Summary
Of three prototype strains of Mycoplasma bovirhinis examined for their ability to adsorb sheep erythrocytes, two (CCM M-22 and TH) showed positive haemadsorption whereas one strain (N. C. T. C. 10 118) failed to adsorb sheep erythrocytes. The original classification of strain CCM M-22 as M. bovirhinis was confirmed by the tube agglutination test with antisera of all serotypes of bovine mycoplasmas. M. bovirhinis strain CCM M-22 was also tested for its ability to adsorb other cells derived from various mammalian and avian tissues and tissue cultures.
As pneumonic lungs of calves examined in this laboratory for many years have yielded only haemadsorbing mycoplasma strains and no non-haemadsorbing strains, haemadsorption is recommended as a characteristic suitable for tentative differentiation of mycoplasmas.
Zusammenfassung
Adsorbierende und nichtadsorbierende Stamme von Mycoplasma bovirhinis
Drei als Prototypen geltende Stamme von Mycoplasma bovirhinis wurden auf ihre Fahigkeit untersucht, Schaferythrozyten zu adsorbieren. Bei 2 Stammen (CCM M-22 und TH) verliefen die Versuche positiv, beim Stamm N. C. T. C. 10 118 negativ.
Der Stamm CCM M-22 adsorbierte auserdem Zellen verschiedener Saugerorgane, Zellkulturen und bovine Spermatozoen. Der Erythrozyten-Hamadsorptionstest wird als Differenzierungsmethode fur bovine Mycoplasmastamme vorgeschlagen.
Resume
Souches adsorbantes et non adsorbantes de Mycoplasma bovirhinis
On examine trois souches de Mycoplasma bovirhinis, admises comme prototypes, quant a leur capacite d'adsorber des erythrocytes de moutons. Deux de ces souches (CCM M-22 et TH) donnent des resultats positifs et al souche N.C.T.C. 10118 des resultats negatifs.
La souche CCM M-22 adsorbe en outre des cellules de differents organes de mammiferes, des cultures de cellules et des permatozoides bovines.
On propose d'appliquer le test d'hemadsorption des erythrocytes comme methode de differenciation des souches des mycoplasmes bovins.
Resumen
Estirpes adsorbentes y no adsorbentes de Mycoplasma bovirhinis
Tres estirpes de Mycoplasma bovirhinis, que se consideran como prototipos, se contrastaron en cuanto a su facultad de adsorber eritrocitos de oveja. En 2 estirpes (CCM M-22 y TH) fueron positivos los resultados obtenidos, mientras que en la cepa N.C.T.C. 10118 resulto negativo.
La estirpe CCM M-22 adsorbia ademas celulas de diversos organos de mamiferos, cultivos celulares y espermatozoides bovinos. La prueba de hemoadsorcion eritrocitica se propone como tecnica de diferenciacion para las cepas bovinas de Mycoplasma.
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