Plasma clearance of intravenously injected aspartate aminotransferase isozymes: Evidence for preferential uptake by sinusoidal liver cells

Both cytosolic (c‐AAT) and mitochondrial (m‐AAT) isozymes of aspartate aminotransferase (EC 2.6.1.1) appear in serum in some diseases including hepatobiliary dysfunction. The present study aimed at elucidation of the mechanism by which AAT isozymes are cleared from blood. Intravenous injection into rats of m‐AAT and c‐AAT purified from rat liver exhibited a biphasic clearance curve with an overall half‐life of 42 min and 4.7 hr, respectively. The tissue distribution of the radioactivity following intravenous administration of 125I‐labeled isozymes revealed that the liver is a major organ involved in plasma clearance of these isozymes. This conclusion was also supported by the significant retardation in plasma clearance of m‐AAT in hepatectomized as well as CCI4‐intoxicated rats. Furthermore, clearance rate of each AAT isozyme in an isolated perfused liver exhibited a single exponential process with the uptake rate for m‐AAT being much faster than that for c‐AAT.

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