Developments in biological standardization.

[25]  E. Gotschlich,et al.  The immunological responses observed in field studies in Africa with group A meningococcal vaccines. , 1971, Progress in immunobiological standardization.

[26]  R. Spiro,et al.  [1] Analysis of sugars found in glycoproteins , 1966 .

[27]  O. Westphal.  Bacterial lipopolysaccharides : extraction with phenol-water and further applications of the procedure , 1965 .

[28]  L. Svennerholm,et al.  Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. , 1957, Biochimica et Biophysica Acta.

[29]  F. Smith,et al.  COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE , 1956 .

[30]  J. H. Roe.  The determination of sugar in blood and spinal fluid with anthrone reagent. , 1955, The Journal of biological chemistry.

[31]  S. Gardell,et al.  Oxidation of Glucosamine and Galactosamine with Ninhydrin to Arabinose and Lyxose and their Identification with Paper Chromatography. , 1950 .

[32]  Z. Dische,et al.  A new specific color reaction of hexuronic acids. , 1947, The Journal of biological chemistry.