Hydrophobic interaction chromatography.

Hydrophobic interaction chromatography (HIC) is emerging as a useful technique for the separation of biological compounds. Advances in the past two years in HIC applications, stationary phases, eluents, and theory are reviewed. Recent applications of HIC processes include analytical and semi-preparative separations of a variety of proteins, such as isolectins, hemoglobins, calmodulin, and cardiotoxins. Additionally, HIC is being employed as a tool to investigate protein properties and mechanisms. Advances in HIC stationary phases include development of non-porous, microparticulate supports as well as supports with pore sizes up to 1000 Angstroms. Studies of HIC eluents have further shown the effects of mobile phase pH, water-structuring characterization, and surface tension increments on retention. Various retention mechanisms which have been presented are reviewed; and a correlation relating resolution to column and solute parameters is presented. Protein conformational effects at specific sites have been shown to have a significant impact on retention and specific examples illustrating such effects are discussed.

[1]  T. Arakawa,et al.  Thermodynamic analysis of the effect of concentrated salts on protein interaction with hydrophobic and polysaccharide columns. , 1986, Archives of biochemistry and biophysics.

[2]  N. Seno,et al.  Separation of isolectins by high-performance hydrophobic interaction chromatography. , 1987, Journal of chromatography.

[3]  S. Berkowitz Linear multidimensional liquid chromatography in the preparative scale purification of calmodulin from brain extract. , 1987, Analytical biochemistry.

[4]  J. Porath Metal Ion — Hydrophobic, Thiophilic and II‐Electron Governed Interactions and their Application to Salt‐Promoted Protein Adsorption Chromatography , 1987 .

[5]  T. Arakawa,et al.  Preferential interactions of proteins with salts in concentrated solutions. , 1982, Biochemistry.

[6]  F. Regnier,et al.  Comparison of hydrophobic-interaction and reversed-phase chromatography of proteins. , 1984, Journal of chromatography.

[7]  F. Regnier,et al.  Solute and mobile phase contributions to retention in hydrophobic interaction chromatography of proteins. , 1986, Journal of chromatography.

[8]  P. Beaune,et al.  Hydrophobic chromatography: a one-step method for the purification of human liver microsomal epoxide hydrolase. , 1988, Journal of chromatography.

[9]  J. Porath,et al.  Sorption Effects in Gel Filtration: I. A Survey of Amino Acid Behavior on Sephadex G-10 , 1967 .

[10]  J. Wittliff,et al.  Characterization of estrogen receptors and associated protein kinase activity by high-performance hydrophobic-interaction chromatography. , 1987, Journal of chromatography.

[11]  N. Miller,et al.  Preparative Hydrophobic Interaction Chromatography of Proteins Using Ether Based Chemically Bonded Phases , 1986 .

[12]  Silica–Based Chromatography Products , 1987, Bio/Technology.

[13]  K. Adachi,et al.  Separation of asymmetrical hybrid hemoglobins by hydrophobic interaction chromatography. , 1987, Journal of chromatography.

[14]  J. Christophe,et al.  Rapid purification of Bordetella pertussis toxin by alternating affinity and hydrophobic chromatography. , 1986, Analytical biochemistry.

[15]  F. Regnier,et al.  The role of protein structure in chromatographic behavior. , 1987, Science.

[16]  B. Karger,et al.  Protein conformational effect in hydrophobic interaction chromatography , 1986 .

[17]  A. Pinsky,et al.  Isolation of a thermophilic alkaline phosphatase by either hydrophobic or procion red sepharose chromatography , 1986 .

[18]  M. Chaudhury,et al.  Determination off the Hydrophobia Interaction Energy-Application to Separation Processes , 1987 .

[19]  Y. Sano,et al.  Resolution of proteins in linear gradient elution ion-exchange and hydrophobic interaction chromatography. , 1987, Journal of chromatography.

[20]  C. Densmore,et al.  Activation of Glucocorticoid‐Type II Receptor Complexes in Brain Cytosol Leads to an Increase in Surface Hydrophobicity as Determined by Hydrophobic Interaction Chromatography , 1988, Journal of neurochemistry.

[21]  T. Kitamura,et al.  High-Performance Hydrophobic Interaction Chromatography of Proteins on TSKgel Phenyl-5PW Preparative Column , 1986 .

[22]  R. Midura,et al.  Proteoglycans: isolation and purification from tissue cultures. , 1987, Methods in Enzymology.

[23]  J. Withka,et al.  Use of high-performance size-exclusion, ion-exchange, and hydrophobic interaction chromatography for the measurement of protein conformational change and stability. , 1987, Journal of chromatography.

[24]  K. D. Collins,et al.  The systematic characterization by aqueous column chromatography of solutes which affect protein stability. , 1986, The Journal of biological chemistry.

[25]  M. Schmuck,et al.  Optimization of preparative hydrophobic interaction chromatographic purification methods. , 1986, Journal of chromatography.

[26]  C. Horváth,et al.  Salt effect on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic series. , 1977, Archives of biochemistry and biophysics.

[27]  R. Lewis,et al.  REVERSED-PHASE CHROMATOGRAPHY OF PROTEINS AND NUCLEIC ACIDS: PRACTICAL CONSIDERATIONS , 1986 .

[28]  C. L. D. Ligny,et al.  Hydrophobic interaction chromatography of simple compounds on alkyl-agaroses with different alkyl chain lengths and chain densities : Mechanism and thermodynamics , 1986 .

[29]  T. Horbett,et al.  Proteins at interfaces : physicochemical and biochemical studies , 1987 .

[30]  M. Hearn,et al.  Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography. V. Performance of non-porous monodisperse 1.5-microns bonded silicas in the separation of proteins by hydrophobic-interaction chromatography. , 1987, Journal of chromatography.

[31]  K. Unger,et al.  Non-porous microparticulate supports in high-performance liquid chromatography (HPLC) of biopolymers — concepts, realization and prospects , 1986 .

[32]  U. Hellman,et al.  Hydrophobic interaction chromatography of incompletely methylated transfer RNA from Escherichia coli on octyl-sepharose. , 1986, Journal of chromatography.

[33]  Barber We,et al.  Column liquid chromatography. , 1982, Analytical chemistry.

[34]  F. Ahmad,et al.  Thermodynamic stability of proteins in salt solutions: A comparison of the effectiveness of protein stabilizers , 1986 .

[35]  A. Louw,et al.  Reversed-phase and hydrophobic-interaction high-performance liquid chromatography of elapid cardiotoxins. , 1987, Analytical biochemistry.

[36]  K. D. Collins,et al.  The Hofmeister effect and the behaviour of water at interfaces , 1985, Quarterly Reviews of Biophysics.