Sputtering deposition of gold nanoparticles onto graphene oxide functionalized with ionic liquids: biosensor materials for cholesterol detection.

Ionic liquid (IL)-functionalized graphene oxide (GO) containing gold nanoparticles (Au NPs) (5.4-5.9 nm) deposited by sputtering combined with cholesterol oxidase appears to be a suitable and efficient biosensor for total cholesterol detection. These biosensors showed good linear range (25-340 μmol L-1) for total cholesterol determination by colorimetric tests. In addition, control experiments in the presence of interferents demonstrated that the hybrid materials provided a selective detection of total cholesterol. The biosensing activities obtained for the hybrid materials indicate that these compounds are potential biosensors for clinical applications.

[1]  N. Tanaka,et al.  Single-step synthesis of gold-silver alloy nanoparticles in ionic liquids by a sputter deposition technique. , 2008, Chemical communications.

[2]  P. Solanki,et al.  A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application. , 2014, Nanoscale.

[3]  Qixian Zhang,et al.  The synthesis of ionic-liquid-functionalized multiwalled carbon nanotubes decorated with highly dispersed Au nanoparticles and their use in oxygen reduction by electrocatalysis , 2008 .

[4]  M. Pumera,et al.  Graphene oxide immobilized enzymes show high thermal and solvent stability. , 2015, Nanoscale.

[5]  Steven Mann,et al.  Differential Effects of Aerobic Exercise, Resistance Training and Combined Exercise Modalities on Cholesterol and the Lipid Profile: Review, Synthesis and Recommendations , 2013, Sports Medicine.

[6]  A Gupta,et al.  Raman scattering from high-frequency phonons in supported n-graphene layer films. , 2006, Nano letters.

[7]  J. Dupont.  From molten salts to ionic liquids: a "nano" journey. , 2011, Accounts of chemical research.

[8]  B. D. Malhotra,et al.  A novel ternary NiFe2O4/CuO/FeO-chitosan nanocomposite as a cholesterol biosensor , 2012 .

[9]  B. D. Malhotra,et al.  Highly Efficient Bienzyme Functionalized Biocompatible Nanostructured Nickel Ferrite–Chitosan Nanocomposite Platform for Biomedical Application , 2013 .

[10]  A. Gopalan,et al.  Development of a stable cholesterol biosensor based on multi-walled carbon nanotubes-gold nanoparticles composite covered with a layer of chitosan-room-temperature ionic liquid network. , 2009, Biosensors & bioelectronics.

[11]  Jay P. Singh,et al.  Bienzyme-functionalized monodispersed biocompatible cuprous oxide/chitosan nanocomposite platform for biomedical application. , 2013, The journal of physical chemistry. B.

[12]  A. Iqbal,et al.  Modifications in development of graphene oxide synthetic routes , 2016 .

[13]  Xu Liu,et al.  Sensitive colorimetric detection of glucose and cholesterol by using Au@Ag core–shell nanoparticles , 2016 .

[14]  Jing Wang,et al.  Enlargement of gold nanoparticles on the surface of a self-assembled monolayer modified electrode: a mode in biosensor design. , 2006, Analytical chemistry.

[15]  D. Weibel,et al.  Sputtering-deposition of Ru nanoparticles onto Al2O3 modified with imidazolium ionic liquids: synthesis, characterisation and catalysis. , 2015, Dalton transactions.

[16]  B. D. Malhotra,et al.  Biocompatible self-assembled monolayer platform based on (3-glycidoxypropyl)trimethoxysilane for total cholesterol estimation , 2011 .

[17]  R. Schlögl,et al.  Is Nanostructuring Sufficient To Get Catalytically Active Au , 2016 .

[18]  Bansi D Malhotra,et al.  Recent advances in cholesterol biosensor. , 2008, Biosensors & bioelectronics.

[19]  Cinzia Casiraghi,et al.  Probing the nature of defects in graphene by Raman spectroscopy. , 2012, Nano letters.

[20]  J. Dupont,et al.  “Imprinting” Catalytically Active Pd Nanoparticles onto Ionic‐Liquid‐Modified Al2O3 Supports , 2013 .

[21]  D. Shan,et al.  Performance-enhanced cholesterol biosensor based on biocomposite system: Layered double hydroxides-chitosan , 2011 .

[22]  R. Ruoff,et al.  The chemistry of graphene oxide. , 2010, Chemical Society reviews.

[23]  M. Gholivand,et al.  Amperometric cholesterol biosensor based on the direct electrochemistry of cholesterol oxidase and catalase on a graphene/ionic liquid-modified glassy carbon electrode. , 2014, Biosensors & bioelectronics.

[24]  M. Crook,et al.  Hypocholesterolaemia in a Hospital Population , 1999, Annals of clinical biochemistry.

[25]  Meihe Zhang,et al.  A biosensor for cholesterol based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence. , 2012, Biosensors & bioelectronics.

[26]  A. Safavi,et al.  Electrodeposition of gold-platinum alloy nanoparticles on ionic liquid-chitosan composite film and its application in fabricating an amperometric cholesterol biosensor. , 2011, Biosensors & bioelectronics.

[27]  Pengfei Cai,et al.  Specific ionic effect for simple and rapid colorimetric sensing assays of amino acids using gold nanoparticles modified with task-specific ionic liquid. , 2016, Analytica chimica acta.

[28]  Sandy Shuo Zhao,et al.  Imidazolium-based ionic liquid surfaces for biosensing. , 2013, Analytical chemistry.

[29]  P. Fu,et al.  Enzymatic determination of total serum cholesterol. , 1974, Clinical chemistry.

[30]  Lixin Xia,et al.  Direct electrochemistry of cholesterol oxidase and biosensing of cholesterol based on PSS/polymeric ionic liquid–graphene nanocomposite , 2016 .

[31]  P. Licence,et al.  Photoelectron spectroscopy of ionic liquid-based interfaces. , 2010, Chemical reviews.

[32]  Xiaoquan Lu,et al.  Two-phase synthesis of hydrophobic ionic liquid-capped gold nanoparticles and their application for sensing cholesterol , 2014 .

[33]  Cellular cholesterol delivery, intracellular processing and utilization for biosynthesis of steroid hormones , 2010, Nutrition & metabolism.

[34]  C. R. Raj,et al.  Development of an Amperometric Cholesterol Biosensor Based on Graphene-Pt Nanoparticle Hybrid Material , 2010 .

[35]  F. Stavale,et al.  Quantifying defects in graphene via Raman spectroscopy at different excitation energies. , 2011, Nano letters.

[36]  M. Pumera,et al.  Functionalization of Hydrogenated Graphene: Transition-Metal-Catalyzed Cross-Coupling Reactions of Allylic C-H Bonds. , 2016, Angewandte Chemie.

[37]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[38]  Huafeng Yang,et al.  Covalent functionalization of polydisperse chemically-converted graphene sheets with amine-terminated ionic liquid. , 2009, Chemical communications.